한국응용곤충학회지 (Korean journal of applied entomology)

  • 제47권3호
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  • Pages.303-308
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  • 2008
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  • 1225-0171(pISSN)
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  • 2287-545X(eISSN)
한국응용곤충학회 (Korean Society of Applied Entomology)
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복숭아순나방(Grapholita molesta) 성페로몬 트랩에 포획된 미동정 나방의 발생패턴과 판별 분자지표

Occurrence Pattern of an Unidentified Moth Captured by Sex Pheromone Trap of the Oriental Fruit Moth, Grapholita molesta, and Its Discriminating Molecular Markers

  • 허혜정 (안동대학교 생명자원과학부 식물의학) ;
  • 손예림 (안동대학교 생명자원과학부 식물의학) ;
  • 김용균 (안동대학교 생명자원과학부 식물의학)
  • Huh, Hye-Jung (School of Bioresource Sciences, Andong National University) ;
  • Son, Ye-Rim (School of Bioresource Sciences, Andong National University) ;
  • Kim, Yong-Gyun (School of Bioresource Sciences, Andong National University)
  • 발행 : 2008.09.30
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초록

복숭아순나방(Grapholita molesta) 성페로몬 트랩에 미동정 나방이 포획되었다. 이들은 주로 봄에 채집되었으며 형태적으로 복숭아순나방과 유사하나 비교적 몸 크기가 커서 육안으로 쉽게 구별할 수 있다. 이들의 발생 패턴도 4-5월의 복숭아순나방 월동세대 발생과 유사하였다. 이 기간 동안 이들은 사과원 내부 보다는 주로 과수원에서 떨어진 곳에 설치된 페로몬 트랩에 집중적으로 포획되었다. 5월 이후 이들 미동정 나방은 복숭아순나방 페로몬 트랩에 더 이상 포획되지 않았다. 이 미동정 나방과 기존의 복숭아순나방과의 계통 분류학적 판별을 위해 분자마커가 개발되었다. 미토콘드리아 cytochrome b 영역을 기반으로 선발된 4종의 PCR-RFLP 분자지표는 이 미동정 나방과 복숭아순나방을 구분할 수 있었다.

An unidentified moth was captured in sex pheromone traps of the oriental fruit moth, Grapholita molesta, especially at spring season in apple orchards and their vicinity. Though the captured males were similar in appearance to G. molesta males, they were easily distinguished by a matted difference in body size. Their occurrence pattern was also similar to that of overwintering G. molesta population from April to May, at which more males were captured in the pheromone traps installed in the vicinity of apple orchards than within apple orchards. After May, they were no longer captured in the pheromone traps. To investigate any larval damage due to this unidentified moth, molecular markers needed to be developed. Four PCR-RFLP markers originated from cytochrome b region of mitochondrial DNA could distinguish this unidentified moth from G. molesta.

키워드

참고문헌

  1. Ahn, S.B., H.W. Koh and Y.I. Lee. 1985. Study on apple pests and natural enemy. Res. Rept. RDA. Crop Protection: 417-428
  2. Bochert, D.M., R.E. Stinner, J.F. Walgenbach and G.G. Kennedy. 2004. Oriental fruit moth (Lepidoptera: Tortricidae) phenology and management with methoxyfenozide in North Carolina apples. J. Econ. Entomol. 97: 1353-1364 https://doi.org/10.1093/jee/97.4.1353
  3. Boo, K.S. 1998. Variation in sex pheromone composition of a few selected lepidopteran species. J. Asia-Pac. Entomol. 1: 17-23 https://doi.org/10.1016/S1226-8615(08)60003-5
  4. Clary, D.O. and D.R. Wolstenholme. 1985. The mitochondrial DNA molecule of Drosophila yakuba: nucleotide sequence, gene organization, and genetic code. J. Mol. Evol. 22: 252-271 https://doi.org/10.1007/BF02099755
  5. Hyun, J.S. 2005. Integrated pest management. Principles and practices. Kor. J. Appl. Entomol. 44: 73-90
  6. Jung, S. and Y. Kim. 2008. Comparative analysis to damage reduction of host plant by applying a mating disruptor of the oriental fruit moth, Grapholita molesta, in two different cultivation environments of apple orchard. Kor. J. Appl. Entomol. 47: 51-57 https://doi.org/10.5656/KSAE.2008.47.1.051
  7. Jung, S., C. Park, M. Park, S. Lee, K. Choi, Y. Hong and Y. Kim. 2006. Efficacy of commercial mating disruptors on field overwintering populations of Oriental fruit moth, Grapholita molesta (Busck). Kor. J. Appl. Entomol. 45: 235-240
  8. Kanga, L.H.B., D.J. Pree, F.W. Plapp, Jr. and J.L. van Lier. 2001. Sex-linked altered acetylcholinesterase resistance to carbamate insecticides in adults of the oriental fruit moth, Grapholita molesta (Lepidoptera; Tortricidae). Pestic. Biochem. Physiol. 71: 29-39 https://doi.org/10.1006/pest.2001.2562
  9. Kim, D.S., K.S. Boo and H.Y. Jeon. 2004. Evaluation of pheromone lure of Grapholita molesta (Lepidoptera: Tortricidae) and forecasting its phenological events in Suwon. Kor. J. Appl. Entomol. 43: 281-289
  10. Kim, Y., M.L. Lee and C. Chung. 1998. Study on the genetic variation of the mitochondrial DNA in the beet armyworm, Spodoptera exigua (Hübner), using PCR-RFLP. Kor. J. Appl. Entomol. 37: 23-30
  11. Ming, Q.L., Y.H. Yan and C.Z. Wang. 2007. Mechanisms of premating isolation between Helicoverpa armigera (Hübner) and Helicoverpa assulta (Guenée) (Lepidoptera: Noctuidae). J. Insect Physiol. 53: 170-178 https://doi.org/10.1016/j.jinsphys.2006.11.007
  12. Pree, D.J., K.J. Whitty, L. van Driel, G.M. Walker and L. Van Driel. 1998. Resistance to insecticides in oriental fruit moth populations (Grapholita molesta) from the Niagara Peninsula of Ontario. Can. Entomol. 130: 245-256 https://doi.org/10.4039/Ent130245-3
  13. Roehrich, R. 1961. Contribution a l'étude écologique des populations de la tordeuse de pêcher (Grapholita molesta Busk) dans la région Aquitaine. Annales des Epiphyties. p.114
  14. Rothschild, G.H.L. and R.A. Vickers. 1991. Biology, ecology and control of the oriental fruit moth. pp. 389-412. In World crop pests, Tortricid pests: their biology, natural enemies and control, Vol. 5. eds. by L.P.S. Van der Geest and H.H. Evenhuis. Elsevier, Amsterdam
  15. Sambrook, J.E., F. Fritsh and T. Maniatis. 1989. Molecular cloning. A laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
  16. Simon, C., F. Frati, A. Beckenbach, B. Crespi, H. Liu and P. Flook. 1994. Evolution, weighting, and phylogenetic utility of mitochondrial gene-sequences and a compilation of conserved polymerase chain-reaction primers. Ann. Entomol. Soc. Am. 87: 651-701 https://doi.org/10.1093/aesa/87.6.651
  17. Usmani, K.A. and P.W. Shearer. 2001. Topical pheromone trap assays for monitoring susceptibility of male oriental fruit moth (Lepidoptera: Tortricidae) populations to azinphosmethyl in New Jersey. J. Econ. Entomol. 94: 233-239 https://doi.org/10.1603/0022-0493-94.1.233

피인용 문헌

  1. Simultaneous identification ofGrapholita molestaBusck andGrapholita dimorphaKomai by PCR-RFLP vol.46, pp.3, 2016, https://doi.org/10.1111/1748-5967.12166
  2. Different types of fruit damages of three internal apple feeders diagnosed with mitochondrial molecular markers vol.16, pp.2, 2013, https://doi.org/10.1016/j.aspen.2013.01.008