Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Optimal Conditions for the Embryonic Development of Sea Urchin, Strongylocentrotus intermedius for Using the Bioassay

북쪽말똥성게, Strongylcentrotus intermedius를 이용한 생물검정 최적 발생조건

  • 류태권 (한국해양연구원 남해연구소) ;
  • 성찬경 (㈜네오엔비즈 환경안전연구소) ;
  • 한기명 (한국해양연구원 남해연구소) ;
  • 황인영 (인제대학교 자연과학대학 환경공학부) ;
  • 이택견 (한국해양연구원 남해연구소) ;
  • 이창훈 (㈜네오엔비즈 환경안전연구소)
  • Published : 2007.09.30
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Abstract

Even though some standard developmental bioassay protocols for environmental assessment using sea urchins have already been described, there have not been many attempts to apply and modify these protocols with Korean species. Therefore, there is a strong need to establish standard bioassay protocols using sea urchins commonly found in Korea. Prior to developing a new protocol, it is essential to know the optimal conditions for the bioassay procedures. We investigated the optimal conditions (temperature, salinity, and embryo density) of the sea urchin Strongylocentrotus intermedius. The ideal temperature for developmental bioassay of S. intermedius was determined to be $15^{\circ}C$ and the time required for the embryo to become pluteus larva was 72 hr. The optimal range of salinity for the embryo toxicity using S. intermedius was between 30 to 32 psu, which is similar to the range found in the natural habitats of adult populations. The optimum density of embryos at the beginning of bioassays was 100 embryos/mL. When the assays were carried out at higher densities, the proportion of normally developed larvae decreased significantly.

Keywords

References

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