대한한의학회지 (The Journal of Korean Medicine)

대한한의학회 (The Society of Korean Medicine)
권호 표지

대식세포에서 산화질소 생성에 대한 당귀(當歸) 에탄올 추출물의 억제효과

Inhibitory Effect of Angelica gigas Nakai Extract on Nitric Oxide Production in RAW 264.7 Cells

  • 정미영 (경희대학교 WHO 전통의학연구협력센터 동서의학연구소) ;
  • 박히준 (경희대학교 한의과대학 경혈학교실) ;
  • 정지행 (정지행리듬케어(주)) ;
  • 김진용 (경희대학교 WHO 전통의학연구협력센터 동서의학연구소) ;
  • 강전모 (경희대학교 WHO 전통의학연구협력센터 동서의학연구소, 경희대학교 한의과대학 경혈학교실) ;
  • 이나경 (경희대학교 WHO 전통의학연구협력센터 동서의학연구소, 경희대학교 한의과대학 경혈학교실) ;
  • 임사비나 (경희대학교 WHO 전통의학연구협력센터 동서의학연구소, 경희대학교 한의과대학 경혈학교실)
  • Jeong, Mi-Young (WHO Collaborating Center for Traditional Medicine, East-West Medical Research Institute, Kyunghee University) ;
  • Park, Hi-Joon (Kyunghee University, Department of Meridian and Acupuncture, College of Eastern Medicine, Kyunghee University) ;
  • Jeong, Jee-Haeng (Jeong Jeehaeng Rhythmcare Co. Ltd) ;
  • Kim, Jin-Young (WHO Collaborating Center for Traditional Medicine, East-West Medical Research Institute, Kyunghee University) ;
  • Kang, Jun-Mo (WHO Collaborating Center for Traditional Medicine, East-West Medical Research Institute, Kyunghee University, Department of Meridian and Acupuncture, College of Eastern Medicine, Kyunghee University) ;
  • Lee, Na-Kyeong (WHO Collaborating Center for Traditional Medicine, East-West Medical Research Institute, Kyunghee University, Department of Meridian and Acupuncture, College of Eastern Medicine, Kyunghee University) ;
  • Lim, Sabina (WHO Collaborating Center for Traditional Medicine, East-West Medical Research Institute, Kyunghee University, Department of Meridian and Acupuncture, College of Eastern Medicine, Kyunghee University)
  • 발행 : 2007.06.30
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초록

Objective : The Angelica gigas Nakai ethanol extract (AGE) was investigated to compare nitric oxide (NO) production and $NF-{\kappa}B$ activity from RAW 264.7 cells, since NO and nuclear $factor-{\kappa}B$ $(NF-{\kappa}B)$ have been shown to be factors implicated in inflammatory disease. Method : AGE was prepared by extracting medicinal herb with 70% (v/v) ethanol solution. We investigated production of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) gene expression by ARE in LPS-stimulated RAW 264.7 macrophage cells. We also investigated inhibition of LPS-induced activation of $NF-{\kappa}B$ on western blot. Result : LPS-induced RAW 264.7 cells increased NO production and iNOS expression. Upon treatment with AGE, nitrite production was significantly inhibited in a concentration-dependent manner compared to the untreated control. AGE inhibited this LPS-induced iNOS mRNA and protein in a dose-dependent manner. AGE markedly inhibited the expression of iNOS mRNA and protein at a concentration of 100 ${\mu}g/ml$. LPS-induced RAW 264.7 cells with AGE blocked inhibitory $factor-{\kappa}B{\alpha}$ degradation. Conclusion :This study shows that AGE seems to attenuate inflammation through inhibition of NO production and iNOS expression by blockade of $NF-{\kappa}B$ activation in LPS-stimulated RAW 264.7 cells.

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