Molecules and Cells

  • 제25권4호
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  • Pages.559-565
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  • 2008
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
권호 표지

Isolation of CONSTANS as a TGA4/OBF4 Interacting Protein

  • Song, Young Hun (Environmental Biotechnology National Core Research Center, Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology Biotechnology Research Center, Gyeongsang National University) ;
  • Song, Na Young (Environmental Biotechnology National Core Research Center, Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology Biotechnology Research Center, Gyeongsang National University) ;
  • Shin, Su Young (Environmental Biotechnology National Core Research Center, Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology Biotechnology Research Center, Gyeongsang National University) ;
  • Kim, Hye Jin (Environmental Biotechnology National Core Research Center, Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology Biotechnology Research Center, Gyeongsang National University) ;
  • Yun, Dae-Jin (Environmental Biotechnology National Core Research Center, Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology Biotechnology Research Center, Gyeongsang National University) ;
  • Lim, Chae Oh (Environmental Biotechnology National Core Research Center, Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology Biotechnology Research Center, Gyeongsang National University) ;
  • Lee, Sang Yeol (Environmental Biotechnology National Core Research Center, Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology Biotechnology Research Center, Gyeongsang National University) ;
  • Kang, Kyu Young (Environmental Biotechnology National Core Research Center, Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology Biotechnology Research Center, Gyeongsang National University) ;
  • Hong, Jong Chan (Environmental Biotechnology National Core Research Center, Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology Biotechnology Research Center, Gyeongsang National University)
  • 투고 : 2007.11.06
  • 심사 : 2007.11.27
  • 발행 : 2008.06.30
⟨ 이전 논문 다음 논문 ⟩

초록

Members of the TGA family of basic domain/leucine zipper transcription factors regulate defense genes through physical interaction with NON-EXPRESSOR OF PR1 (NPR1). Of the seven TGA family members, TGA4/octopine synthase (ocs)-element-binding factor 4 (OBF4) is the least understood. Here we present evidence for a novel function of OBF4 as a regulator of flowering. We identified CONSTANS (CO), a positive regulator of floral induction, as an OBF4-interacting protein, in a yeast two-hybrid library screen. OBF4 interacts with the B-box region of CO. The abundance of OBF4 mRNA cycles with a 24 h rhythm under both long-day (LD) and short-day (SD) conditions, with significantly higher levels during the night than during the day. Electrophoretic mobility shift assays revealed that OBF4 binds to the promoter of the FLOWERING LOCUS T (FT) gene, a direct target of CO. We also found that, like CO and FT, an OBF4:GUS construct was prominently expressed in the vascular tissues of leaf, indicating that OBF4 can regulate FT expression through the formation of a protein complex with CO. Taken together, our results suggest that OBF4 may act as a link between defense responses and flowering.

키워드

과제정보

연구 과제 주관 기관 : Korea Science and Engineering Foundation

참고문헌

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