Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Ameliorating Effects of the Ethanol Extracts from Gynostemma pentaphyllum on Electric Footshock Stress

돌외 에탄올 추출물 엑스의 전기쇼크 스트레스 저항력 개선작용

  • Published : 2008.12.31
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Abstract

The effects of the ethanol extracts from Gynostemma pentaphyllum (GP extracts) on body weights, grip strengths, endurances and catecholamine levels after electric footshock (EF) stress in mice and rats were investigated. The animals were treated with GP extracts (50 mg/kg/day, p.o.) for 21 days before exposure to EF (duration and interval 10 sec for 3 min, 2 mA) once a day. The increases in body weights were delayed by 13.1% of the control levels by EF-induced stress in mice, which were recovered to 24.1% of the control levels in GP extract-treated groups. The grip strengths were significantly decreased by EF stress in mice and the EF-stressed groups treated with GP extracts increased grip strengths to 115.2% compared to control levels. The endurance times by forced swimming, which reduced significantly by EF stress, were also maintained similar to control levels by GP extracts in rats. In addition, the levels of norepinephrine and epinephrine in serum and brain, and dopamine in brain were significantly increased to 17.5-95.0% of the control levels after exposure of EF stress in mice. However, EF stressinduced increases in norepinephrine and epinephrine in serum were reduced to 17.1-17.3% of the control levels by treatments of GP extracts, and those in dopamine, norepinephrine and epinephrine in brain were also reduced to 5.0-19.5%. These results suggest that GP extracts showed the protective effects on EF stress-induced physiological functions and can be developed as the promising anti-stress agents.

Keywords

References

  1. Yoshikawa, K., Mitake, M., Takemoto, T. and Arihara, S. (1987) Studies on the constituents of Curcubitaceae plants. XVII. On the saponins constituents of Gynostemma pentaphyllum Makino (12). Yakugaku. Zasshi 107: 355-360 https://doi.org/10.1248/yakushi1947.107.5_355
  2. Kuwahara, M., Kawanishi, F., Komiya, T. and Oshio, H. (1989) Dammarane saponins from Gynostemma pentaphylllum Makino and isolation of malonylginsenosides-$Rb_{1}$, -Rd, and malonylgypenoside V. Chem. Pharm. Bull. 37: 135-139 https://doi.org/10.1248/cpb.37.135
  3. Blumert, M. L. and Liu, J. (2003) Jiaogulan, China immortality herb, 12-20, Torchlight Publishing, Inc., Badger, CA, USA
  4. Wang, Z. J. and Luo, D. H. (2007) Antioxidant activities of different fractions of polysaccharide purified from Gynostemma pentaphyllum Makino. Carbohyd. Polym. 68: 54-58 https://doi.org/10.1016/j.carbpol.2006.07.022
  5. Huang, T. H., Tran, V. H., Roufogalis, B. D. and Li, Y. (2007) Gypenoside XLIX, a naturally occurring PPAR-alpha activator, inhibits cytokine-induced vascular cell adhesion molecule-1 expression and activity in human endothelial cells. Eur. J. Pharmacol. 565: 158-165 https://doi.org/10.1016/j.ejphar.2007.03.013
  6. Circosta, C., De Pasquale, R. and Occhiuto, F. (2005) Car-diovascular effects of the aqueous extract of Gynostemma pentaphyllum Makino. Phytomedicine 12: 638-643 https://doi.org/10.1016/j.phymed.2004.06.023
  7. Megallia, S., Aktanb, Fugen., Daviesc, N. M. and Roufogalisa, B. D. (2005) Phytopreventative anti-hyperlipidemic effects of Gynostemma pentaphyllum in rats. J. Pharm. Pharmaceut. Sci. 8: 507-515
  8. Huang, W. C., Kuo, M. L., Li, M. L., Yang, R. C., Liou, C. J. and Shen, J. J. (2007) The extract of Gynostemma pentaphyllum enhanced the production of antibodies and cytokines in mice. Yankugaku Zasshi 127: 889-896 https://doi.org/10.1248/yakushi.127.889
  9. Hou, J., Liu, S., MA, Z., Lang, X., Wang, J., Wang, J.and Liang, Z. (1991) Effects of Gynostemma pentaphyllum Makino on the immunological functions of cancer patients. J. Trad. Chin. Med. 11: 47-52
  10. Norberg, A., Hoa, N. K., Liepinsh, E., Van Phan D., Thuan, N. D., Jornvall, H., Sillard, R. and Ostenson, C. G. (2004) A novel insulin-releasing substance, phanoside, from the plant Gynostemma pentaphyllum. J. Biol. Chem. 279: 41361-41367 https://doi.org/10.1074/jbc.M403435200
  11. Lin, J. M., Lin, C. C., Chiu, H. F., Yang, J. J. and Lee, S. G. (1993) Evaluation of the anti-inflammatory and liver-protective effects of Anoectochilus formosanus, Ganoderma lucidum and Gynostemma pentaphyllum in rats. Am. J. Chin. Med. 21: 59-69 https://doi.org/10.1142/S0192415X9300008X
  12. Rujjanawate, C., Kanjanapothi, D. and Amornlerdpison, D. (2004) The anti-gastric ulcer effect of Gynostemma pentaphyllum Makino. Phytomedicine 11: 431-435 https://doi.org/10.1016/j.phymed.2003.07.001
  13. Yan Y-Q (chief ed.), (1996) Encyclopedia of Chinese Herbs, Vol 2, 1878-1882, China Pharmaceutical University, China Medicine, Science and Technology Publisher, China
  14. Ulrich-Lai, P. M and Engeland, W. C. (2005) Sympatho-adrenal activity and hypothalamic-pituitary-adrenal axis regulation. In Steckler, T., Kalin, N. H., Reul, J. M. H. M. (ed.). Handbook of stress and the brain, Part I, 419-436, Elsevier
  15. Kovacs, K. J., Mikios, I. H. and Bali, B. (2005) Psychological and physical stressors. In Steckler, T., Kalin, N. H., Reul, J. M. H. M. (ed.). Handbook of stress and the brain, Part I, 775-792, Elsevier
  16. Sorg, B. A. and Kalivas, P. W. (1991) Effects of cacaine and footshock stress on extracellular dopamine levles in the ventral striatum. Brain Res. 559: 29-36 https://doi.org/10.1016/0006-8993(91)90283-2
  17. Saurez-Roca, H., Quintero, L., Arcaya, J. L., Maixner, W. and Rao, S. D. (2006) Stress-induced and cutaneous hyperalgesia: Differential effect of milnacipran. Physiol. Beh. (2006) 88: 82-87 https://doi.org/10.1016/j.physbeh.2006.03.010
  18. Zhou, S. R. and Qiu, Z. R. (1990) A preliminary study on the effects of Gynostemma pentaphyllum on endurance, spontaneous motor activity and superoxide dismutase in mice. Asia. Pacific J. Pharmacol. 5: 321-322
  19. Mitsui, A., Nohta, H. and Ohkura, Y. (1985) High-performance liquid chromatography of plasma catecholamines using 1,2-diphenylethylenediamine as precolumn fluorescence derivatization reagent. J. Chromatogr. 344: 61-70 https://doi.org/10.1016/S0378-4347(00)82007-1
  20. Attawish, A., Chivapat, S., Phadungpat, S., Bansiddhi, J., Techadamrongsin, Y., Mitrijit, O., Chaorai, B. and Chavalittumrong, P. (2004) Chronic toxicity of Gynostemma pentaphyllum. Fitoterapia 75: 539-551 https://doi.org/10.1016/j.fitote.2004.04.010
  21. Tsuda, A., Tanaka, M., Ida, Y., Tsujimaru, S., Ushijima, I., Nagasaki, N. (1986) Effects of preshock experience on enhancement of rat grain noradrenaline turnover induced by psychological stress. Pharmacol. Biochem. Behav. 24: 115-119 https://doi.org/10.1016/0091-3057(86)90053-5
  22. 중소기업기술혁신개발사업 최종보고서, 중소기업청. (2007. 9. 28.), '생약 조성물을 이용한 항스트레스제 기능성 식품 개발', (주)브레인트로피아, 충북대학교 생물건강산업개발 연구센터
  23. 신진이, 이상철, 최수진, 조한, 염제호, 유은주, 김영만. (1998. 8) 항스트레스 조성물(특허등록번호 제10-016040)
  24. 김미리, 석대은, 오상희, 김윤배. (2005. 12) 신경세포 보호효과 및 항산화 활성을 갖는 머위추출물(특허등록번호 제10-0535655)
  25. 신정수, 이상선, 김응일, 심성민, 이명구. (1997) Berberine이 구속 스트레스 부가후 mouse의 혈중 catecholamine 함량에 미치는 영향. Kor. J. Clin. Pharm. 7: 81-85