International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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The effect of UNCL inactivation on the expression of mechanical stress related genes in cultured human PDL fibroblasts

  • Choi, Yong-Seok (Oral Biology Research Institute and The Second stage of BK21, College of Dentistry, Chosun University) ;
  • Jang, Hyun-Sun (Oral Biology Research Institute and The Second stage of BK21, College of Dentistry, Chosun University) ;
  • Lee, Dong-Seol (Dental Research Institute and Department of Oral Histology-Developmental Biology, School of Dentistry, Seoul National University) ;
  • Kim, Heung-Joong (Oral Biology Research Institute and The Second stage of BK21, College of Dentistry, Chosun University) ;
  • Park, Jong-Tae (Oral Biology Research Institute and The Second stage of BK21, College of Dentistry, Chosun University) ;
  • Bae, Hyun-Sook (Department of Dental Hygiene, Namseoul University) ;
  • Park, Joo-Cheol (Dental Research Institute and Department of Oral Histology-Developmental Biology, School of Dentistry, Seoul National University)
  • Published : 2008.06.30
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Abstract

A mutation of UNCL, an inner nuclear membrane RNAbinding protein, has been found to eliminate mechanotransduction in Drosophila. UNCL is expressed in human periodontal tissue including in periodontal ligament (PDL) fibroblasts. However, it is unclear how a mechanical stimulus is translated into cellular responses in PDL fibroblasts. The aim of this study was to evaluate the effect of UNCl on mechanical stress related genes in PDL fibroblasts in response to mechanical stress. The mRNA of TGF-$\beta$, COX-2, and MMP-2 was up-regulated after UNCL inactivation in PDL fibroblasts under the compression force. Under the tensile force, inactivation of UNCL decreased the expression of Biglycan, RANKL, MMP-2, and TIMP-2 mRNAs while it increased the expression of TIMP-1. p38-MAPK was expressed in PDL fibroblasts under compression forces whereas phospho-ERK1/2, p65-NFkB, and c-fos were expressed under tension forces. The expression and phosphorylation of the mechanical stress related genes, kinases, and transcription factors were changed according to the types of stress. Furthermore, most of them were regulated by the inactivation of UNCL. This suggests that UNCL is involved in the regulation of mechanical stress related genes through the signaling pathway in PDL fibroblasts.

Keywords

References

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