Journal of Dental Rehabilitation and Applied Science (구강회복응용과학지)

Korean Academy of Stomatognathic Function and Occlusion (대한턱관절교합학회)
권호 표지

Rheological Properties of Calcium Phosphate Cement Mixed with 2 Kinds of Setting Solution

2종의 경화액과 혼합된 calcium phosphate cement의 유변학적 성질에 관한 연구

  • Chang, Seok-Woo (Department of Conservative Dentistry, The institute of Oral Health Science, Samsung Medical Center, Sungkyunkwan University School of Medicine) ;
  • Kwon, Ho-Beom (Department of Prosthodontics, The institute of Oral Health Science, Samsung Medical Center, Sungkyunkwan University School of Medicine) ;
  • Yoo, Hyun-Mi (Department of Conservative Dentistry, The institute of Oral Health Science, Samsung Medical Center, Sungkyunkwan University School of Medicine) ;
  • Park, Dong-Sung (Department of Conservative Dentistry, The institute of Oral Health Science, Samsung Medical Center, Sungkyunkwan University School of Medicine) ;
  • Oh, Tae-Seok (Department of Conservative Dentistry, The institute of Oral Health Science, Samsung Medical Center, Sungkyunkwan University School of Medicine) ;
  • Bae, Kwang-Shik (Department of Conservative Dentistry, School of Dentistry, Seoul National University)
  • 장석우 (성균관대학교 의과대학 삼성서울병원 치과진료부 보존과) ;
  • 권호범 (성균관대학교 의과대학 삼성서울병원 치과진료부 보철과) ;
  • 유현미 (성균관대학교 의과대학 삼성서울병원 치과진료부 보존과) ;
  • 박동성 (성균관대학교 의과대학 삼성서울병원 치과진료부 보존과) ;
  • 오태석 (성균관대학교 의과대학 삼성서울병원 치과진료부 보존과) ;
  • 배광식 (서울대학교 치의학전문대학원 보존학 교실)
  • Received : 2008.05.15
  • Accepted : 2008.09.25
  • Published : 2008.09.30
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Abstract

Calcium phosphate cement (CPC) has been used as bone substitute successfully due to good biocompatibility and osteoconductivity. One of the important mechanical characteristics of CPC is flowablility, which can be evaluated by measuring rheological parameters. However, there have been few studies that measured rheological properties of CPC. The purpose of this study was to evaluate the rheological properties of CPC paste mixed with 2 kinds of setting solutions, 2% hydroxyprophyl methylcellulose (HPMC) and 35% polyacrylic acid (PAA). The CPC used was dicalcium phosphate dihydrate (DCPD). Rheological properties of CPC paste were measured using rheometer. The statistical analysis was carried out with Mann-whitney test with Bonferronis collection. CPC with both setting solutions showed shear thinning behavior. CPC with 2% HPMC showed signigicantly higher complex viscosity than CPC with 35% PAA(p<0.05).

Calcium phosphate cement (CPC)는 우수한 생체 친화성을 바탕으로 치과 및 의과 쪽에서 성공적인 bone substitute로 사용되어 왔다. 긴 경화시간 및 washout tendency 등 CPC의 단점을 개선하기 위해 다양한 종류의 경화액 및 첨가제등에 대한 연구가 이루어졌다. 그러나 첨가제의 종류에 따른 CPC paste의 점탄성을 정량적으로 비교한 연구는 많지 않다. 이 연구에서는 2% hydroxyprophyl methylcellulose (HPMC)와 35% polyacrylic acid (PAA)의 두 가지 경화액과 혼합된 CPC의 유변학적 성질을 관찰하고 비교하고자 하였다. Dicalcium phosphate dihydrate (DCPD)를 2% HPMC 및 35% PAA와 각각 1:1의 분액비로 30초간 섞은 후 cone and plate geometry를 가지는 rheometer를 사용하여 frequency sweep test와 time sweep test를 통해 shear storage modulus (G'), shear loss modulus (G''), 그리고 complex viscosity (${\eta}^*$)를 측정하였다. 2% HPMC군과 35% PAA군의 complex viscostiy의 차이를 Mann-whitney test with Bonferroni's collection을 사용하여 분석하였다. 실험결과 2% HPMC 및 35% PAA 군 모두에서 shear thinning과 yield behavior등 pseudoplastic property를 보였으며 complex viscosity는 HPMC 군에서 PAA 군보다 통계적으로 유의성 있게 높았다. (p<0.05).

Keywords

References

  1. Wang X, Ye J, Wang Y, Chen L. Self-setting properties of a beta-dicalcium silicate reinforced calcium phosphate cement. J Biomed Mater Res B Appl Biomater 2007;82:93-9
  2. Ginebra MP, Fernandez E, De Maeyer EA, Verbeeck RM, Boltong MG, Ginebra J, et al. Setting reaction and hardening of an apatitic calcium phosphate cement. J Dent Res 1997;76:905-12 https://doi.org/10.1177/00220345970760041201
  3. Comuzzi L, Ooms E, Jansen JA. Injectable calcium phosphate cement as a filler for bone defects around oral implants: an experimental study in goats. Clin Oral Implants Res 2002;13:304-11 https://doi.org/10.1034/j.1600-0501.2002.130311.x
  4. Shirakata Y, Yoshimoto T, Goto H, Yonamine Y, Kadomatsu H, Miyamoto M, et al. Favorable periodontal healing of 1-wall infrabony defects after application of calcium phosphate cement wall alone or in combination with enamel matrix derivative: a pilot study with canine mandibles. J Periodontol 2007;78:889-98 https://doi.org/10.1902/jop.2007.060353
  5. Sugawara A, Fujikawa K, Kusama K, Nishiyama M, Murai S, Takagi S, et al. Histopathologic reaction of a calcium phosphate cement for alveolar ridge augmentation. J Biomed Mater Res 2002;61:47-52 https://doi.org/10.1002/jbm.10010
  6. Xu HH, Takagi S, Sun L, Hussain L, Chow LC, Guthrie WF, et al. Development of a nonrigid, durable calcium phosphate cement for use in periodontal bone repair. J Am Dent Assoc 2006;137: 1131-8 https://doi.org/10.14219/jada.archive.2006.0353
  7. Yang SE, Baek SH, Lee W, Kum KY, Bae KS. In vitro evaluation of the sealing ability of newly developed calcium phosphate-based root canal sealer. J Endod 2007;33:978-81 https://doi.org/10.1016/j.joen.2006.07.023
  8. Schirrmeister JF, Kielbassa AM. Coronal leakage of calcium phosphate-based root canal sealers compared with usual sealers. Schweiz Monatsschr Zahnmed 2006;116:224-8
  9. Ambard AJ, Mueninghoff L. Calcium phosphate cement: review of mechanical and biological properties. J Prosthodont 2006;15:321-8 https://doi.org/10.1111/j.1532-849X.2006.00129.x
  10. Burguera EF, Xu HH, Weir MD. Injectable and rapid-setting calcium phosphate bone cement with dicalcium phosphate dihydrate. J Biomed Mater Res B Appl Biomater 2006;77:126-34
  11. Wang X, Ye J, Wang H. Effects of additives on the rheological properties and injectability of a calcium phosphate bone substitute material. J Biomed Mater Res B Appl Biomater 2006;78:259-64
  12. Bohner M, Baroud G. Injectability of calcium phosphate pastes. Biomaterials 2005;26:1553-63 https://doi.org/10.1016/j.biomaterials.2004.05.010
  13. Baroud G, Matsushita C, Samara M, Beckman L, Steffen T. Influence of oscillatory mixing on the injectability of three acrylic and two calcium- phosphate bone cements for vertebroplasty. J Biomed Mater Res B Appl Biomater 2004;68:105-11
  14. Jack V, Buchanan FJ, Dunne NJ. Particle attrition of alpha-tricalcium phosphate: effect on mechanical, handling, and injectability properties of calcium phosphate cements. Proc Inst Mech Eng [H] 2008;222:19-28
  15. Alves HL, Dos Santos LA, Bergmann CP. Injectability evaluation of tricalcium phosphate bone cement. J Mater Sci Mater Med 2008;19:2241-6 https://doi.org/10.1007/s10856-007-3329-6
  16. Baroud G, Bohner M, Heini P, Steffen T. Injection biomechanics of bone cements used in vertebroplasty. Biomed Mater Eng 2004;14:487-504
  17. Cherng A, Takagi S, Chow LC. Effects of hydroxypropyl methylcellulose and other gelling agents on the handling properties of calcium phosphate cement. J Biomed Mater Res 1997;35: 273-7 https://doi.org/10.1002/(SICI)1097-4636(19970605)35:3<273::AID-JBM1>3.0.CO;2-E
  18. Liu C, Shao H, Chen F, Zheng H. Rheological properties of concentrated aqueous injectable calcium phosphate cement slurry. Biomaterials 2006;27: 5003-13 https://doi.org/10.1016/j.biomaterials.2006.05.043
  19. Wang X, Chen L, Xiang H, Ye J. Influence of anti-washout agents on the rheological properties and injectability of a calcium phosphate cement. J Biomed Mater Res B Appl Biomater 2007;81:410-8
  20. Burguera EF, Guitian F, Chow LC. A water setting tetracalcium phosphate-dicalcium phosphate dihydrate cement. J Biomed Mater Res A 2004;71:275-82
  21. Leroux L, Hatim Z, Freche M, Lacout JL. Effects of various adjuvants (lactic acid, glycerol, and chitosan) on the injectability of a calcium phosphate cement. Bone 1999;25:31S-4S https://doi.org/10.1016/S8756-3282(99)00130-1