미생물학회지 (Korean Journal of Microbiology)

  • 제44권2호
  • /
  • Pages.98-104
  • /
  • 2008
  • /
  • 0440-2413(pISSN)
  • /
  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

분열효모에서 mRNA Export와 관련된 rgm1 유전자의 유전학적 분석

Genetic Analysis of Fission Yeast rsm1 Which is Involved in mRNA Export

  • 강숙희 (성신여자대학교 자연과학대학 생물학과 및 기초과학연구소) ;
  • 윤진호 (성신여자대학교 자연과학대학 생물학과 및 기초과학연구소)
  • Kang, Su-Ky (Basic Science Research Institute and Department of Biology, College of Natural Sciences, Sungshin Women's University) ;
  • Yoon, Jin-Ho (Basic Science Research Institute and Department of Biology, College of Natural Sciences, Sungshin Women's University)
  • 발행 : 2008.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

mRNA의 핵에서 세포질로의 이동(mRNA export)에 관여하는 것으로 여겨지는 분열효모 Schizosaccharomyces pombe의 rsm1 유전자의 역할을 알아보기 위해 $kan^{r}$ 유전자를 이용하여 결실돌연변이주(deletion mutant)를 제조하였다. rsm1 유전자는 생장에 필수 유전자는 아니지만, rsm1 결실돌연변이주는 야생형에 비해 생장이 조금 늦고 mRNA export도 약간의 결함을 보였다. rsm1 유전자와 mRNA export의 중요 유전자와의 연관관계를 알아보기 위해, 이중돌연변이주(double mutants)를 제작하여 생장결함 정도와 mRNA export 결함 정도를 조사하였다. 조사한 유전자들 중에서 mex67 또는 npp106 돌연변이 유전자는 rsm1 결실돌연변이 유전자와 함께 존재하면 생장과mRNA export가 더욱 악화되었다. 반면, thp1 돌연변이 유전자는 rsm1 결실돌연변이 유전자와 함께 존재하면 오히려 생장과 mRNA export 정도를 야생형과 유사한 정도로 호전시켰다. 이와 같은 결과들은 rsm1 유전자가 mRNA의 핵에서 세포질로의 이동에 중요한 역할을 담당하고 있음을 시사한다.

We constructed the null mutants of fission yeast Schizosaccharomyces pombe rsml gene that is thought to be involved in mRNA export. Though rsm1 gene is not essential for growth, the null mutant strain constructed by replacing the rsm1-coding region with an $kan^{r}$ gene showed growth retardation and mRNA export defects compared to wild type strain. We constructed double mutants which harbor rsm1 null allele and mutant allele of genes involved in mRNA export. The mex67 or npp106 null allele, when combined with rsm1 null allele, showed an additive effect on growth retardation and mRNA export defects. On the other hand, the thp1 null allele restored the defects of growth and mRNA export of rsm1 null mutant. These results suggest that rsm1 plays a role in mRNA export from the nucleus.

키워드

참고문헌

  1. Alfa, C., P. Fantes, J. Hyams, M. Mcleod, and E. Warbrick. 1993. Experiments with fission yeast. Cold Spring Harbor, Cold Spring Harbor Laboratory, New York, NY, USA
  2. Cronshaw, J.M., A.N. Krutchinsky, W. Zhang, B.T. Chait, and M.J. Matunis. 2002. Proteomic analysis of the mammalian nuclear pore complex. J. Cell Biol. 158, 915-927 https://doi.org/10.1083/jcb.200206106
  3. Cullen, B.R. 2003. Nuclear RNA export. J. Cell Sci. 116, 587-597 https://doi.org/10.1242/jcs.00268
  4. Darzacq, X., R.H. Singer, and Y. Shav-Tal. 2005. Dynamics of transcription and mRNA export. Curr. Opin. Cell Biol. 17, 332-339 https://doi.org/10.1016/j.ceb.2005.04.004
  5. Dimaano, C. and K.S. Ullman. 2004. Nucleocytoplasmic transport: integrating mRNA production and turnover with export through the nuclear pore. Mol. Cell Biol. 24, 3069-3076 https://doi.org/10.1128/MCB.24.8.3069-3076.2004
  6. Erkmann, J.A. and U. Kutay. 2004. Nuclear export of mRNA: from the site of transcription to the cytoplasm. Exp. Cell Res. 296, 12-20 https://doi.org/10.1016/j.yexcr.2004.03.015
  7. Forsburg, S.L. 1993. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21, 2955-2956 https://doi.org/10.1093/nar/21.12.2955
  8. Huang, Y. and J.A. Steitz. 2005. SRprises along a messenger's journey. Mol. Cell 17, 613-615 https://doi.org/10.1016/j.molcel.2005.02.020
  9. Izaurralde, E. 2002. A novel family of nuclear transport receptors mediates the export of messenger RNA to the cytoplasm. Eur. J. Cell Biol. 81, 577-584 https://doi.org/10.1078/0171-9335-00273
  10. Khler, A. and E. Hurt. 2007. Exporting RNA from the nucleus to the cytoplasm. Nat. Rev. Mol. Cell Biol. 8, 761-773 https://doi.org/10.1038/nrm2255
  11. Maundrell, K. 1993. Thiamine-repressible expression vectors pREP and pRIP for fission yeast. Gene 123, 127-130 https://doi.org/10.1016/0378-1119(93)90551-D
  12. Moore, M.J. 2005. From birth to death: the complex lives of eukaryotic mRNAs. Science 309, 1514-1518 https://doi.org/10.1126/science.1111443
  13. Moreno, S., A. Klar, and P. Nurse. 1991. Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol. 194, 795-823 https://doi.org/10.1016/0076-6879(91)94059-L
  14. Palancade, B., M. Zuccolo, S. Loeillet, A. Nicolas, and V. Doye. 2005. Pml39, a novel protein of the nuclear periphery required for nuclear retention of improper messenger ribonucleoparticles. Mol. Biol. Cell 16, 5258-5268 https://doi.org/10.1091/mbc.E05-06-0527
  15. Reed, R. and E. Hurt. 2002. A conserved mRNA export machinery coupled to pre-mRNA splicing. Cell 108, 523-531 https://doi.org/10.1016/S0092-8674(02)00627-X
  16. Rodriguez-Navarro, S., T. Fischer, M.J. Luo, O. Antunez, S. Brettschneider, J. Lechner, J.E. Perez-Ortin, R. Reed, and E. Hurt. 2004. Sus1, a functional component of the SAGA histone acetylase complex and the nuclear pore-associated mRNA export machinery. Cell 116, 75-86 https://doi.org/10.1016/S0092-8674(03)01025-0
  17. Rout, M.P., J.D. Aitchison, A. Suprapto, K. Hjertaas, Y. Zhao, and B.T. Chait. 2000. The yeast nuclear pore complex: composition, architecture, and transport mechanism. J. Cell Biol. 148, 635-651 https://doi.org/10.1083/jcb.148.4.635
  18. Segref, A., K. Sharma, V. Doye, A. Hellwig, J. Huber, R. Luhrmann, and E. Hurt. 1997. Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores. EMBO J. 16, 3256-3271 https://doi.org/10.1093/emboj/16.11.3256
  19. Strasser, K. and E. Hurt. 2000. Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export. EMBO J. 19, 410-420 https://doi.org/10.1093/emboj/19.3.410
  20. Strasser, K. and E. Hurt. 2001. Splicing factor Sub2p is required for nuclear mRNA export through its interaction with Yra1p. Nature 413, 648-652 https://doi.org/10.1038/35098113
  21. Stutz, F. and E. Izaurralde. 2003. The interplay of nuclear mRNP assembly, mRNA surveillance and export. Trends Cell. Biol. 13, 319-327 https://doi.org/10.1016/S0962-8924(03)00106-5
  22. Suntharalingam, M. and S.R. Wente. 2003. Peering through the pore: nuclear pore complex structure, assembly, and function. Dev. Cell 4, 775-789 https://doi.org/10.1016/S1534-5807(03)00162-X
  23. Thakurta, A.G., G. Gopal, J.H. Yoon, L. Kozak, and R. Dhar. 2005. Homolog of BRCA2-interacting Dss1p and Uap56p link Mlo3p and Rae1p for mRNA export in fission yeast. EMBO J. 24, 2512-2523 https://doi.org/10.1038/sj.emboj.7600713
  24. Thakurta, A.G., G. Gopal, J.H. Yoon, T. Saha, and R. Dhar. 2004. Conserved nuclear export sequences in Schizosaccharomyces pombe Mex67 and human TAP function in mRNA export by direct nuclear pore interactions. J. Biol. Chem. 279, 17434-17442 https://doi.org/10.1074/jbc.M309731200
  25. Tran, E.J. and S.R. Wente. 2006. Dynamic nuclear pore complexes: life on the edge. Cell 125, 1041-1053 https://doi.org/10.1016/j.cell.2006.05.027
  26. Vinciguerra, P. and F. Stutz. 2004. mRNA export: an assembly line from genes to nuclear pores. Curr. Opin. Cell Biol. 16, 285-292 https://doi.org/10.1016/j.ceb.2004.03.013
  27. Whalen, W.A., J.H. Yoon, R. Shen, and R. Dhar. 1999. Regulation of mRNA export by nutritional status in fission yeast. Genetics 152, 827-838
  28. Yoon, J.H. 2003. Synthetic lethal mutations with spmex67 of Schizosaccharomyces pombe in the mediation of mRNA export. J. Microbiol. 41, 115-120
  29. Yoon, J.H. 2004. Schizosaccharomyces pombe rsm1 genetically interacts with spmex67, which is involved in mRNA export. J. Microbiol. 42, 32-36
  30. Yoon, J.H. 2006. Construction of Schizosaccharomyces pombe spThp1 null mutants and its characterization. Kor. J. Microbiol. 42, 149-152
  31. Yoon, J.H., D. Love, A. Guhathakurta, J.A. Hanover, and R. Dhar. 2000. Mex67p of Schizosaccharomyces pombe interacts with Rae1p in mediating mRNA export. Mol. Cell. Biol. 20, 8767-8782 https://doi.org/10.1128/MCB.20.23.8767-8782.2000
  32. Yoon, J.H., W.A. Whalen, A. Bharathi, R. Shen, and R. Dhar. 1997. Npp106p, a Schizosaccharomyces pombe nucleoporin similar to Saccharomyces cerevisiae Nic96p, functionally interacts with Rae1p in mRNA export. Mol. Cell. Biol. 17, 7047-7060 https://doi.org/10.1128/MCB.17.12.7047