Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Proteomic Analysis of Fructophilic Properties of Osmotolerant Candida magnoliae

  • Yu, Ji-Hee (Department of Agricultural Biotechnology and the BIO-MAX Institute, Seoul National University) ;
  • Lee, Dae-Hee (Department of Agricultural Biotechnology and the BIO-MAX Institute, Seoul National University) ;
  • Park, Yong-Cheol (Center for Agricultural Biomaterials, Seoul National University) ;
  • Lee, Mi-Gi (Department of Agricultural Biotechnology and the BIO-MAX Institute, Seoul National University) ;
  • Kim, Dae-Ok (Department of Food Science and Technology, Kyung Hee University) ;
  • Ryu, Yeon-Woo (Department of Molecular Science and Technology, Ajou University) ;
  • Seo, Jin-Ho (Department of Agricultural Biotechnology and the BIO-MAX Institute, Seoul National University)
  • Published : 2008.02.29
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Abstract

Candida magnoliae, an osmotolerant and erythritol producing yeast, prefers D-fructose to D-glucose as carbon sources. For the investigation of the fructophilic characteristics with respect to sugar transportation, a sequential extraction method using various detergents and ultracentrifugation was developed to isolate cellular membrane proteins in C. magnoliae. Immunoblot analysis with the Pma1 antibody and two-dimensional electrophoresis analysis coupled with MS showed that the fraction II was enriched with membrane proteins. Eighteen proteins out of 36 spots were identified as membrane or membrane-associated proteins involved in sugar uptake, stress response, carbon metabolism, and so on. Among them, three proteins were significantly upregulated under the fructose supplying conditions. The hexose transporter was highly homologous to Ght6p in Schizosaccharomyces pombe, which was known as a predominant transporter for the fructose uptake of S. pombe because it exhibited higher affinity to D-fructose than D-glucose. The physicochemical properties of the ATP-binding cassette transporter and inorganic transporter explained their direct or indirect associations with the fructophilic behavior of C. magnoliae. The identification and characterization of membrane proteins involved in sugar uptake might contribute to the elucidation of the selective utilization of fructose to glucose by C. magnoliae at a molecular level.

Keywords

References

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