Journal of Bio-Environment Control (생물환경조절학회지)

The Korean Society for Bio-Environment Control (한국생물환경조절학회)
권호 표지

Screening and Isolation of Antagonistic Actinomyces #120 against the Kiwi Fruit Rot for the Environment-Friendly Culture of Kiwifruits

참다래의 친환경재배를 위한 과숙썩음병원균에 대한 길항성 방선균 #120의 선발 및 분리

  • Cho, Jung-Il (Dept. of Food Technology, Chosun College of Science and Technology) ;
  • Cho, Ja-Yong (Dept. of Medicinal Resources & Horticulture Development, Jeonnam Provincial College) ;
  • Park, Yong-Seo (Division of Applied Bioscience, Mokpo National Univ.) ;
  • Son, Dong-Mo (Jeonnam Agricultural Research & Extension Services) ;
  • Heo, Buk-Gu (Naju Foundation of Natural Dyeing Culture) ;
  • Kim, Chul-Soo (Division of Horticulture and Pet Animal-Plant Science, Wonkwang University)
  • 조정일 (조선이공대학 식품공학과) ;
  • 조자용 (남도대학 약용자원원예개발과) ;
  • 박용서 (목포대학교 응용생명과학부) ;
  • 손동모 (순천대학교 식물생산과학부) ;
  • 허북구 ((재)나주시천연염색문화재단) ;
  • 김철수 (원광대학교 원예.애완동식물학부)
  • Published : 2007.09.30
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Abstract

This study was carried out to clarify the effects of antifungal Streptomyces sp. isolated from the soil grown kiwifruit on the growth inhibition of fruit rot (Botryosphaeria dothidea) infected in kiwi fruit plants in the southwestern districts of Jeonnam. Two hundred and fifty microorganisms were isolated and examined into the antifungal activity against Botryosphaeria dothidea. We screened and isolated six bacterial strains which have a strong inhibition against Botryosphaeria dothidea. And the best antifungal strain designated as the strain #120 showing 96.0% antifungal activity against Botryosphaeria dothidea was finally selected. The strain #120 was identified as Streptomyces sp. #120 based on its morphological, physiological, biochemical and chemotaxonomic characteristics.

전남 서남부지역의 참다래 과수원에서 발생하는 과숙썩음병원균(fruit rot, Botryosphaeria dothidea)에 대한 항균작용이 우수한 세균성 균주를 선발하기 위하여 참다래 과수원 토양으로부터 단일균주를 분리하였으며, 과숙썩음병원균(Botryosphaeria dothidea)에 대한 생물적 제어 능력을 검정하고 균주 동정을 실시하였다. 참다래 과수원에서 분리한 총 250여종의 단일균주 중에서 참다래에서 발생하는 과숙척음병원균에 대하여 길항작용이 우수한 균주를 1차적으로 6종 선발하였고, 이 중에서 참다래 과숙썩음병원균에 대하여 길항 작용이 96.0% 정도로 우수한 Strain #120을 최종적으로 선발하였다. 길항균 #120의 포자배열은 rectiflexibles 하였고, 포자표면은 smooth형이었으며, 분리균 세포벽내 LL-type이 DAP를 갖는 wall chemotype I 이었다. 길항균 #120의 균주 형태학적 특성, 생리 생화학적 그리고 화학 분류학적 특성 등을 종합하여 볼 때 길항균 #120은 Streptomyces sp. #120으로 동정되었다.

Keywords

References

  1. Agrios, G.N. 1997. Plant Pathology. Academic Press, San Diego
  2. Bangera, M.G and L.S. Thomashow. 1999. Identification and characterization of agene cluster for synthesis of the polyketide antibiotic 2, diacetylphloroglucinol from Pseudomonas fluorescens Q2-87. Environ Microbiol. 181:3155-3163
  3. Bloemberg, G.V. and B.J.J. Lugtenberg. 2001. Molecular basis of plant growth promotion and biocontrol by rhizobacteria. Current Opinion in Plant Biology. 4: 343-350 https://doi.org/10.1016/S1369-5266(00)00183-7
  4. Booth, C. 1971. Methods in microbiology. Academic Press, London
  5. Delany, I., M.M. Sheehan, A. Fenton, S. Bardin, S. Aarons, and F.O. Gara. 2000. Regulation of production of the antifungal metabolite 2,4-diacetylphloroglucinol in Pseudomonas fluorescens F113. Microbiology. 146:537-546 https://doi.org/10.1099/00221287-146-2-537
  6. Goodfellow, M. and E.M. David. 1985. Chemical methods in bacterial systematics. Academic Press, London & New York
  7. Gottlieb, D. and T.G. Pridham. 1948. The utilization of carbon compounds by some Actinomycetec as an aid for species determination. J. Bacteriol. 56:101
  8. Harrigan, W.F. and M.E. McCance. 1966. Laboratory method in microbiology. Academic Press, New York & London
  9. Kaneko, T., Y. Nakamura, S. Sato, E. Asamizu, T. Kato, S. Sasamoto, A. Watanabe, K. Idesawa, A. Ishikaw, and K. Kawashima. 2000. Complete genome structure of the nitrogen fixing symbiotic bacterium Mesorhizobium loti. DNA Res. 7:331-338 https://doi.org/10.1093/dnares/7.6.331
  10. Krieg, N.R. and J.G. Holt. 1984. Bergey's manual of systematic bacteriology. Williams and Wilkins, Baltmor
  11. Kunst, F., N. Ogasawara, I. Moszer, A.M. Albertini, G. Alloni, V. Azevedo, M.G. Bertero, P. Bessieres, A. Bolotin, and S. Borchert. 1997. The complete genome sequence of the Gram-positive bacterium Bacillus subtilis. Nature 390:249-256 https://doi.org/10.1038/36786
  12. Lahdenper, M.L., E. Simon, and J. Uoti. 1991. Mycostop-a novel biofungicide based on Streptomyces bacteria. Elsevier, Amsterdam
  13. Lechevalier, M.P. and H. Lechevalier. 1970. Chemical composition as a criterion in the classification of aerobic Actinomycetes. J. Syst. Bacteriol. 20:435-443 https://doi.org/10.1099/00207713-20-4-435
  14. Nielsen, T.H., C. Christopheresen, U. Anthoni, and J. Sorensen. 1999. Viscosinamide, a new cyclic depsipeptide with surfactant and antifungal properties produced by Pseudomonas fluorescens DR 54. J. Appl. Microbiol. 87:80-90 https://doi.org/10.1046/j.1365-2672.1999.00798.x
  15. Nielsen, T.H., C. Thrane, C. Christophersen, U. Anthoni, and J. Sorensen. 2000. Structure, production characteristics and fungal antagonism of tensin-a new antifungal cyclic lipopeptide from Pseudomonas fluorescens strain. J. Appl. Microbiol. 89:992-1001 https://doi.org/10.1046/j.1365-2672.2000.01201.x
  16. Nowak, T., N.N. Chaney, J.S. Wing, S.J. Gould, and J.E. Loper. 1999. Characterization of the pyoluteorin biosynthetic gene cluster of Pseudomonas fluorescens Pf-5. J. Bacteriol. 181:2166-2174
  17. Sabaratnam, S., A, James, and E. Traquair. 2002. Formulation of a Streptomyces biocontrol agent for the suppression of Rhizoctonia damping-off in tomato transplants. Biol. Control. 23:245-253 https://doi.org/10.1006/bcon.2001.1014
  18. Shirling, E.B. and D. Gottlieb. 1966. Methods for characterization of Streptomyces species. J. Syst. Bacteriol. 16:313-340 https://doi.org/10.1099/00207713-16-3-313
  19. Stohl, E.A., J.L. Milner, and J. Handelsman. 1999. Zwittermicin a biosynthetic cluster. Gene. 237:403-411 https://doi.org/10.1016/S0378-1119(99)00315-7
  20. Stover, C.K., X.Q. Pham, A.L. Erwin, S.D. Mizoguchi, P. Warrener, M.J. Hickey, F.S. Brinkman, W.O. Hufnagle, D.J. Kowalik, M. Lagrou. 2000. Complete genome sequence of Pseudomonas aeruginosa PA01. an opportunistic pathogen. Nature. 406:959-964 https://doi.org/10.1038/35023079
  21. Thomashow, L.S. and D.M. Weller. 1996. Current concepts in the use of introduced bacteria for biological control: mechanisms and antifungal metabolites. Chapman and Hall, New York