Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Specific Detection of Xanthomonas oryzae pv. oryzicola in Infected Rice Plant by Use of PCR Assay Targeting a Membrane Fusion Protein Gene

  • Kang, Man-Jung (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Shim, Jae-Kyung (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Cho, Min-Seok (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Seol, Young-Joo (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Hahn, Jang-Ho (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Hwang, Duk-Ju (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Park, Dong-Suk (National Institute of Agricultural Biotechnology, Rural Development Administration)
  • Published : 2008.09.30
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Abstract

Successful control of Xanthomonas oryzae pv. oryzicola, the causal agent of bacterial leaf streak, requires a specific and reliable diagnostic tool. A pathovar-specific PCR assay was developed for the rapid and accurate detection ofthe plant pathogenic bacterium Xanthomonas oryzae pv. oryzicola in diseased plant. Based on differences in a membrane fusion protein gene of Xanthomonas oryzae pv. oryzicola and other microorganisms, which was generated from NCBI (http://www.ncbi.nlm.nih.gov/) and CMR (http://cmr.tigr.org/) BLAST searches, one pair of pathovar-specific primers, XOCMF/XOCMR, was synthesized. Primers XOCMF and XOCMR from a membrane fusion protein gene were used to amplity a 488-bp DNA fragment. The PCR product was only produced from 4 isolates of Xanthomonas oryzae pv. oryzicola among 37 isolates of other pathovars and species of Xanthomonas, Pectobacterium, Pseudomonas, Burkholderia, Escherichia coli, and Fusarium oxysporum f.sp. dianthi. The results suggested that the assay detected the pathogen more rapidly and accurately than standard isolation methods.

Keywords

References

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