The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Inhibitory Effect of Angelica gigas Nakai Extract on Nitric Oxide Production in RAW 264.7 Cells

대식세포에서 산화질소 생성에 대한 당귀(當歸) 에탄올 추출물의 억제효과

  • Jeong, Mi-Young (WHO Collaborating Center for Traditional Medicine, East-West Medical Research Institute, Kyunghee University) ;
  • Park, Hi-Joon (Kyunghee University, Department of Meridian and Acupuncture, College of Eastern Medicine, Kyunghee University) ;
  • Jeong, Jee-Haeng (Jeong Jeehaeng Rhythmcare Co. Ltd) ;
  • Kim, Jin-Young (WHO Collaborating Center for Traditional Medicine, East-West Medical Research Institute, Kyunghee University) ;
  • Kang, Jun-Mo (WHO Collaborating Center for Traditional Medicine, East-West Medical Research Institute, Kyunghee University, Department of Meridian and Acupuncture, College of Eastern Medicine, Kyunghee University) ;
  • Lee, Na-Kyeong (WHO Collaborating Center for Traditional Medicine, East-West Medical Research Institute, Kyunghee University, Department of Meridian and Acupuncture, College of Eastern Medicine, Kyunghee University) ;
  • Lim, Sabina (WHO Collaborating Center for Traditional Medicine, East-West Medical Research Institute, Kyunghee University, Department of Meridian and Acupuncture, College of Eastern Medicine, Kyunghee University)
  • 정미영 (경희대학교 WHO 전통의학연구협력센터 동서의학연구소) ;
  • 박히준 (경희대학교 한의과대학 경혈학교실) ;
  • 정지행 (정지행리듬케어(주)) ;
  • 김진용 (경희대학교 WHO 전통의학연구협력센터 동서의학연구소) ;
  • 강전모 (경희대학교 WHO 전통의학연구협력센터 동서의학연구소, 경희대학교 한의과대학 경혈학교실) ;
  • 이나경 (경희대학교 WHO 전통의학연구협력센터 동서의학연구소, 경희대학교 한의과대학 경혈학교실) ;
  • 임사비나 (경희대학교 WHO 전통의학연구협력센터 동서의학연구소, 경희대학교 한의과대학 경혈학교실)
  • Published : 2007.06.30
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Abstract

Objective : The Angelica gigas Nakai ethanol extract (AGE) was investigated to compare nitric oxide (NO) production and $NF-{\kappa}B$ activity from RAW 264.7 cells, since NO and nuclear $factor-{\kappa}B$ $(NF-{\kappa}B)$ have been shown to be factors implicated in inflammatory disease. Method : AGE was prepared by extracting medicinal herb with 70% (v/v) ethanol solution. We investigated production of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) gene expression by ARE in LPS-stimulated RAW 264.7 macrophage cells. We also investigated inhibition of LPS-induced activation of $NF-{\kappa}B$ on western blot. Result : LPS-induced RAW 264.7 cells increased NO production and iNOS expression. Upon treatment with AGE, nitrite production was significantly inhibited in a concentration-dependent manner compared to the untreated control. AGE inhibited this LPS-induced iNOS mRNA and protein in a dose-dependent manner. AGE markedly inhibited the expression of iNOS mRNA and protein at a concentration of 100 ${\mu}g/ml$. LPS-induced RAW 264.7 cells with AGE blocked inhibitory $factor-{\kappa}B{\alpha}$ degradation. Conclusion :This study shows that AGE seems to attenuate inflammation through inhibition of NO production and iNOS expression by blockade of $NF-{\kappa}B$ activation in LPS-stimulated RAW 264.7 cells.

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