환경생물 (Korean Journal of Environmental Biology)

  • 제26권1호
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  • Pages.36-41
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  • 2008
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  • 1226-9999(pISSN)
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  • 2287-7851(eISSN)
한국환경생물학회 (Korean Society of Environmental Biology)
권호 표지

DEHP에 노출된 Chironomus riparius의 하순기절에 나타난 기형 유형들

Types of Mentum Deformity for Chironomus riparius Exposure to DEHP

  • 곽인실 (전남대학교 해양기술학부) ;
  • 정경숙 (전남대학교 해양기술학부)
  • Kwak, Inn-Sil (Faculty of Marine Technology, Chonnam National University) ;
  • Jeong, Gyeong-Suk (Faculty of Marine Technology, Chonnam National University)
  • 발행 : 2008.03.30
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초록

실내에서 사육된 C. riparius를 대상으로 내분비계 교란물질인 DEHP를 처리하여 노출시간에 따른 기형발생률과 발생한 기형의 유형을 세분화하였다. 하순기절 구조별 기형발생률은 MLT (medial lateral tooth)에서 가장 높았고 그 다음이 LT (lateral tooth), MIX 순으로 높게 나타났다. 비처리군과 처리군 모두 MLT 단독의 기형이 가장 많았다. 하순기절의 기형유형은 여섯 가지로 세분화하였다. 발생빈도가 높은 기형유형은 tooth 추가(4.3%), tooth 쪼개짐(2.8%), tooth 결실 (2.4%), tooth 합쳐짐 (1.4%), $K"{o}hn$ gap (0.5%)과 형태적 이상(0.5%) 순으로 발생하였다. 약제 처리에서만 관찰된 기형은 tooth 쪼개짐과 tooth 합쳐짐이었다.

Induction of morphological deformities in Chironomus riparius larvae was assessed after exposure to 0.3, 1, 10 and 30 ${\mu}g\;L^{-1}$ potential endocrine disruption chemicals, Diethylhexyl phtalate (DEHP). We observed and subdivide types of mentum deformities and counted the incidence of mentum deformities. The mortality was not increased according to concentrations of DEHP, while was depend on the exposed days. The incidence of mentum deformities for MLT (median lateral teeth), LT (lateral teeth), and MIX (MLT+LT) of the mentum morphological structure showed MLT (2.8$\sim$11.4%)>LT (2.8$\sim$2.9%)>MIX (2.8$\sim$3.0%). The type of mentum deformities on C. riparius was classified six groups following exposure to DEHP. The incidence rate was occupied following to tooth addition (4.3%), tooth split (2.8%), tooth deletion (2.4%), tooth fusion (1.4%), $K"{o}hn$ gap (0.5%) and abnormal shape (0.5%). Especially tooth split and tooth fusion were observed in DEHP treated conditions.

키워드

참고문헌

  1. Brown D, RS Thompson, KM Stewart, CP Croudace and E Gillings. 1996. The effect of phthalate ester plasticisers on the emergence of the midge (Chironomus riparius) from treated sediments. Chemosphere. 32:2177-2187 https://doi.org/10.1016/0045-6535(96)00128-2
  2. Darbas B, J Zdarek, T Timar and MHT El-Din. 1990. Effects of the bipyridylium herbicides diquat dibromide and paraquat dichloride on growth and development of Neobellieria bullata (Diptera: Sarcophagidae) larvae. J. Econ. Entomol. 83:2175-2180 https://doi.org/10.1093/jee/83.6.2175
  3. De Bisthoven LJ, C Huysmans and F Ollevier. 1995. The in situ relationship between sediment concentrations of micropollutants and morphological deformities in Chironomus thummi larvae (Diptera; Chironomidae) from lowland river (Belgium): a spatial comparison. In: Chironomids: from genes to ecosystems (Cranston PS, ed.). Canberra, Australia: Commonwealth Scientific and Industrial Research Organisation (CSIRO)
  4. deFur PL, M Crane, CG Ingersoll and L Tattersfield. 1999. Endocrine Disruption in Invertebrates: Endocrinology, Testing and Assessment. SETAC technical publication, Pensacola, Florida. 303pp
  5. Dickman M, Q Lan and B Matthews. 1990. Teratogens in the Niagara River watershed as reflected by chironomid (Diptera: Chironomidae) labial plate deformities. Water Pollut. Res. J. Can. 24:47-79
  6. Elendt BP and WR Bias. 1990. Trace nutrient deficiency in Daphnia magna cultured in standard medium for toxicity testing; effects of the optimization of culture conditions on life history parameters of Daphnia magna. Water Res. 24:1157-1167 https://doi.org/10.1016/0043-1354(90)90180-E
  7. Gillesby BE and TR Zacharewski. 1998. Exoestrogens: mechanisms of action and strategies for identification and assessment. Environ. Toxicol. Chem. 17:3-14 https://doi.org/10.1897/1551-5028(1998)017<0003:EMOAAS>2.3.CO;2
  8. Hamilton AL and OA Saether. 1971. The occurrence of characteristic deformities in the chrononmid larvae of several Canadian lakes. Can. Entomol. 103:363-368 https://doi.org/10.4039/Ent103363-3
  9. Kosalwat P and KW Knight. 1987. Chronic toxicity of copper to a partial life cycle of the midge, Chironomus decorus. Environ. Contam. Toxicol. 16:283-290 https://doi.org/10.1007/BF01054945
  10. Kwak IS and W Lee. 2003a. Effects of the hormone mimetid insecticide tebufenozide on Chironomus flaviplumus larvae. Korean J. Environ. Biol. 21(1):72-76
  11. Kwak IS and W Lee. 2003b. Effects of the molting-hormone mimetid insecticide tebufenozide on Chironomus riparius larvae. Korean J. Environ. Biol. 21(3):286-291
  12. Kwak IS and W Lee. 2004a. Effects of ecdysteroid agonist tebufenozide on freshwater Chironomids. Korean J. Limnol. 37(1):96-101
  13. Kwak IS and W Lee. 2004b. Detecting points for ecological disruptions and developmental delay exposure to DEHP in Chironomus riparius (Diptera: Chironomidae). Korean J. Environ. Biol. 22(2):321-328
  14. Kwak IS and W Lee. 2005a. Endpoint for DEHP exposure assessment in Chironomus riparius. Environ. Contam. Toxicol. 74:1179-1185 https://doi.org/10.1007/s00128-005-0705-0
  15. Kwak IS and W Lee. 2005b. Two-demensional gel electrophoresis analysis of proteins following tebufenozide treatment of Chironomus riparius. Environ. Contam. Toxicol. 74:1159-1165 https://doi.org/10.1007/s00128-005-0702-3
  16. Kwak IS and W Lee. 2005c. Mouthpart deformity and developmental retardation exposure of Chironomus plumosus (Diptera: Chironomidae) to tebufenozide. Environ. Contam. Toxicol. 75:859-865 https://doi.org/10.1007/s00128-005-0829-2
  17. Kwak IS and W Lee. 2007. The mentum deformity of C. riparius following exposure to bisphenol A and 4-nonylphenol. Korean J. Environ. Biol. 25(1):66-71
  18. Milbrink G. 1983. Characteristic deformities in tubificid oligochaetes inhabiting polluted bays of Lake Vanern, Southern Sweden. Hydrobiologia. 106:169-184 https://doi.org/10.1007/BF00006749
  19. Patlak M. 1996. A testing deadline for endocrine disruptors: EPA scrambles to develop a screening program for these complex substances. Environ. Sci. Technol. 30:540A-544A https://doi.org/10.1021/es962527a
  20. Pinder LCV, TG Pottinger, Z Billinghurst and MH Depledge. 1999. Endocrine function in aquatic invertebrates and evidence for disruption by environment pollutants, R & D report TR E67. Bristol, UK: Environment Agency and Endocrine Modulators Steering Group, 150
  21. Santillo D, RL Stringer, PA Johnston and J Tickner. 1998. The precautionary principle: protecting against failures of scientific method and risk assessment. Mar. Pollut. Bull. 36:939-950 https://doi.org/10.1016/S0025-326X(98)80003-9
  22. Sheehan DS and WS Branham. 1987. Dissociation of estrogen induced uterine growth and ornithine decarboxylase activity in the post natal rat. Teratogen. Carcin. Mut. 7:411-422 https://doi.org/10.1002/tcm.1770070408
  23. US EPA. 2004. "National Primary Drinking Water Regulations: Consumer Factsheet on: Phthalate, di(2-ethylhexyl)," Office of Water, Office of Ground Water and Drinking Water. EPA 811-F-95-003-T
  24. Vermeulen AC, G Liberloo, P Dumont, F Ollevier and BR Goddeeris. 2000. Exposure of Chironomus riparius larvae (Diptera) to lead, mercury and $\beta$-sitosterol: effects on mouthpart deformation and moulting. Chemosphere. 41:1581-1591 https://doi.org/10.1016/S0045-6535(00)00033-3
  25. Warwick WF. 1989. Morphological deformities in larvae of Procladius skuse (Diptera: Chironomidae) and their biomonitoring potential. Can. J. Fish Aquat. Sci. 46:1255-1271 https://doi.org/10.1139/f89-162
  26. Warwick WF 1991. Indexing deformities in ligulae and antennae of Procladus larvae (Diptera: Cimonomidae); Application to contaminant-stressed environments. Can. J. Aquat. Sci. 48:1151-1156 https://doi.org/10.1139/f91-139
  27. Watts MM, D Pascoe and K Carroll. 2001. Chronic exposure to 17$\alpha$-ethinylestradiol and bisphenol a effects on development and reproduction in the freshwater invertebrate Chironomus riparius (Diptera: Chironomidae). Aquat. Toxicol. 55:113-124 https://doi.org/10.1016/S0166-445X(01)00148-5
  28. Watts MM, D Pascoe and K Carroll. 2003. Exposure to 17$\alpha$- ethinylestradiol and bisphenol A-effects on larval moulting and mouthpart structure of Chironomus riparius. Ecotoxicol. Environ. Saf. 54:207-215 https://doi.org/10.1016/S0147-6513(02)00029-5
  29. West CW, GT Ankley, JW Nichols, GE Elonen and DE Nessa. 1997. Toxicity and bioaccumulation of 2,3,7,8-tetrachlorodibenzo-p-dioxin in long-term tests with the freshwater benthic invertebrates Chironomus tentans and Lumbriculus variegatus. Environ. Toxicol. Chem. 16:1287-1294 https://doi.org/10.1897/1551-5028(1997)016<1287:TABOTP>2.3.CO;2