The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Effects of Job's Tears(Yul-Moo) Extracts on Mouse Splenocyte and Macrophage Cell Activation

율무 추출물의 마우스 비장세포와 대식세포 활성 효과

  • Ryu, Hye-Sook (Dept. of Food and Nutrition, College of Health Sciences, Sangji University)
  • 류혜숙 (상지대학교 보건과학대학 식품영양학과)
  • Published : 2008.03.31
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Abstract

Job's Tears(Yul-Moo) is a grass crop long-used as a traditional medicine; it is also a nourishing food. There are reports of its anti-inflammatory, stomachic, antiallergic activity, and antispastic effects and Job's Tears has been used in China to treat rheumatism, and neuralgia although its warts, rheumanism remains unclear. Thus, the present study was performed to investigate the in vitro effect of Job's Tears extracts on immune function. Here mouse splenocyte proliferation and cytokine production$(IL-1{\beta},\;IL-6,\;TNF-{\alpha})$ by peritoneal macrophages cultured with ethanol and water extracts of Job's Tears were examined. splenocytes proliferation increased with Job's Tears water extracts supplement at concentrations investigated The cytokine production$(IL-1{\beta},\;IL-6,\;TNF-{\alpha})$ by ELISA using a cytokine kit And $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ production increased water extracts supplementation. This in vitro study suggests that supplementation with Job's Tears water extracts may enhance immune function by regulating the splenocyte proliferation and enhancing cytokine production of activated macrophages.

Keywords

References

  1. Ji, WD, Jeong, HC, Lee, SJ and Chun, YG. Antimicrobial activity and distilled components of garlic and ginger. J. Agric. Chem. Biotechnol. 40:514-518. 1997
  2. Pyo, MY and Su, MH. Effects of Phellinus linteus extracts on the humoral immune response in normal and cyclophosphamide-treated mice. J. Applied Phamcol. 9:194-200. 2001
  3. Park, JS and Chyun, JH. Effects of low fat diet and saturated fat supplementation on the immune status of BALB/c mouse. Kor. J. Nutr. 26:578-585. 1993
  4. Wagner, H. Search for plant derived natural products with immunostimulatory activity. Pure & Appl. Chem. 7:1271-2178. 1990
  5. Kim, GH and Sunwoo, YK. Effects of small water dropwort extract on cellular immune response of mice. J. Bacteriolo. Virol. 28:419-430. 1993
  6. Park, HA. Enhancing effect of Ixeris sonchifolia Hance Oenanthe javanica, and Fagopyrum escluentum Moench on mouse immune cell activation. MS. Thesis, Sookmyung Women's Uni., Seoul. Korea. 2003
  7. Ryu, HS and Kim, HS. Effects of job's tear extracts on mouse immune cell ativation. J. Kor. Diet Assoc. 11:44-50. 2005
  8. Park, KY and Rhee, SH. The antitumor effect in Sarcoma-180 tumor cell of mice of mice administered with Japanese apricot, garlic ginger doenjang. J. Kor. Food. Cookery Sci. 21:599-606. 2005
  9. Yun, HJ. Effect of Hizikia fusiforme water extracts on mouse immune cell activation. MS. Thesis, Sookmyung Women's Uni., Seoul. Korea. 2003
  10. Ko, MS, Shin, KM and Lee, MY. Effects of Hizikia fusiforme ethanol extract on antioxidative enzymes in ethanol induced hepatotoxicity of rat liver. J. Kor. Soc. Food. Sci. Nutr. 31:87-91. 2002 https://doi.org/10.3746/jkfn.2002.31.1.087
  11. Yoo, TJ. Sikpoombogam, pp.288-290. Moonundang. Seoul. Korea. 1998
  12. Tanimura, A. Studies on the antitumor component in the seeds of Coix lachryma jobi l. var. mayuen Stapf. II. The structure of coixenolide, Chem. Phar. Bull. 9:47-53. 1961 https://doi.org/10.1248/cpb.9.47
  13. Numata, MA, Yamamoto, M and Yamada, H. Antitumor components isolated from the Chinese herbal medicine Coix lachryma-jabi L. Planta Med. 60:356-359. 1994 https://doi.org/10.1055/s-2006-959500
  14. Kond, YK, Nonno, C and Hikino, H. Isolation of ovulatory-active substances from crops of Job's tears(Coix lachryma jobi L. var. ma-yuen Stapf). Che. Pharm. Bull. 36:3147-3152. 1998
  15. Takahashi, M and Konno, CH. Isolation and hypoglycemic activity of coixans A, B and C glycans of Coix lachryma-jobi L. var. mayuen Stapf seeds. Planta Med. Fed. 1:64-65. 1986
  16. Park, Y, Suzuki, H and Lee, YS. Effect of coxi on plasma, liver and fecal lipid components in the rat fed on lard or soybean oil-cholesterol diet. Biochem. Med. Metab. Biol. 39:7-1. 1988
  17. Ary, MB, Richardson, M and Shewry, PR. Purification and characterization of an insect alpha-amylase inhibitor/endochitinase from seeds of Job's tears. Biochem. Biophys. Acta. 999:260-266. 1988
  18. Mishell, BB and Shiigi, SM. Selected methods in cellular immunology, 1st ed, pp.4-27. WHFreeman and Co, Sanfrancisco. USA. 1980
  19. Kim, HP, Son, KH, and Kang, SS. Anti-inflammatory plant flavonoids and cellular action mechanisms. J. Pharmacological Sci. 96:229-245. 2004 https://doi.org/10.1254/jphs.CRJ04003X
  20. Barnes, PJ and Liew, FY. Nitric oxide and asthmatic inflammation. Immunology Today. 16:128-130. 1995 https://doi.org/10.1016/0167-5699(95)80128-6
  21. Kim, J. Enhancing effect of Paeonia japonica, Houttuynia cordata, and Aster scaber extracts on the immunoreactivity in vivo in mice. Ph.D. Thesis, Sookmyung Women's Uni., Seoul. Korea. 2003
  22. Ryu, HS, Kim, J and Kim, HS. Enhancing effect of Sorghum bicolor L. Moench(Sorghum, su-su) extracts on mouse spleen and macrophage cell activation. Kor. J. Food & Nutr. 19:176-182. 2006
  23. Balkwill, FR, Maylor, MS and Malik S. Tumor necrosis factor as an anticancer agent. Eur. J. Cancer. 26:641-644. 1990 https://doi.org/10.1016/0277-5379(90)90097-D
  24. Chao, CC, Hu, S, Molitor, TW, Brosnan, CF and Berman, JW. Cytokine prodution by human fetal microglia and astrocytes. J. Immunol. 150:2659-2660. 1998
  25. Lee, YS, Ryu, HS and Kim, HS. Effects of elm bark(Ulmus davidiana var. japonica) extracts on modulation of immunocompetence in mice. J. Medicinal Food. 10:118-125. 2006