Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

IL-6 gene promoter polymorph isms in Korean generalized aggressive periodontitis

한국인 전반적 급진성 치주염 환자에서 IL-6 유전자 다변성에 관한 연구

  • Bang, Sun-Jung (Department of Periodontology, School of Dentistry, and Dental Science Research Institute, Chonnam National University) ;
  • Kim, Il-Shin (Department of Periodontology, School of Dentistry, and Dental Science Research Institute, Chonnam National University) ;
  • Kim, Ok-Su (Department of Periodontology, School of Dentistry, and Dental Science Research Institute, Chonnam National University) ;
  • Kim, Young-Jun (Department of Periodontology, School of Dentistry, and Dental Science Research Institute, Chonnam National University) ;
  • Chung, Hyun-Ju (Department of Periodontology, School of Dentistry, and Dental Science Research Institute, Chonnam National University)
  • 방선정 (전남대학교 치의학전문대학원 치주과학교실 및 치의학연구소) ;
  • 김일신 (전남대학교 치의학전문대학원 치주과학교실 및 치의학연구소) ;
  • 김옥수 (전남대학교 치의학전문대학원 치주과학교실 및 치의학연구소) ;
  • 김영준 (전남대학교 치의학전문대학원 치주과학교실 및 치의학연구소) ;
  • 정현주 (전남대학교 치의학전문대학원 치주과학교실 및 치의학연구소)
  • Published : 2008.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: The purpose of this study was to investigate the association of generalized aggressive periodontitis with IL-6 promoter gene single nucleotide polymorphisms(SNP). Material and Methods: The study population consisted of 52 generalized aggressive periodontitis patients(GAP) and 30 periodontally healthy control subjects, who were systemically healthy non-smokers. Genomic DNA was obtained from buccal swab. The IL-6 promotor SNP at the positions of -597, -572, and -174 were genotyped by amplifying the polymorphic region using polymerase chain reaction(PCR), restriction enzyme digestion and gel electrophoresis. Result: The genotype distributions for G/G, G/A and A/A genotypes of IL-6 -597 were 30.8%, 40.4%, and 28.8% in the GAP group and 53.3%, 40%, and 6.7% in the control group and were statistically different between 2 groups(p<0.05). Allele 2 frequency of IL-6 -597 were significantly higher in the GAP group than the control group(p<0.01). At the position of IL-6 -572, the distribution for C/C, C/G and G/G genotypes were 23.1%, 55.8% and 21.2% in the GAP group and 20%, 33.3%, and 46.7% in the control group. In female subjects, the genotype distribution were significantly different between 2 groups(p<0.01). In male subjects, allele 2 frequency of IL-6-572 was significantly lower in the GAP group than the control group(p<0.05). The genotype distribution of IL-6 -174 in the GAP group were 96.2%, 3.8% for G/G, G/C genotypes whereas only the G/G genotype was detected in the control group. Conclusion: In conclusion, significant associations were found in IL-6 gene promoter(-597, -572) polymorphisms and generalized aggressive periodontitis. Further cohort study will be necessary in larger population.

Keywords

References

  1. Korean Dental Association. 1995's report, cited from Moon HS. Suggestions for professionalization and improvement of oral health ministration in Korea. J Korean Dent Assoc 1997;6:351
  2. Armitage GC. Development of a classification system for periodontal diseases and conditions. Ann Periodontol 1999;4: 1-6 https://doi.org/10.1902/annals.1999.4.1.1
  3. Meisel P, Siegemund A, Dombrowa S et al. Smoking and polymorphism of the IL-1 gene cluster(IL-1$\alpha$, IL-1$\beta$, and IL-1RN) in patients with periodontal disease. J Periodontol 2002;73:27-32 https://doi.org/10.1902/jop.2002.73.1.27
  4. Kornman KS, Crane A, Wang H-Y, et al. The interleukin-1 genotype as a severity factor in adult periodontal disease. J Clin Periodontol 1997;24:72-77 https://doi.org/10.1111/j.1600-051X.1997.tb01187.x
  5. Nam SJ, Chung HJ, Kim OS, Kim YJ, Koh JT. IL-1 gene polymorphisms in Korean periodontitis patients. J Kor Acad Periodontol 2004;34:623-638 https://doi.org/10.5051/jkape.2004.34.3.623
  6. Shin SY, Kim KH, Park OJ, et al. Genetic polymorphisms of the periodontally healthy Korean population. J Kor Acad Periodontol 2003;33:739-745 https://doi.org/10.5051/jkape.2003.33.4.739
  7. Yavuzyilmaz E, Yamalik N, Bulut S, et al. The gingival crevicular fluid interleukin-1$\beta$ and tumor necrosis factor-$\alpha$ levels in patients with rapidly progressive periodontitis. Aust Dent J 1995;40:46-49 https://doi.org/10.1111/j.1834-7819.1995.tb05614.x
  8. Loos BG, Craandijk J, Hoek FJ, Wertheim-van Dillen PME, van der Velden U. Elevation of systemic markers related to cardiovascular diseases in the peripheral blood of periodontitis patients. J Periodontol 2000;71:1528-1534 https://doi.org/10.1902/jop.2000.71.10.1528
  9. Kishimoto T. The biology of IL-6. Blood 1989;74:1-10
  10. Terry CF, Loukaci V, Green FR. Cooperative influence of genetic polymorphisms on interleukin 6 transcriptional regulation. J Biol Chem 2000;275:18138-18144 https://doi.org/10.1074/jbc.M000379200
  11. Gemmell E, Marshall RI, Seymour GJ. Cytokines and prostaglandins in immune homeostasis and tissue destruction in periodontal disease. Periodontol 2000 1997;14:112-143 https://doi.org/10.1111/j.1600-0757.1997.tb00194.x
  12. Hughes FJ, Howells GL. Interleukin-6 inhibits bone formation in vitro. Bone Miner 1993;21:21-28 https://doi.org/10.1016/S0169-6009(08)80117-1
  13. Irwin CR, Myrillas TT. The role of IL-6 in the pathogenesis of periodontal disease. Oral Dis 1998;4:43-47 https://doi.org/10.1111/j.1601-0825.1998.tb00255.x
  14. Takasaki K, Takashiba S, Nagai A. Assessment of IL-6 in the pathogenesis of periodontal disease. J Periodontol 1994;65:147-153 https://doi.org/10.1902/jop.1994.65.2.147
  15. Fishman D, Faulds G, Jeffery R. The effect of novel polymorphisms in the interleukin-6 (IL-6) gene on IL-6 transcription and plasma IL-6 levels, and an association with systemic-onset juvenile chronic arthritis. J Clin Invest 1998;102:1369-1376 https://doi.org/10.1172/JCI2629
  16. Trevilatto PC, Scarel-Caminaga RM, de Brito RB, de Souza AP, Line SRP. Polymorphism at position -174 of IL-6 gene is associated with susceptibility to chronic periodontitis in chronic periodontitis in a Caucasian Brazilian population. J Clin Periodontol 2003;30:438-442 https://doi.org/10.1034/j.1600-051X.2003.20016.x
  17. Holla LI, Fassmann A, Stejskalova A, et al. Analysis of the interleukin-6 gene promoter polymorphisms in Czech patients with chronic periodontitis. J Periodontol 2004;75: 30-36 https://doi.org/10.1902/jop.2004.75.1.30
  18. Shin KK, Jang Y, Koh SJ, et al. Influence of the IL-6 -572C>G polymorphism on inflammatory markers according to cigarette smoking in Korean healthy men. Cytokine 2007;39:116-122 https://doi.org/10.1016/j.cyto.2007.06.005
  19. Lim SC, Zheng S, Kim YS, et al. The -174 G to C polymorphism of interleukin-6 gene is very rare in Koreans. Cytokine 2002;19:52-54 https://doi.org/10.1006/cyto.2002.1951
  20. Pyo CW, Hur SS, Kim YK, et al. Polymorphisms of IL-1B, IL-1RN, IL-2, IL-4, IL-6, IL-10, and IFN-$\gamma$ Genes in the Korean Population. Human Immunol 2003;64:979-989 https://doi.org/10.1016/S0198-8859(03)00173-3
  21. Komatsu Y, Tai H, Galicia J.C, et al. Interleukin-6(IL-6) -373 A9T11 allele is associated with reduced susceptibility to chronic periodontitis in Japanese subjects and decreased serum level IL-6 level. Tissue Antigen 2005;65:110-114 https://doi.org/10.1111/j.1399-0039.2005.00347.x
  22. Masada M. P, Persson R, Kenney JS, et al. Measurment of interleukin-1$\alpha$ and -1$\beta$ in gingival crevicular fluid: implications for the pathogenesis of periodontal disease. J Periodont Res 1990;25:156-163 https://doi.org/10.1111/j.1600-0765.1990.tb01038.x
  23. Takashiba S, Naruishi K. Gene polymorphisms in periodontal health and disease. Periodontol 2000 2006;40: 94-106 https://doi.org/10.1111/j.1600-0757.2005.00142.x
  24. Lotz M, Jirik F, Kabouridis P, et al. B cell stimulating factor 2/ IL-6 is a costimulant for human thymocytes and T lymphocytes. J Exp Med 1988;167:1253-1258 https://doi.org/10.1084/jem.167.3.1253
  25. Nibali L, Griffiths GS, Donos N, et al. Association between interleukin-6 promotor haplotypes and aggressive periodontitis. J Clin Periodontol 2008;35:193-198 https://doi.org/10.1111/j.1600-051X.2007.01188.x
  26. Nibali L, Ready DR, Parker M, et al. Gene polymorphisms and the prevalence of key periodontal pathogens. J Dent Res 2007;86:416-420 https://doi.org/10.1177/154405910708600505
  27. D'Auito F, Ready D, Parker M, Tonetti MS. Relative contribution of patient-, tooth-, and site-associated variability on the clinical outcomes of subgingival debridement. I. Probing depth. J Periodontol;76:398-405 https://doi.org/10.1902/jop.2005.76.3.398
  28. Brull DJ, Montgomery HE, Sanders J, et al. Interleukin-6 gene -174G>C and -572G>C promoter polymorphisms are strong predictors of plasma interleukin-6 levels after coronary artery bypass surgery. Arterioscler Thromb Vasc Biol 2001;21:1458-1463 https://doi.org/10.1161/hq0901.094280
  29. Chang HK, Jang WC, Park SB, et al. Association between interleukin 6 gene polymorphisms and Behcet's disease in Korean people. Ann Rheum Dis 2005;64:339-40 https://doi.org/10.1136/ard.2004.024208
  30. Brett PM, Zygogianni P, Griffiths GS, et al. Functional gene polymorphisms in aggressive and chronic periodontitis. J Dent Res 2005;84:1149-1153 https://doi.org/10.1177/154405910508401211