Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Chiisanoside, A Lupane Triterpenoid from Acanthopanax Leaves, Stimulates Proliferation and Differentiation of Osteoblastic MC3T3-E1 Cells

  • Choi, Eun-Mi (College of Pharmacy, Chungnam National University) ;
  • Ding, Yan (College of Pharmacy, Chungnam National University) ;
  • Nguyen, Huu Tung (College of Pharmacy, Chungnam National University) ;
  • Park, Sang-Hyuk (College of Pharmacy, Chungnam National University) ;
  • Nguyen, Xuan Nhiem (College of Pharmacy, Chungnam National University) ;
  • Liang, Chun (College of Pharmacy, Chungnam National University) ;
  • Lee, Jung-Joon (Molecular Cancer Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Young-Ho (College of Pharmacy, Chungnam National University)
  • Published : 2008.03.31
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Abstract

The leaves of Acanthopanax species have traditionally been used as a tonic and a sedative as well as in the treatment of rheumatism and diabetes. Chiisanoside is the major active lupane triterpenoid of Acanthopanax leaves. To investigate the bioactivities of chiisanoside, which act on bone metabolism, the effects of chiisanoside on the function of osteoblastic MC3T3-E1 cells were studied. Chiisanoside $(0.02{\sim}20\;{\mu}M)$ significantly increased the growth of MC3T3-E1 cells and caused a significant elevation of alkaline phosphatase (ALP) activity, collagen content, and nodules mineralization in the cells (P < 0.05). The effect of chiisanoside (2 ${\mu}M$) in increasing ALP activity was completely prevented by the presence of tamoxifen, suggesting that the effect of chiisanoside might be partly estrogen receptor mediated. Moreover, cotreatment of p38 inhibitor SB203580 or JNK inhibitor SP600125 inhibited chiisanoside-mediated ALP upregulation, suggesting that the induction of differentiation by chiisanoside is associated with increased activation of p38 and JNK mitogen-activated protein kinases. Our data indicate that the enhancement of osteoblast function by chiisanoside may result in the prevention for osteoporosis.

Keywords

References

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