Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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The serodiagnosis of a lymphocystis disease virus infection using an antibody raised against a recombinant major capsid protein

  • Seo, Ja-Young (Biotherapy Human Resources Center, College of Veterinary Medicine, Chonnam National University) ;
  • Kang, Bong-Jo (The Jeju Special Self-Governing Province Fisheries Resources Research Institute) ;
  • Oh, Hyoung-Jong (Jokwang Bio-immunity Center) ;
  • Lee, Jae-Il (Biotherapy Human Resources Center, College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Tae-Jung (The Jeju Special Self-Governing Province Fisheries Resources Research Institute)
  • Published : 20081200
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Abstract

Lymphocystis is a viral disease of fish primarily in marine and brackishwaters. Here we report the cloning, expression, and the serological applications of the lymphocystis disease virus (LCDV) major capsid protein (MCP). The MCP gene was amplified by PCR from the genomic DNA of LCDV isolated from Schlegel's black rockfish, Sebastes schlegeli, and expressed in E. coli. Mouse antisera raised against the purified recombinant MCP (rMCP) reacted with the viral MCP in an immunofluorescence assay, indicating that this rMCP would be useful for serological studies of field samples.

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References

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