The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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The Effect of the Salvia miltiorrhiza on Axon Regeneration Following Central Nervous System Injury

단삼(丹蔘)이 손상된 뇌신경세포에 미치는 영향

  • Shim, Ha-Na (Department of Oriental Internal Medicine, Wonkwang University, College of Oriental Medicine) ;
  • Seong, Kee-Moon (Department of Oriental Internal Medicine, Wonkwang University, College of Oriental Medicine) ;
  • Moon, Seong-Jin (Department of Oriental Internal Medicine, Wonkwang University, College of Oriental Medicine) ;
  • Lee, Seung-Hee (Department of Oriental Internal Medicine, Wonkwang University, College of Oriental Medicine) ;
  • Yang, Jae-Hoon (Department of Oriental Internal Medicine, Wonkwang University, College of Oriental Medicine) ;
  • Song, Bong-Keun (Department of Oriental Internal Medicine, Wonkwang University, College of Oriental Medicine)
  • 심하나 (원광대학교 한의과대학 내과학교실) ;
  • 성기문 (원광대학교 한의과대학 내과학교실) ;
  • 문성진 (원광대학교 한의과대학 내과학교실) ;
  • 이승희 (원광대학교 한의과대학 내과학교실) ;
  • 양재훈 (원광대학교 한의과대학 내과학교실) ;
  • 송봉근 (원광대학교 한의과대학 내과학교실)
  • Published : 2008.06.30
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Abstract

Object: Reactive gliosis that is induced by central nervous system (CNS) injury is involved with up-regulation of CD81 and GFAP. The present study was to examine the effect of the Salvia miltiorrhiza on CD81 and GFAP regulation following brain injury. Methods: Immunoblot and ELISA methods were used to define the level of CD81 and GFAP in the astrocyte cultured from rat brain. Then immunohistochemistry was used to detect CD81 and GFAP in the injured rat brain. Results: The following results were obtained. 1. We did western blot and ELISA to detect the protein isolated from the whole cell and they showed that CD81 and GFAP decreased. 2. We injected Salvia miltiorrhiza extract intravenously to brain-injured rats for 7 days and 30 days, and the immunohistochemistry analyses showed that CD81 and GFAP decreased significantly. Conclusion: These results indicate that Salvia miltiorrhiza could suppress the reactive gliosis, which disturbs the neural regeneration following CNS injury, by controlling the expression of CD81 and GFAP.

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