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생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Acrolein, the toxic endogenous aldehyde, induces neurofilament-L aggregation

  • 발행 : 2008.09.30
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초록

Acrolein is a highly reactive by product of lipid peroxidation and individuals with neurodegenerative disorders have been shown to contain elevated concentrations of this molecule in the brain. In the present study, we examined the pattern of neurofilament-L (NF-L) modification elicited by acrolein. When NF-L was incubated with acrolein, protein aggregation occurred in a acrolein concentration-dependent manner. Exposure of NF-L to acrolein also led to the generation of protein carbonyl compounds. Through the addition of free radical scavengers we observed a significant decrease in acrolein-mediated NF-L aggregation. These results indicate that free radicals may be involved in the modification of NF-L by acrolein. In addition, dityrosine crosslink formation was observed in acrolein-mediated NF-L aggregates and these aggregates displayed thioflavin T reactivity, reminiscent of amyloid. This study suggests that acrolein-mediated NF-L aggregation might be closely related to oxidative reactions, thus these reactions may play a critical role in neuro-degenerative diseases.

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피인용 문헌

  1. The Nervous System Cytoskeleton under Oxidative Stress vol.1, pp.1, 2013, https://doi.org/10.3390/diseases1010036
  2. Molecular Mechanisms of Acrolein Toxicity: Relevance to Human Disease vol.143, pp.2, 2015, https://doi.org/10.1093/toxsci/kfu233
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  6. Free-radicals and advanced chemistries involved in cell membrane organization influence oxygen diffusion and pathology treatment vol.4, pp.2, 2017, https://doi.org/10.3934/biophy.2017.2.240
  7. Acrolein acts as a neurotoxin in the nigrostriatal dopaminergic system of rat: involvement of α-synuclein aggregation and programmed cell death vol.7, 2017, https://doi.org/10.1038/srep45741
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