한국축산식품학회지 (Food Science of Animal Resources)

  • 제28권5호
  • /
  • Pages.555-561
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  • 2008
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  • 2636-0772(pISSN)
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  • 2636-0780(eISSN)
한국축산식품학회 (Korean Society for Food Science of Animal Resources)
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영유아용 분말 조제분유의 미생물 품질분석과 위해세균 모니터링

Microbiological Quality and Potential Pathogen Monitoring for Powdered Infant Formulas from the Local Market

  • 황지연 (경원대학교 식품생물공학과) ;
  • 이지연 (국립수의과학검역원) ;
  • 박종현 (경원대학교 식품생물공학과)
  • Hwang, Ji-Yeon (Department of Food Science and Biotechnology, Kyungwon University) ;
  • Lee, Ji-Youn (National Veterinary Research and Quarantine Service) ;
  • Park, Jong-Hyun (Department of Food Science and Biotechnology, Kyungwon University)
  • 발행 : 2008.12.31
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초록

국내에서 생산, 유통되고 있는 영유아용 분말 조제분유 99제품을 수집하여 일반세균, 대장균군, FAO/WHO Category A, B, C 위해세균 분류에 따른 오염현황을 분석하였다. 일반세균수는 총 99개의 제품 중 92개의 제품에서 검출(93%)되었으며 $1.83{\pm}0.68\;Log\;CFU/g$의 분포를 보였다. 최고 $4.5\;Log\;CFU/g$가 검출된 한 제품을 제외하고는 조제분유의 총균수의 기준규격에 적합하였다. 대장균군은 조제분유 대장균군의 기준규격이 음성인 것에 비해 99개의 제품 중 12개의 제품에서 검출(12%)되었으며 $1.26{\pm}1.03\;\log\;MPN/g$의 분포를 보였다. 대장균은 한 제품에서 0.48 log MPN/g으로 검출되었다. Category A 세균인 Salmonella와 En. sakazakii의 오염도 분석 결과 총 99개의 모든 제품에서 검출되지 않았다. Category B group인 세균 중에서 장내세균은 99개의 제품 중 25개의 제품에서 검출(25%)되었고 오염수준은 $0.83{\pm}1.37\;\log\;MPN/g$으로 나타났다. 검출된 Category B group 세균들로는 Escherichia vulneris, Es. hermannii, Pantoea spp., Citrobacter koseri, Klebsiella pneumoniae, Enterobacter cloaceae 등이었다. Category C세균의 오염은 Bacillus cereus가 조제분유 99개의 제품 중에서 29개의 제품이 검출(29%)되어 비교적 높은 오염도를 보였지만 오염수준은 $0.69{\pm}0.32\;\log\;MPN/g$으로서 B. cereus의 국내 기준규격에 모두 적합하였다. 그 외의 Category C 세균은 검출되지 않았다. 국내 유통 조제분유의 주요 위해세균의 대한 미생물 오염정도가 낮아 미생물 기준 규격에 적합한 것으로 평가되었다. 그러나 Category B의 장내세균은 높은 검출율을 보였고 질환을 유발시킬 수 있는 균들이 확인되어 잠재적인 위험이 있다고 사료된다. 따라서 비살균식품인 조제분유의 특성상 잠재적인 위해세균 모니터링이 지속적으로 필요하며 그에 대한 안전성 확보에 능동적 자세가 필요하다.

Ninety-nine samples of powdered infant formula in a market were collected from the local market and their contaminations for total aerobic bacteria, coliform, FAO/WHO Category A, B, and C pathogens were analyzed. Total aerobic bacteria were detected in 92 of 99 samples (93%) at levels of $1.83{\pm}0.68\;Log\;MPN/g$. These levels were below legal levels specified for infant formulas except for one sample detected by 4.5 Log CFU/g. Coliform was detected in 12 of 99 samples (12%) at levels of $1.26{\pm}1.03\;Log\;MPN/g$ whereas non-detection was required according to the specification of coliform in infant formulas. Escherichia coli was detected in 1 of 99 samples by 0.48 Log MPN/g. Salmonella and Enterobacter sakazakii among Category A weren't detected in all the samples. Enterobacteriaceae, Category B group, were detected in 25 samples of total 99 samples (25%) by $0.83{\pm}1.37\;Log\;MPN/g$. Enterobacteriaceae identified by API 20E were Escherichia vulneris, Es. hermannii, Pantoea spp., Citrobacter koseri, Klebsiella pneumoniae, En. cloaceae. Bacillus cereus among Category C was highly detected in 29 of 99 samples (29%) at levels of $0.69{\pm}0.32\;Log\;MPN/g$ with the most probable number count method, which were below legal levels for the specification of B. cereus in infant formulas. Clostridium perfringens, E. coli O157, Staphyloccus aureus, Listeria monocytogenes, Yersinia enterocolitica, and Campylobacter jejuni/coli were not detected. Contamination level of major pathogens was low and falls within the range of specification of infant formulas. However, Enterobacteriaceae and B.cereus showed the high prevalence and some Enterobacteriaceae causing disease were detected. Therefore, it is necessary to monitor the potential pathogens continually and reduce them to improve the microbial quality of non-sterilized powdered infant formulas.

키워드

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피인용 문헌

  1. Biofilm Formation and Low pH Viability of Cronobacter spp. (Enterobacter sakazakii) Isolated from Powdered Infant Formula and Infant Foods in Korea vol.29, pp.6, 2009, https://doi.org/10.5851/kosfa.2009.29.6.702
  2. Analysis of Microbial Contamination of Sprouts and Fresh-cut Salads in a Market vol.43, pp.4, 2011, https://doi.org/10.9721/KJFST.2011.43.4.490
  3. Distribution of six exotoxin genes and production of L2-HBL and nheA proteins in six Bacillus cereus isolates from infant formula and produce vol.24, pp.1, 2015, https://doi.org/10.1007/s10068-015-0050-y
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  5. Characteristics of enterotoxin distribution, hemolysis, lecithinase, and starch hydrolysis of Bacillus cereus isolated from infant formulas and ready-to-eat foods vol.98, pp.3, 2015, https://doi.org/10.3168/jds.2014-9042
  6. 식중독 세균과 주요 축산식품 및 가공품 조합에 대한 위해순위 결정 vol.30, pp.1, 2015, https://doi.org/10.13103/jfhs.2015.30.1.1