Effect of Jasmonic Acid on the Root Growth and the Eleutheroside Accumulation in the Adventitious Root Culture of Eleutherococcus senticosus

가시오갈피나무 부정근(不定根) 배양(培養)에서 부정근(不定根)의 생장(生長)과 Eleutheroside유(類)의 생산(生産)에 미치는 Jasmonic acid처리(處理)의 영향

This study was carried out to investigate the dose-dependent effect of jasmonic acid on both the adventitious root growth and the accumulation of various eleutherosides in the Eleutherococcus senticosus bioreactor culture. The highest biomass production (4.6 g DW/L) was observed in the absence of jasmonic acid and the root growth was significantly decreased by increasing the jasmonic acid concentration. However, jasmonic acid stimulated the production of both eleutheroside B, E and $E_1$. The highest level of eleutheroside B and E ($476.3{\mu}g/g\;DW$ and $676.0{\mu}g/g\;DW$) was obtained by 0.5 mg/L jasmonic acid treatment, while eleutheroside $E_1$ was accumulated at the highest level by 0.01 mg/L jasmonic acid treatment. The highest content of total eleutheroside was $2468.6{\mu}g/L$ when 0.01 mg/L jasmonic acid was applied. In addition, when the adventitious roots were cultured with 0.01 mg/L jasmonic acid, the highest levels of eleutheroside B, E and $E_1$ were observed at the 6th, 8th and 4th day of culture, respectively.

생물반응기를 이용한 가시오갈피 부정근 배양시에 jasmonic acid를 농도별 (0, 0.01, 0.05, 0.1, 03, 0.5 mg/L)로 처리하여 부정근의 생장과 eleutheroside류 생산과의 관계를 조사하였다. Jasmonic acid 처리농도별 부정근의 생장은 무처리구에서 4.6 g Dry weight (DW)/L로 가장 높았으며 jasmonic acid 농도가 증가할수록 부정근 생장은 감소하였다. 그러나 eleutheroside B와 E의 함량은 jasmonic acid 농도가 높을수록 증가하여 0.5 mg/L 처리구에서 각각 $476.3{\mu}g/g\;DW$, $676.0{\mu}g/g\;DW$로 생산량이 가장 많았다. 반면에 eleutheroside $E_1$은 0.01 mg/L 처리구에서 $46.1{\mu}g/g\;DW$로 생산량이 가장 많았다. 배지 1L당 eleutheroside류의 총생산량은 0.01 mg/L 처리구에서 $2468.6{\mu}g/L$를 생산하여 가장 우수하였다. 0.1 mg/L의 jasmonic acid 처리 후 10일간 eleutheroside류의 함량을 조사한 결과 eleutheroside B는 jasmonic acid처리 후 6일째, eleutheroside E는 처리 후 8일째, eleutheroside $E_1$은 처리 후 4일째 각각 가장 많은 생산량을 보여 주었다.