Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Comparison of antigenicity of Edwardsiella tarda isolates in loach(Misgurnus mizloepis)

미꾸라지에서의 Edwardsiella tarda isolates의 항원성 비교

  • Lee, Young (National Fisheries Research and Development Institute, Goseong Fisheries Office) ;
  • Jun, Lyu-Jin (Department of Aquatic Life Medicine, College of Fisheries Science, Pukyong National University) ;
  • Kim, Myoung-Suk (National Fisheries Research and Development Institute, Pathology Team) ;
  • Park, Kyung-Hyun (National Fisheries Research and Development Institute, South Sea Fisheries Research Institute) ;
  • Jeong, Hyun-Do (Department of Aquatic Life Medicine, College of Fisheries Science, Pukyong National University)
  • 이영 (국립수산과학원 고성수산사무소) ;
  • 전려진 (부경대학교 수산생명의학과) ;
  • 김명석 (국립수산과학원 병리연구팀) ;
  • 박경현 (국립수산과학원 남해수산연구소) ;
  • 정현도 (부경대학교 수산생명의학과)
  • Published : 20081200
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Abstract

We compared the pathogenicity and antigenicity of two different Edwardsiella tarda (E. tarda) strains KFE and Edk-2 isolated in Korea and Japan respectively. In the pathogenicity analysis with challenge test against loach, E. tarda KFE isolate showed stronger pathogenicity compared to that of E. tarda Edk-2 isolate. The differences were also confirmed by the comparison of OMP (outer membrane protein) in SDSPAGE which showed three major bands, 41kDa, 37kDa and 30kDa, for E. tarda KFE isolates and two major bands, 41kDa and 30kDa, for E. tarda Edk-2 isolates. On the base of these results, we tried to determine the differences of antigenicities of these two isolates in loach which is one of the important species in freshwater aquaculture in Korea. Numbers of specific antibody secreting cells (SASC), appeared to be higher in loach immunized with FKC of E. tarda Edk-2 than loach immunized with FKC of E. tarda KFE. ELISPOT-assay for the comparison of antigenicity showed relatively high percentage of cross-reaction and implied the presence of some common epitopes in the antigens of these two E. tarda isolates.

Keywords

References

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