Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Lysobacter ginsengisoli sp. nov., a Novel Species Isolated from Soil in Pocheon Province, South Korea

  • Jung, Hae-Min (Department of Biological Sciences, Korea Advanced institute of Science and Technology) ;
  • Ten, Leonid N. (Department of Biology and Medicinal Science, Pai Chai University) ;
  • Im, Wan-Taek (Department of Biological Sciences, Korea Advanced institute of Science and Technology) ;
  • Yoo, Soon-Ae (Department of Biology and Medicinal Science, Pai Chai University) ;
  • Lee, Sung-Taik (Department of Biological Sciences, Korea Advanced institute of Science and Technology)
  • Published : 2008.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

A Gram-negative, aerobic, rod-shaped, nonspore-forming bacterial strain, designated Gsoil $357^T$ was isolated from soil sample of a ginseng field in Pocheon Province (South Korea). The isolate contained Q-8 as the predominant ubiquinone and iso-$C_{16:0}$, iso-$C_{17:1}$ ${\omega}9c$, and iso-$C_{15:0}$ as the major fatty acids. The G+C content of the genomic DNA was 69.3 mol%. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Gsoil $357^T$ was most closely related to Lysobacter gummosus (97.6%) and Lysobacter antibioticus (97.6%). However, the DNA-DNA relatedness value between strain Gsoil $357^T$ and its phylogenetically closest neighbors was less than 17%. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 357T should be classified as representing a novel species in the genus Lysobacter, for which the name Lysobacter ginsengisoli sp. novo is proposed. The type strain is Gsoil $357^T$ (=KCTC $12602^T$=DSM $18420^T$).

Keywords

References

  1. An, D.-S., W.-T. Im, S.-T. Lee, W.-Y. Choi, and M.-H. Yoon. 2007. Chitinophaga soli sp. nov. and Chitinophaga terrae sp. nov., isolated from soil of a ginseng field in Pocheon Province, Korea. J. Microbiol. Biotechnol. 17: 705-711
  2. Bae, H.-S., W.-T. Im, and S.-T. Lee. 2005. Lysobacter concretionis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor. Int. J. Syst. Evol. Microbiol. 55: 1155-1161 https://doi.org/10.1099/ijs.0.63399-0
  3. Buck, J. D. 1982. Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl. Environ. Microbiol. 44: 992-993
  4. Christensen, P. and F. D. Cook. 1978. Lysobacter, a new genus of nonfruiting, gliding bacteria with a high base ratio. Int. J. Syst. Bacteriol. 28: 367-393 https://doi.org/10.1099/00207713-28-3-367
  5. Ezaki T., Y. Hashimoto, and E. Yabuuchi. 1989. Fluorometric DNA-DNA hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int. J. Syst. Bacteriol. 39: 224-229 https://doi.org/10.1099/00207713-39-3-224
  6. Kim, M. K., W.-T. Im, H. Ohta, M. Lee, and S.-T. Lee. 2005. Sphingopyxis granuli sp. nov., a ${\beta}-glucosidase$ producing bacterium in the family Sphingomonadaceae in ${\alpha}-4$ subclass of the Proteobacteria. J. Microbiol. 43: 152-157
  7. Lee, J. W., W.-T. Im, M. K. Kim, and D.-C. Yang. 2006. Lysobacter koreensis sp. nov., isolated from a ginseng field. Int. J. Syst. Evol. Microbiol. 56: 231-235 https://doi.org/10.1099/ijs.0.63955-0
  8. Mesbah, M., U. Premachandran, and W. Whitman. 1989. Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int. J. Syst. Bacteriol. 39: 159-167 https://doi.org/10.1099/00207713-39-2-159
  9. Moore, D. D. 1995. Preparation and analysis of DNA, pp. 2- 11. In F. W. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (eds.), Current Protocols in Molecular Biology. Wiley, New York, U.S.A.
  10. Sasser, M. 1990. Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. MIDI, Newark, DE, U.S.A
  11. Shin, Y. K., J.-S. Lee, C. O. Chun, H.-J. Kim, and Y.-H. Park. 1996. Isoprenoid quinone profiles of the Leclercia adecarboxylata KTCT 1036T. J. Microbiol. Biotechnol. 6: 68-69
  12. Stackebrandt, E. and B. M. Goebel. 1994. Taxonomic note: A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol. 44: 846-849 https://doi.org/10.1099/00207713-44-4-846
  13. Ten, L. N., W.-T. Im, S.-H. Baek, J.-S. Lee, H.-M. Oh, and S.-T. Lee. 2006. Bacillus ginsengihumi sp. nov., a novel species isolated from soil of a ginseng field in Pocheon Province, South Korea. J. Microbiol. Biotechnol. 16: 1554-1560
  14. Ten, L. N., S.-H. Baek, W.-T. Im, Q.-V. Liu, Z. Aslam, and S.-T. Lee. 2006. Bacillus panaciterrae sp. nov., isolated from soil of a ginseng field. Int. J. Syst. Evol. Microbiol. 56: 2861-2866 https://doi.org/10.1099/ijs.0.64403-0
  15. Wayne, L. G., D. J. Brenner, R. R. Colwell, P. A. D. Grimont, O. Kandler, M. I. Krichevshy, et al. 1987. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol. 37: 463-464 https://doi.org/10.1099/00207713-37-4-463
  16. Weon, H.-Y., B.-Y. Kim, Y.-K. Baek, S.-H. Yoo, S.-W. Kwon, E. Stackebrandt, and S.-J. Go. 2006. Two novel species, Lysobacter daejeonensis sp. nov. and Lysobacter yangpyeongensis sp. nov., isolated from Korean greenhouse soils. Int. J. Syst. Evol. Microbiol. 56: 947-951 https://doi.org/10.1099/ijs.0.64095-0
  17. Weon, H.-Y., B.-Y. Kim, M.-K. Kim, S.-H. Yoo, S.-W. Kwon, S.-J. Go, and E. Stackebrandt. 2007. Lysobacter niabensis sp. nov. and Lysobacter niastensis sp. nov., isolated from greenhouse soils in Korea. Int. J. Syst. Evol. Microbiol. 57: 548-551 https://doi.org/10.1099/ijs.0.64473-0
  18. Yassin, A. F., W.-M. Chen, H. Hupfer, C. Siering, R. M. Kroppenstedt, A. B. Arun, et al. 2007. Lysobacter defluvii sp. nov., isolated from municipal solid waste. Int. J. Syst. Evol. Microbiol. 57: 1131-1136 https://doi.org/10.1099/ijs.0.64966-0
  19. Yoon, M.-H, L. N. Ten, and W.-T. Im. 2007. Cohnella panacarvi sp. nov., a xylanolytic bacterium isolated from the ginseng cultivating soil. J. Microbiol. Biotechnol. 17: 913-918