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Korean Mistletoe (Viscum album Coloratum) Extract Induces Eel (Anguilla japonica) Non-specific Immunity

  • Yoon, Taek-Joon (Department of Food and Nutrition, Yuhan College) ;
  • Park, Kwan-Ha (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Choi, Sang-Hoon (Department of Aquatic Life Medicine, Kunsan National University)
  • Published : 2008.12.30
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Abstract

Background: The immunomodulatory effects of Korean mistletoe (Viscum album Coloratum) on the innate immune responses of eel (Anguilla japonica) were studied. Methods: Mistletoe, Freund’s complete adjuvant (FCA), or phosphate-buffered saline (PBS) as a control was injected into eel peritoneal cavities. Results: Nitroblue tetrazolium (NBT)-positive cells in the head kidney of eel were significantly augmented by the second day post-injection of mistletoe. Reactive oxygen intermediates (ROI) were more produced in mistletoe-injected fish kidney leucocytes than in FCA-injected ones. The level of lysozyme activity in the serum of fish 2 days after injection with mistletoe was also significantly higher than that in the serum of the control fish. The optimal concentration of mistletoe in inducing the highest serum lysozyme activity was revealed to 500${\mu}$g/200 g of fish. In phagocytic activity assay, mistletoe-sensitized eel kidney phagocytes captured more zymosan than did the control fish. Conclusion: Korean mistletoe appeared to be a good activator of the non-specific immune responses of eel.

Keywords

References

  1. Peddie S, Zou J, Secombes CJ: Immunostimulation in the rainbow trout (Oncorhynchus mykiss) following intraperitoneal administration of Ergosan. Veterinary Immunology Immunopathology 86;101-113, 2002 https://doi.org/10.1016/S0165-2427(02)00019-3
  2. Ellis AE: Immunity to bacteria in fish. Fish Shellfish Immunology 9;291-308, 1999 https://doi.org/10.1006/fsim.1998.0192
  3. Sarder MR, Thompson KD, Penman DJ, McAndrew BJ: Immune responses of nile tilapia (Oreochromis niloticus L.) clones: I. Non-specific responses. Dev Comp Immunol 25;37-46, 2001 https://doi.org/10.1016/S0145-305X(00)00040-9
  4. Iwama G, Nakanishi T: The fish immune system: in non-specific immune system: cellular defenses, Secombes CJ (eds.) pp. 63-68, Academic Press, California, USA, 1996
  5. Jewhurst VA, Todd D, Rowley HM, Walker IW, Weston JH, McLoughlin MF, Graham DA: Detection and antigenic characterization of salmonid alphavirus isolates from sera obtained from farmed Atlantic salmon, Salmo salar L., and farmed rainbow trout, Oncorhynchus mykiss (Walbaum). J Fish Dis 27;143-149, 2004 https://doi.org/10.1111/j.1365-2761.2004.00530.x
  6. Shen L, Stuge TB, Zhou H, Khayat M, Barker KS, Quiniou SM, Wilson M, Bengten E, Chinchar VG, Clem LW, Miller NW: Channel catfish cytotoxic cells: a mini-review. Dev Comp Immunol 26;141-149, 2002 https://doi.org/10.1016/S0145-305X(01)00056-8
  7. Ono H, Figueroa F, O'hUigin C, Klein J: Cloning of the beta 2-microglobulin gene in the zebrafish. Immunogenetics 38;1-10, 1993 https://doi.org/10.1007/BF00216384
  8. Jorgensen JB, Zou J, Johansen A, Secombes CJ: Immunostimulatory CpG oligodeoxynucleotides stimulate expression of IL-1beta and interferon-like cytokines in rainbow trout macrophages via a chloroquine-sensitive mechanism. Fish Shellfish Immunol 11;673-682, 2001 https://doi.org/10.1006/fsim.2001.0344
  9. Sakai M, Taniguchi K, Mamoto K, Ogawa H, Tabata M: Immunostimulant effects of nucleotide isolated from yeast RNA on carp, Cyprinus carpio L. J Fish Dis 24;433-438, 2001 https://doi.org/10.1046/j.1365-2761.2001.00314.x
  10. Mueller EA, Anderer FA: A viscum album oligosacchaaride activating human natural cytotoxicity is an interferon gamma inducer. Cancer Immunology Immunother 32;221-227, 1990 https://doi.org/10.1007/BF01741704
  11. Mannel DN, Becker H, Gundt A, Franz H: Induction of tumor necrosis factor expression by a lectin from Viscum album. Cancer Immunology Immunotherapy 33;177-182, 1991 https://doi.org/10.1007/BF01756139
  12. Kuttan GD, Vasudevan M, Kuttan R: Tumor reducing activity of an isolated active ingredient from mistletoe extract and its possible mechanism of action. J Exp Clin Cancer Res 11;7-12, 1992
  13. Mertzer GH, Franz H, Kindt A, Fahlbusch B, Sus J: The in vitro activity of lectin 1 from mistletoe (ML 1) and its isolated A and B chains in functions of macrophages and polymorphonuclear cells. Immunobiology 169;461-471, 1985 https://doi.org/10.1016/S0171-2985(85)80002-4
  14. Hajto T: Immunomodulatory effect of Iscador: a Viscum album preparation. Oncology 43(Suppl 1);51-61, 1986 https://doi.org/10.1159/000226420
  15. Lyu SY, Park SM, Choung BY, Park WB: Comparative study of Korean (Viscum album var. coloratum) and European mistletoe (Viscum album). Arch Pharm Res 23;592-598, 2000 https://doi.org/10.1007/BF02975247
  16. Yoon TJ, Yoo YC, Kang TB, Her E, Kim K, Azuma I, Kim JB: Cellular and humoral adjuvant activity of lectins isolated from Korean mistletoe (Viscum album colaratum). Int Immunopharmacol 1;881-889, 1990 https://doi.org/10.1016/S1567-5769(01)00024-8
  17. Secombes CJ, Chung S, Jeffries AH: Superoxide anion production by rainbow trout macrophages detected by the reduction of ferricytochrome C. Dev Comp Immunol 12; 201-206, 1988 https://doi.org/10.1016/0145-305X(88)90038-9
  18. Vasta GR, Ahmed H, Fink NE, Elola MT, Marsh AG, Snowden A, Odom EW: Animal lectins as self/non-self recognition molecules. Biochemical and genetic approaches to understanding their biological roles and evolution. Ann N Y Acad Sci 15;55-73, 1994
  19. Weis WI, Taylor ME, Drickamer K: The C-type lectin superfamily in the immune system. Immunol Rev 163;19-34, 1998 https://doi.org/10.1111/j.1600-065X.1998.tb01185.x
  20. Holmskov U, Malhotra R, Sim RB, Jensenius JC: Collectins: collagenous C-type lectins of the innate immune defense system. Immunol Today 15;67-74, 1994 https://doi.org/10.1016/0167-5699(94)90136-8
  21. Ni Y, Tizard I: Lectin-carbohydrate interaction in the immune system. Vet Immunol Immunopathol 55;205-223, 1993
  22. Lu J: Collectins: collectors of microorganisms for the innate immune system. Bioassay 19;509-518, 1997 https://doi.org/10.1002/bies.950190610
  23. Bliznakov EG, Adler AD: Nonlinear response of the reticuloendothelial system upon stimulation. Pathol Microbiol (Basel) 38;393-410, 1972
  24. Gialdroni-Grassi G, Grassi C: Bacterial products as immunomodulating agents. Int Arch Allergy Appl Immunol 76(Suppl 1);119-127, 1985 https://doi.org/10.1159/000233742
  25. Kenyon CJ, McKeever A, Oliver JA, Henderson IW: Control of renal and adrenocortical function by the renin-angio-tensin system in two euryhaline teleost fishes. Gen Comp Endocrinol 58;93-100, 1985 https://doi.org/10.1016/0016-6480(85)90140-6
  26. Anderson DP, Jeney G: Immunostimulants added to injected Aeromonas salmonicida bacterin enhance the defense mechanisms and protection in rainbow trout (Oncorhynchus mykiss). Vet Immunol Immunopathol 34;379-389, 1992 https://doi.org/10.1016/0165-2427(92)90177-R
  27. Siwicki AK, Cossarini-Dunier M, Studnicka M, Demael A: In vivo effect of the organophosphorus insecticide trichlorphon on immune response of carp (Cyprinus carpio). II. Effect of high doses of trichlorphon on nonspecific immune response. Ecotoxicol Environ Saf 19;99-105, 1990 https://doi.org/10.1016/0147-6513(90)90084-I