Journal of Korean Society of Occupational and Environmental Hygiene (한국산업보건학회지)

Korean Industrial Hygiene Association (한국산업보건학회)
권호 표지

A Study on thinner's Physicochemical property and its effect on genital organ of rat

시너(thinner)의 물리화학적 특성과 랫트의 생식기에 미치는 영향 연구

  • Kim, Hyeon-Yeong (Chemical Safety&Health Research Center, Occupational Safety&Health Research Institute) ;
  • Lee, Sung-Bae (Chemical Safety&Health Research Center, Occupational Safety&Health Research Institute) ;
  • Han, Jeong-Hee (Chemical Safety&Health Research Center, Occupational Safety&Health Research Institute) ;
  • Kim, Tae-Gyun (Chemical Safety&Health Research Center, Occupational Safety&Health Research Institute) ;
  • Lee, Jung-Suk (Chemical Safety&Health Research Center, Occupational Safety&Health Research Institute) ;
  • Kang, Min-Gu (Chemical Safety&Health Research Center, Occupational Safety&Health Research Institute) ;
  • Lee, Chae-Kwan (Institute of Industrial Medicine, Department of Occupational and Environmental Medicine, Inje University)
  • 김현영 (산업안전보건연구원 화학물질안전보건센터) ;
  • 이성배 (산업안전보건연구원 화학물질안전보건센터) ;
  • 한정희 (산업안전보건연구원 화학물질안전보건센터) ;
  • 김태균 (산업안전보건연구원 화학물질안전보건센터) ;
  • 이정석 (산업안전보건연구원 화학물질안전보건센터) ;
  • 강민구 (산업안전보건연구원 화학물질안전보건센터) ;
  • 이채관 (인제대학교 산업의학연구소)
  • Received : 2008.06.18
  • Accepted : 2008.09.04
  • Published : 2008.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this study is to confirm the physicochemical property and hazard of thinner (012), which is a diluent of enamel paint used for floor coating for waterproofing and oil painting for the outer wall. The literatures of physicochemical property and hazard of thinner were surveyed and its physicochemical property were evaluated. And then, the inhalation toxicity of thinner affecting the central nervous system and reproductive organs in rats were examined by subchronic (6 h./day. 5 days/ week for 13 weeks) inhalation test. 1) According to the 13-week subchronic inhalation test, there were no significant changes in clinical test and body weight. However, a significant evidence of toxicity was observed in the hematological test and organ weight such as heart, kidney, liver and brain (p<0.01) in the 200 ppm and 1,000 ppm exposure groups in a dose response manner. In the histopathology analysis, there were no significant evidence of toxicity. Therefore, thinner was not classified as an organ targeted toxic agent. In case of Harmfulness, it could be classified as a chronic toxic agent 3($500 ppm/4hr, rat). 2) The reproductive toxicity such as extension of the period of estrous cycle, reduction of serum estradiol concentration and increase of frequency of the abnormal sperm was observed in the 1,000 ppm exposed animals. 3) The result of the physicochemical property of the test material showed that the specific gravity was 0.793, boiling point $155.8^{\circ}C$, steam pressure 2.1 kPa, ignition point $34.5^{\circ}C$, and spontaneous ignition point $280^{\circ}C$. The endothermic and exothermic values were 371.4 J/g and 159.1 J/g. respectively. The explosion limit was 214 mg/l. These data showed that thinner could be classified as an explosion agent level 1.2 and ignitive liquid agent 3 ($23-60^{\circ}C$) according to the notification No. 2008-1 of the Labor Ministry, "Classifying Standard of Chemical Materials."

Keywords

References

  1. 강진석, 남기택, 박인승, 안병우, 양기화 등. 독성병리학 도감. 청구문회사; 2001. (80-81쪽)
  2. 김현중. 친환경 건축자재 및 석면기술. 대한석면관리협회. 2007;10-12
  3. 노동부. 화학물질의 분류.표시 및 물질안전보건자료에 관한기준(노동부고시 제2008-1호), 노동부. 2008
  4. 백남원, 윤충식, 조경이, 정회명. 우리나라에서 사용되고 있는 일부 시너의 구성성분에 관한 연구, 한국산업위생학회지 1998;8(1):105-114
  5. 산업안전보건연구원. 직업병심의사례. 1996-2006
  6. 한국산업안전공단. 물질안전보건자료(MSDS), 안전보건정보서비스 (KOSHA NET), http://www.kosha.net/shdb/msds/main.jsp. 2006
  7. Baydas G, Ozveren F, Tuzcu M, Yasar A. Effects of thinner exposure on the expression pattern of neural cell adhesion molecules, level of lipid peroxidation in the brain and cognitive function in rats. Eur J Pharmacol. 2005 Apr 11;512(2-3):181-7 https://doi.org/10.1016/j.ejphar.2005.02.038
  8. Boorman GA, Eustis SL, Elwell MR, Montgomery CA Jr, Mackenzie WF. 1990: Pathology of the Fischer rat. Reference and atlas. San Diego, CA: Academic Press
  9. Burmistrov SO, Arutyunyan AV, Stepanov MG, Oparina TI, Prokopenko VM. Effect of chronic inhalation of toluene and dioxane on activity of free radical processes in rat ovaries and brain. Bull Exp Biol Med. 2001 Sep;132(3):832-6 https://doi.org/10.1023/A:1013106315140
  10. Correa et al. Ethylene glycol ether and spontaneus abortion and subfertilty. Am J of Epidem. 1996:143:707-717 https://doi.org/10.1093/oxfordjournals.aje.a008804
  11. Davis BJ, Travlos G and McShane T. (2001): Reproductive endocrinology and toxicological pathology over the life span of the female rodent. Toxicol. Pathol., 29, 77-83 https://doi.org/10.1080/019262301301418874
  12. Goldman JM and Murr AS. (2003): Dibromoacetic acid-induced elevations in circulating estradiol: Effects in both cycling and ovariectomized/steroid-primed female rats. Reprod. Toxicol., 17, 585-592 https://doi.org/10.1016/S0890-6238(03)00068-6
  13. Greaves P. 1990: Histopathology of preclinical studies: Interpretation and relevance in drug evaluation. New York: Elsevier
  14. Haschek WM, Rousseaux CG. 1998: Fundamentals of toxicologic pathology. San Diego, CA: Academic Press
  15. Kim HY, Lee SB, Chung YH, LimCH, Yu IJ, Park SC, Shin JY, Kim SH, Shin DH, Kim JC. 2006: Evaluation of subchronic inhalation toxicity of dimethyl disulfide in rats. Inhal Toxicol, 18:395-403 https://doi.org/10.1080/08958370500516291
  16. Ng TP, Foo SC, Yoong T. Menstrual function in workers exposed to toluene. Br J Ind Med. 1992;49:799-803
  17. OECD. OECD Guideline for Testing of Chemicals, TG 413, Subchronic Inhalation Toxicity: 90-day Study. Paris, France; 1996
  18. OECD. OECD Guideline for Testing of Chemicals Draft Proposal for a New Guideline : Acute Inhalation Toxicity - Fixed Concentration Procedure; 2004
  19. Panova Z. Menstrual and reproductive functions and gynaecological morbidity in women occupational exposed to petrol[translation]. Letopisi Na Higienno-Epidemiologicnata Sluzba 1976;20:53-56
  20. Syrovadko ON, Skornin VF, Pronkova YeN, et al. Influence of working conditions on the state of health and certain specific functions of women working with white spirit. Gig Tr Prof Zabol 1973;6:5-8
  21. Winder C, Ng S. The problem of variable ingredients and concentration of solvent thinners. Am Ind Hyg assoc J. 56:1225-1228,1995 https://doi.org/10.1080/15428119591016250
  22. Yamada K. Influence of lacquer thinner and some organic solvents on reproductive and accessory reproductive organs in the male rat. Biol Pharm Bull. 1993 Apr;16(4):425-7 https://doi.org/10.1248/bpb.16.425
  23. Yilmaz B, Canpolat S, Sandal S, Akpolat N, Kutlu S, Ilhan N, Kelestimur H. Paint thinner exposure inhibits testosterone synthesis and secretion in a reversible manner in the rat. Reprod Toxicol. 2006 Nov;22(4):791-6 https://doi.org/10.1016/j.reprotox.2006.08.002
  24. Yilmaz B, Kutlu S, Canpolat S, Sandal S, Ayar A, Mogulkoc R, Kelestimur H. Effects of paint thinner exposure on serum LH, FSH and testosterone levels and hypothalamic catecholamine contents in the male rat. Biol Pharm Bull. 2001 Feb;24(2):163-6 https://doi.org/10.1248/bpb.24.163
  25. Zielhuis GA, Gijsen R, van der Guiden JWD. Menstrual disorders among dry-cleaning workers. Scand J Work Environ Health. 1989;15:238-44 https://doi.org/10.5271/sjweh.1856