Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of Bulnesia sarmienti Water Extracts on Lipid Metabolism in Neonatally Streptozotocin- Induced Diabetic Rats.

신생흰쥐의 Streptozotocin 당뇨 유발에서 Bulnesia sarmienti 열수추출물이 지질대사에 미치는 영향

  • Park, Chang-Ho (Daegu Technopark Bio Industry Center) ;
  • Lee, Gee-Dong (Daegu Gyeongbuk Institute for Oriental Medicine Industry) ;
  • Kim, Jung-Ok (Daegu Gyeongbuk Institute for Oriental Medicine Industry) ;
  • Kim, Kil-Soo (College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Won-Young (Department of Food Engineering, Kyungpook National University) ;
  • Hong, Joo-Heon (Daegu Technopark Bio Industry Center)
  • 박창호 ((재)대구테크노파크 바이오산업지원센터) ;
  • 이기동 (대구경북한방산업진흥원) ;
  • 김정옥 (대구경북한방산업진흥원) ;
  • 김길수 (경북대학교 수의과대학) ;
  • 이원영 (경북대학교 식품공학과) ;
  • 홍주헌 ((재)대구테크노파크 바이오산업지원센터)
  • Published : 2008.07.30
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Abstract

The effects of Bulnesia sarmienti water extract on lipid metabolism were investigated in type-2 diabetes rats. The rats were fed a basal diet and B. sarmienti water extract for 8 weeks. The rats were randomly assigned to each treatment group : normal, control, two kinds of treatment group (BW1: 0.9 mg/ml, BW2: 1.8 mg/ml). After 8 weeks of experimental diets consumption, the body weights and food efficiency ratio of B. sarmienti water extract fed groups were not different from those in the control group. The concentrations of total-cholesterol, LDL-cholesterol, and triglyceride in blood plasma were significantly decreased in the B. sarmienti water extract groups comparison to the control group. Although blood glucose control effects were not observed, the B. sarmienti water extract feeds were highly effective in lowering the levels of blood lipid substances such as triglycerides, total-cholesterol, and LDL-cholesterol.

인슐린 저항성에 기인하여 발병되는 제 2형 당뇨를 유발시킨 흰쥐로부터 8주간 B. sarmienti 열수추출물 급여 후 혈당 및 혈중지질 변화를 알아보았다. 시험기간 동안 음수량, 사료 섭취량, 혈당 모두 정상군과 비교하여 당뇨실험군에서 8주동안 유의적으로 높게 지속되었다. 따라서 제 2형 당뇨모델에서 B. sarmienti 열수추출물의 혈당개선효과는 나타나지 않는 것으로 조사되었다. 그러나 혈중 총콜레스테롤, HDL-콜레스테롤, LDL-콜레스테롤 함량 변화를 측정한 결과 정상군과 비교할 때 당뇨대조군에서는 상대적으로 증가하였으나, B. sarmienti 열수추출물 급여로 유의적인 감소현상과 함께 정상군과 비슷한 수준을 유지 하였다. 특히 B. sarmienti 열수추출물 급여에 따른 혈중 총콜레스테롤 변화는 BW1 34.3%, BW2 43.8%로 평균 39.0%의 감소효과를 나타내었다. 또한 혈중 중성지방 변화는 BW1 48.5%, BW2 47.2%로 평균 47.9%의 감소현상을 나타내었다. 따라서 B. sarmienti 열수추출물의 농도 의존적인 변화는 관찰할 수 없었으나, 혈중 지질 개선효과가 있는 것으로 조사되었다. 본 연구에서 제 2형 당뇨모델에서 8주 동안 B. sarmienti 열수 추출물을 투여하면서 당뇨병의 주요증세인 다갈(polydipsia), 다뇨(polyuria), 다식(polyphagia), 고형당의 혈당조절 효과는 관찰할 수 없었으나, 고혈당을 중심으로 지질대사 이상과 함께 합병증 유발이 문제시 되고 있는 당뇨환자의 체내 지질대사 개선에는 효과를 기대할 수 있을 것으로 사료된다.

Keywords

References

  1. Abate, N. 2000. Obesity and cardiovascular disease. Pathogenetic role of the metabolic syndrome and therapeutic implications. J. Diabetes Complications 14, 154-174. https://doi.org/10.1016/S1056-8727(00)00067-2
  2. Castelli, W. P., P. F. Wilson, D. Lery and K. Anderson. 1990. Serum lipids and risk of coronary artery disease. Atherosclerosis Rev. 21, 7-19.
  3. Cho, Y. J. and M. A. Bang. 2004. Hypoglycemic and antioxidative effects of dietary sea-tangle extracts supplementation in streptozotocin-induced diabetic rats. Kor. J. Nur. 37, 5-14.
  4. De, Leo. 1997. Iron modulation of LPS-induced manganese superoxide dismutase gene expression in rat tissues. FEBS Lett. 406, 131-135. https://doi.org/10.1016/S0014-5793(97)00255-X
  5. Haffner, S. M., S. Lehto, T. Ronnemaa, K. Pyorala, M. Laakso. 1998. Mortality from coronary heart disease in subjects with type 2 diabets and in nondiabetic subjects with and without prior myocardial infarction. N. Engl. J. Med. 339, 229-234. https://doi.org/10.1056/NEJM199807233390404
  6. Jo, D. H., K. J. Mim and C. G. Cha. 2007. The antioxidant and antitumor effects of the extract of Bulnesia sarmienti. J. Fd Hyg. Safety 22, 120-126.
  7. Kim, G. J., Y. G. Kim and H. S. Kim. 1999. Effect of Perilla frutescens extract on the detoxification enzyme activity of hepatic lipid peroxidation in streptozotocin-induced rats. J. Agri. Tech. & Dev. Inst. 3, 1-5.
  8. Kim, H. K., D. W. Cho and Y. T. Hahm. 2000. The effects of Coix bran on lipid metabolism and glucose challenge in hyperlipidemic and diabetic rats. J. Korean Soc. Food Sci. Nutr. 9, 140-146.
  9. Kim, H. S. and S. Y. Chung. 1992. Effects of feeding mixed oils of butter, sardine and safflower on the lipid components in serum and activities of hepatic functional enzyme in rats. J. Kor. Soc. Food Nutr. 21, 608-616.
  10. Kim, H. S., S. H. Kim, G. H. Kim, W. J. Choi and S. Y. Chung. 1993. Effects of the feeding mixed oils with various level of n-3 and n-6 polyunsaturated fatty acid on the lipid components of liver, brain, testis and kidney in dietary hyperlipidemic rats. J. Kor. Soc. Food Nutr. 22, 685-691.
  11. Ko, Y. C. 2003. Effects of muti-extracts of mori folium and regular exercise on lipid metabolism in streptozotocin-induced diabetic rats. Korea Sport Research. 14, 2251-2168.
  12. Koh, J. B. and M. A. Choi. 1999. Effect of tea Fungus/ Kombucha beverage on lipid metabolism in streptozotocin- induced diabetic male rats. J. Korean Soc. Food Sci. Nutr. 28, 613-618.
  13. Koivisto, V. A. 1993. Insulin therapy in type II diabetes. Diabetes Care 16, 29-39. https://doi.org/10.2337/diacare.16.3.29
  14. Laybutt, D. R., H. Kaneto, W. Hasenkamp, S. Grey, J. C. Jonas, A. Groff, C. Ferran, W. S. Bonner, A. Shama and G. C. Weir. 2002. Increased expression of antioxidant and antiapoptotic genes in islets that may contribute to $\beta$-cell survival during chronic hyperglycemia. Diabetes 51, 413-423. https://doi.org/10.2337/diabetes.51.2.413
  15. Lee, T. H. 1999. Diagnosis and classification of diabetes mellitus. Food Indus. and Nutr. 4, 61-65.
  16. Like, A. A., M. C. Appe and A. A. Rossin. 1982. Autoantibodies in the BB/W rat. Diabetes 31, 816-820. https://doi.org/10.2337/diabetes.31.9.816
  17. Lim, S. J. and M. H. Lee. 2005. Effect if fractions of Benincasa hispida on plasma levels of glucose and lipid in streptozotocin induced diabetic rats. Korean Nutr. Soc. 38, 801-806.
  18. Macrae, R., R. K. Robinson and M. J. Sadler. 1993. Encyclopedia of food science, food technology and nutrition, Vol. II, pp. 1329. Academic Press, New York.
  19. Mayer-Davis, E. J., R. J. D'Agostino, M. Sad and R. N. Bergaman. 1998. Effect of exercise training on glucose homestasis in normal and insulin deficient diabetic rats. J. Appl. Physiol. 65, 844-851.
  20. Morris, R. D., D. L. Rimm, A. J. Hartz, R. K. Kalkhoff and A. A. Rimm. 1989. Obesity and heredity in the etiology of non-insulin dependent diabetes mellitus in 33, 662 adult white women. Am. J. Epidemiol, 130, 112-121. https://doi.org/10.1093/oxfordjournals.aje.a115302
  21. Nam, H. O., Y. H. Kim, K. W. Lee and C. H. Son. 2003. Effects of the administration of GnRH and HCG on the fetus in pregnant rats. J. Vet. Clin. 20, 212-219.
  22. Pain, V. M. and P. Garlick. 1974. Effect of streptozotocin diabetes and insulin treatment on the rate of protein synthesis in tissues of the rat in vivo. J. Biol. Chem. 249, 4510-4514.
  23. Park, C. H., J. H. Hong, J. O. Kim and G. D. Lee. 2008. Single oral-dose toxicity of Bulnesia sarmienti single and Mixed Extracts in Sprague-Dawley rats. Kor. J. Lab. Anim. Res. 24, 83-86.
  24. Park, S. H., Y. K. Lee and H. S. Lee. 1994. The effects of dietary fiber feeding on gastrointestinal functional and lipid glucose metabolism in streptozotocin induced diabetic rats. Kor. J. Nutr. 27, 311-322.
  25. Poulter N. 1999. Coronary heart disease is a multifactorial disease. Am. J. Hypertens. 12, S92-S95.
  26. The expert committee on the diagnosis and classification of diabetes mellitus. 1997. Report of the expert committee on the diagnosis and classification of diabetes mellitus. Diabets Care. 20 (suppl. 7), 1183-1197. https://doi.org/10.2337/diacare.20.7.1183
  27. Tol, A. V. 1977. Hypertriglyceride in the diabetic rats effective removal of serum very low density lipoprotein. Atherosclerosis 26, 117-128. https://doi.org/10.1016/0021-9150(77)90146-0