International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
권호 표지

Characterization of Mamestra brassicae Nucleopolyhedrovirus (MabrNPV)-K1 Isolated in Korea

  • Lee, Jae-Kyung (School of Applied Life Science & Environment, College of Agriculture Life & Environment Science, Chungbuk National University) ;
  • Shin, Tae-Young (School of Applied Life Science & Environment, College of Agriculture Life & Environment Science, Chungbuk National University) ;
  • Bae, Sung-Min (School of Applied Life Science & Environment, College of Agriculture Life & Environment Science, Chungbuk National University) ;
  • Choi, Jae-Bang (School of Applied Life Science & Environment, College of Agriculture Life & Environment Science, Chungbuk National University) ;
  • Oh, Jeong-Mi (School of Applied Life Science & Environment, College of Agriculture Life & Environment Science, Chungbuk National University) ;
  • Koo, Hyun-Na (School of Applied Life Science & Environment, College of Agriculture Life & Environment Science, Chungbuk National University) ;
  • Kim, Ju-Il (Lab of Crop Protection Research, Environment Management Division, National Institute of Highland Agriculture, RDA) ;
  • Kwon, Min (Lab of Crop Protection Research, Environment Management Division, National Institute of Highland Agriculture, RDA) ;
  • Woo, Soo-Dong (School of Applied Life Science & Environment, College of Agriculture Life & Environment Science, Chungbuk National University)
  • Published : 2008.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to investigate the characteristics of Mamestra brassicae nucleopolyhedrovirus (MabrNPV)-K1 isolated in Korea. Polyhedra of MabrNPV-K1 showed irregular appearance in shape with the average diameter $1.8{\mu}m$. MabrNPV-K1 contained a number of nucleocapsids within a viral envelope embedded in polyhedron. The polyhedrin of MabrNPV-K1 was composed of single polypeptide with a M.W. of approximate 31 kDa which is identical to the commercialized MabrNPV, Mamestrin, as a biological control agent. The nucleotide and amino acid sequences within the coding region of MabrNPV-K1 polyhedrin shared 99.0% similarity with the polyhedrin gene from previous reported MabrNPVs. The median lethal concentrations ($LC_{50}$) of MabrNPV-K1 and Mamestrin to M. brassicae larvae were $3.9{\times}10^3$ PIBs/larva and $6.0{\times}10^4$ PIBs/larva, respectively. Mortality of the MabrNPV-K1 against to the third instars larvae was 15 times higher than that of the Mamestrin. The median lethal times ($LT_{50})$ of MabrNPV-K1 by the concentration of polyhedra were lower ($4.4{\sim}6.1$ days) than those of Mamestrin ($4.1{\sim}8.6$ days). These results suggest that a local strain MabrNPV-K1 has high pathogenicity to M. brassicae and may be useful for the development of biological control agent to control this.

Keywords

References

  1. Akutsu, K. (1972) The use of viruses for the control of vegetable pests. Plant Prot. 26, 19-23
  2. Aruga, H., N. Yoshitake, H. Watanabe and T. Hukuhara (1960) Studies on nuclear polyhedrosis and their induction in some Lepidoptera. Jpn J. Appl. Entomol. Zool. 4, 51-56 https://doi.org/10.1303/jjaez.4.51
  3. Brown, D. A., H. F. Evans, C. J. Allen and D. C. Kelly (1981) Biological and biochemical investigations on five European isolates of Mamestra brassicae nuclear polyhedrosis virus. Arch. Virol. 69, 209-217 https://doi.org/10.1007/BF01317336
  4. Cameron, I. R. and R. D. Possee (1989) Conservation of poly-hedrin gene promoter function between Autographa californica and Mamestra brassicae nuclear polyhedrosis viruses. Virus Res. 12(3), 99-83
  5. Clarke, E. E., M. Tristem, J. S. Cory and D. R. O'Reilly (1996) Characterization of the ecdysteroid UDP-glucosyltransferase gene from Mamestra brassicae nucleopolyhedrovirus. J. Gen. Virol. 77, 2865-2871 https://doi.org/10.1099/0022-1317-77-11-2865
  6. Erlandson, M. A. (1990) Biological and biochemical comparison of Mamestra configurata and Mamestra brassicae nuclear polyhedrosis virus isolates pathogenic for the bertha armyworm, Mamestra configurata (Lepidoptera: Noctuidae). J. Invertebr. Pathol. 56, 47-56 https://doi.org/10.1016/0022-2011(90)90143-T
  7. Evans, H. F. and G. P. Allaway (1983) Dynamic of baculovirus growth and dispersal in Mamestra brassicae L. (Lepidoptera: Noctuidae) larval populations introduced into small cabbage plots. Appl. Environ. Microbiol. 45, 493-501
  8. Finney, D. J. (1971) Probit analysis. 3rd (ed.), pp. 33, Cambridge University Press, Cambridge
  9. Kelly, D. C. and D. A. Brown (1980) Biochemical and biophysical properties of a Mamestra brassicae multiple enveloped nuclear polyhedrosis virus. Arch. Virol. 66, 133-141 https://doi.org/10.1007/BF01314981
  10. Laemmli, U. K. (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227, 680-685 https://doi.org/10.1038/227680a0
  11. Maeda, S. (1994) Expression of foreign genes in insect cells using baculovirus vectors; in Insect cell biotechnology. Maramorosch, K. and A. McIntosh (eds.), pp. 1-31, Boca Raton, CRC Press, FL
  12. Miller, L. K. (1988) Baculoviruses as gene expression vectors. Annu. Rev. Microbol 42, 177-199 https://doi.org/10.1146/annurev.mi.42.100188.001141
  13. Murphy, F. A., C. M. Fauquet, D. H. L. Bisop, S. A. Ghabrial, A. W. Jarvis, G. P. Martinelli, M. A. Mayo and M. D. Summers (1995) Virus taxonomy. Sixth report of the international committee on taxonomy of viruses. Arch. Virol. Suppl. 10, 85-86
  14. Okada, M. (1977) Studies on the utilization and mass production of Spodoptera litura nuclear polyhedrosis virus for control of the tobacco cutworm, Spodoptera litura Fabricius. Agric. Exp. Stn. Sere. 12, 1-66
  15. Rovesti, L., N. E. Crook and D. Winstanley (2000) Biological and biochemical relationships between the ncleopolyhedoviruses of Mamestra brassicae and Heliothis armigera. J. Invertebr. Pathol. 75, 2-8 https://doi.org/10.1006/jipa.1999.4903
  16. Smith, G. E., M. D. Summers and M. D. Fraser (1983) Production of human $\beta$-interferon on insect cells infected with a baculovirus expression vector. Mol. Cell Biol. 3, 2156-2165 https://doi.org/10.1128/MCB.3.12.2156
  17. Valk, J. M. and A. Groner (1980) Identification of two nuclear polyhedrosis viruses from the cabbage moth, Mamestra brassicae (Lepidoptera: Noctuidae). J. Invertebr Pathol. 35, 269-278 https://doi.org/10.1016/0022-2011(80)90162-7
  18. Woo, S. D., K. H. Cho, B. R. Jin, K. S. Boo and S. K. Kang (2000) Expression of the gene encoding firefly luciferase using Bombyx mori nucleopolyhedrovirus vector. Int. J. Ind. Entomol. 1, 53-58