Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Optimal Culture Conditions on the Keratinase Production by Bacillus sp. SH-517.

Bacillus sp. SH-517에 의한 keratinase의 생성 최적 배양 조건

  • Bang, Byung-Ho (Division of Food Science, Eulji University) ;
  • Rhee, Moon-Soo (Korea Research Institute of Bioscience and Biotechnology) ;
  • Lim, Ki-Hwan (Department of Microbial Engineering, Konkuk University) ;
  • Yi, Dong-Heui (Department of Microbial Engineering, Konkuk University)
  • 방병호 (을지대학교 식품과학부) ;
  • 이문수 (한국생명공학연구원) ;
  • 임기환 (건국대학교 미생물공학과) ;
  • 이동희 (건국대학교 미생물공학과)
  • Published : 2008.06.30
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Abstract

A strain SH-517 which produce extracellular keratinase, was isolated from the soil of a poultry waste and a poultry factory. An isolate SH-517 was identified as Bacillus sp. based on its morphological and biochemical characteristics. The optimal culture conditions for the production of keratinase by Bacillus sp. SH-517 were investigated. The optimal medium composition for keratinase production was determined to be 2.0% chicken feather as carbon source, 0.5% beef extract as organic nitrogen source, 0.5% $KNO_3$ as inorganic nitrogen source and 0.06% KCl, 0.05% NaCl, 0.04% $KH_2PO_4$, 0.03% $K_2HPO_4$ as mineral source and 0.01% yeast extract as growth factor. The optimal temperature and pH of medium were shown $40^{\circ}C$ and 8.5 with shaking culture (180 rpm/min), respectively. The maximum keratinase production reached maximum of 125 units/ml/min after 42 hr of cultivation under the optimal culturing conditions.

경기도와 충청도 일대의 가금류 처리공장과 폐기물 처리장 부근 토양으로부터 protease 활성이 높은 균주를 분리한 후 그 중 keratinase 활성이 높은 1균주를 최종 선별하여 동정하고 효소생산을 위한 최적 배양조건을 검토한 결과는 다음과 같다. 선별된 균주는 형태학적, 생화학적 특성을 조사한 결과 Bacillus 속으로 판명되었으며, 편의상 Bacillus sp. SH-517로 명명하여 사용하였다. Bacillus sp. SH-517에 의한 keratinase 생성 최적 조건을 검토하였는데, 최적 배지 조성은 탄소원으로 chicken feather 2.0%, 유기질소원으로 beef extract 0.5%, 무기질원소원으로 $KNO_3$ 0.5%, 무기염으로 KCl 0.06%, NaCl 0.05%, $KH_2PO_4$ 0.04%. $K_2HPO_4$ 0.03%이었고 그리고 생육인자로 yeast extract 0.01%이였다. 진탕배양 시(180 rpm/min), 최적 온도와 배지의 pH는 각각 $40^{\circ}C$, 8.5로 나타났으며, 위와 같은 최적 조건 하에서 keratinase의 최대 활성은 42시간 만에 125 units/ml/min이었다.

Keywords

References

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