참다래의 친환경재배를 위한 과숙썩음병원균에 대한 길항성 방선균 #120의 선발 및 분리

Screening and Isolation of Antagonistic Actinomyces #120 against the Kiwi Fruit Rot for the Environment-Friendly Culture of Kiwifruits

  • 조정일 (조선이공대학 식품공학과) ;
  • 조자용 (남도대학 약용자원원예개발과) ;
  • 박용서 (목포대학교 응용생명과학부) ;
  • 손동모 (순천대학교 식물생산과학부) ;
  • 허북구 ((재)나주시천연염색문화재단) ;
  • 김철수 (원광대학교 원예.애완동식물학부)
  • Cho, Jung-Il (Dept. of Food Technology, Chosun College of Science and Technology) ;
  • Cho, Ja-Yong (Dept. of Medicinal Resources & Horticulture Development, Jeonnam Provincial College) ;
  • Park, Yong-Seo (Division of Applied Bioscience, Mokpo National Univ.) ;
  • Son, Dong-Mo (Jeonnam Agricultural Research & Extension Services) ;
  • Heo, Buk-Gu (Naju Foundation of Natural Dyeing Culture) ;
  • Kim, Chul-Soo (Division of Horticulture and Pet Animal-Plant Science, Wonkwang University)
  • 발행 : 2007.09.30

초록

전남 서남부지역의 참다래 과수원에서 발생하는 과숙썩음병원균(fruit rot, Botryosphaeria dothidea)에 대한 항균작용이 우수한 세균성 균주를 선발하기 위하여 참다래 과수원 토양으로부터 단일균주를 분리하였으며, 과숙썩음병원균(Botryosphaeria dothidea)에 대한 생물적 제어 능력을 검정하고 균주 동정을 실시하였다. 참다래 과수원에서 분리한 총 250여종의 단일균주 중에서 참다래에서 발생하는 과숙척음병원균에 대하여 길항작용이 우수한 균주를 1차적으로 6종 선발하였고, 이 중에서 참다래 과숙썩음병원균에 대하여 길항 작용이 96.0% 정도로 우수한 Strain #120을 최종적으로 선발하였다. 길항균 #120의 포자배열은 rectiflexibles 하였고, 포자표면은 smooth형이었으며, 분리균 세포벽내 LL-type이 DAP를 갖는 wall chemotype I 이었다. 길항균 #120의 균주 형태학적 특성, 생리 생화학적 그리고 화학 분류학적 특성 등을 종합하여 볼 때 길항균 #120은 Streptomyces sp. #120으로 동정되었다.

This study was carried out to clarify the effects of antifungal Streptomyces sp. isolated from the soil grown kiwifruit on the growth inhibition of fruit rot (Botryosphaeria dothidea) infected in kiwi fruit plants in the southwestern districts of Jeonnam. Two hundred and fifty microorganisms were isolated and examined into the antifungal activity against Botryosphaeria dothidea. We screened and isolated six bacterial strains which have a strong inhibition against Botryosphaeria dothidea. And the best antifungal strain designated as the strain #120 showing 96.0% antifungal activity against Botryosphaeria dothidea was finally selected. The strain #120 was identified as Streptomyces sp. #120 based on its morphological, physiological, biochemical and chemotaxonomic characteristics.

키워드

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