Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Inactivation of Listeria monocytogenes in Brine and Saline by Alternating High-Voltage Pulsed Current

  • Lee, Mi-Hee (Department of Medical Engineering, Yonsei University College of Medicine) ;
  • Han, Dong-Wook (Department of Medical Engineering, Yonsei University College of Medicine) ;
  • Woo, Yeon-I. (Department of Medical Engineering, Yonsei University College of Medicine) ;
  • Uzawa, Masakazu (Applied Science Co. Ltd.) ;
  • Park, Jong-Chul (Department of Medical Engineering, Yonsei University College of Medicine)
  • Published : 2008.07.31
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Abstract

The inactivating efficiency of alternating high-voltage pulsed (AHVP) current was investigated in brine (20 w/v% NaCl) and saline (0.9 w/v% NaCl) inoculated with $1\times10^7$ cells/ml of Listeria monocytogenes. AHVP current at 12 V with 1 pulse completely inactivated L. monocytogenes in brine within 3 ms, while the bacteria in saline were fully inactivated by 10-pulsed electric treatment at 12 V within the same time. Electron microscopic observation demonstrated substantial structural damage of electrically treated L. monocytogenes in brine. These results suggest that AHVP treatment would be effective for the rapid and complete inactivation of L. monocytogenes in brine or saline solution.

Keywords

References

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