Journal of Mushroom (한국버섯학회지)
- Volume 6 Issue 1
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- Pages.20-26
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- 2008
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- 1738-0294(pISSN)
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- 2288-8853(eISSN)
Optimization of artificial cultivation of Tremella fuciformis in closed culture bottle
흰목이버섯 대량생산을 위한 용기내 재배 최적화 연구
- Choi, Sung Woo (Dept. of Bioengineering and Genetic Engineering, College of Natural Sciences, The University of Suwon) ;
- Chang, Hyun-You (Dept. of Mushroom Science, Korea National Agricultural College) ;
- Yoon, Jeong Weon (Dept. of Bioengineering and Genetic Engineering, College of Natural Sciences, The University of Suwon) ;
- Lee, Chan (Dept. of Food Science and Technology, Chung-Ang University)
- Published : 2008.03.31
Abstract
The stromatal forms of T. fuciformis and the mycelia of Hypoxylon sp. were collected. The DNA sequence in the ITS region of the 5.8S ribosomal genes of isolated strain KG103 was very similar to that of T. fuciformis AF042409 with a homology of over 98% in the EMBL/GenBank database through BLAST searching. A second isolate, No KG201, one of the symbiotic strains for cultivating T. fuciformis also exhibited high homology with Annulohhypoxylon stygium AJ390406. Potato Dextrose Medium exhibited the best mycelial growth of 14 mm/14 days and 85 mm/14 days for T. fuciformis and its symbiotic fungi, respectively. Optimum culture conditions for the micelial growth were pH 5 at
흰목이버섯 균주와 공생균을 수집하고 ITS 5.8S rDNA sequencing을 하여 유전자 서열을 분석하였다. Gene Bank Data homology search 결과 분리된 균의 rDNA 서열이 이 Tremella fuciformis AF042409의 rDNA 서열과 99% 일치하는 것으로 확인되었다. 그리고 함께 분리된 공생균은 같은 방법으로 Annulohhypoxylon stygium 으로 확인하였다. 분리된 T. fuciformis KG 103과 A. stygium KG 201 균주는 PD배지에서 각각 14 mm/14 days과 85 mm/14 days의 균사생육을 나타내었다. T. fuciformis KG 103 균주의 생육최적온도는