Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Development and Evaluation of an Immunochromatographic Assay for Screening Listeria spp. in Pork and Milk

  • Kim, Seong-Hee (National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry) ;
  • Kim, Jin-Young (Jeju Provincial Institute for Live Stock Promotion) ;
  • Han, Woong (ViroMed Co., Ltd.) ;
  • Jung, Byeong-Yeal (National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry) ;
  • Chuong, Pham-Due (Faculty of Animal Science and Veterinary Medicine, Thainguyen University of Agriculture and Forestry) ;
  • Joo, Hae-Jin (Department of Veterinary Medicine, Cheju National University) ;
  • Ba, Hoa-Van (Department of Veterinary Medicine, Cheju National University) ;
  • Son, Won-Geun (Department of Veterinary Medicine, Cheju National University) ;
  • Jee, Young-Heun (Department of Veterinary Medicine, Cheju National University) ;
  • Yoon, Byoung-Su (Department of Biology, Kyonggi University) ;
  • Lee, Yong-Soon (Department of Veterinary Medicine, Seoul National University) ;
  • Lim, Yoon-Kyu (Department of Veterinary Medicine, Cheju National University)
  • Published : 2007.08.31
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Abstract

Rapid immunochromatographic assay (ICA) kits were developed using flagella-specific monoclonal antibodies (MAbs) and rabbit polyclonal antibodies for screening Listeria spp. in food. The establishment of different formats, MAb 2B1 as capture antibody and MAb 7A3 or rabbit polyclonal antibodies as detector antibody, was compared. The 2 formats of the ICA kit were shown to have specific reactions with Listeria and no cross-reactivity with any of the non-Listeria including Escherichia coli O157:H7 and Salmonella enteritidis. The detection limits of the ICA kit using the combination of gold-labeled MAb 7A3 and MAb 2B1 showed $1{\times}10^5$ and $1{\times}10^6\;CFU/0.1\;mL$ at 22 and $30^{\circ}C$, respectively. The other format of the ICA kit using the combination of gold-labeled rabbit polyclonal antibodies and MAb 2B1 showed $1{\times}10^6\;CFU/0.1\;mL$ at $22^{\circ}C$ but weak signal at 30 culture. The format utilizing MAb was more sensitive than the one using polyclonal antibodies for capture antibody. Samples contaminated with L. monocytogenes 4b culture (9-10, 5-6, and 1-2 CFU/mL) on pork and pasteurized milk were confirmed as positive results. Current data suggests that this ICA kit is a rapid, simple and effective tool to screen for Listeria spp. in food.

Keywords

References

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