Journal of Microbiology and Biotechnology

  • 제17권8호
  • /
  • Pages.1390-1393
  • /
  • 2007
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Analysis of the Stress Effects of Endocrine Disrupting Chemicals (EDCs) on Escherichia coli

  • Kim, Yeon-Seok (College of Life Sciences and Biotechnology, Korea University) ;
  • Min, Ji-Ho (Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST)) ;
  • Hong, Han-Na (Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST)) ;
  • Park, Ji-Hyun (Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST)) ;
  • Park, Kyeong-Seo (Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST)) ;
  • Gu, Man-Bock (College of Life Sciences and Biotechnology, Korea University)
  • 발행 : 2007.08.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

In this study, three of the representative EDCs, $17{\beta}$-estradiol, bisphenol A, and styrene, were employed to find their mode of toxic actions in E. coli. To accomplish this, four different stress response genes, recA, katG, fabA, and grpE genes, were used as a representative for DNA, oxidative, membrane, or protein damage, respectively. The expression levels of these four genes were quantified using a real-time RT-PCR after challenge with three different EDCs individually. Bisphenol A and styrene caused high-level expression of recA and katG genes, respectively, whereas $17{\beta}$-estradiol made no significant changes in expression of any of those genes. These results lead to the classification of the mode of toxic actions of EDCs on E. coli.

키워드

참고문헌

  1. Alberto, M. 2002. Hazard identification and risk assessment of endocrine disrupting chemicals with regard to developmental effects. Toxicology 27: 367-370
  2. Barbu, V. and F. Dautry. 1989. Northern blot normalization with a 28S rRNA oligonucleotide probe. Nucleic Acids Res. 17: 7115 https://doi.org/10.1093/nar/17.17.7115
  3. Bromberg, R., S. M. George, and M. W. Peck. 1998. Oxygen sensitivity of heated cells of Escherichia coli O157:H7. J. Appl. Microbiol. 85: 231-237 https://doi.org/10.1046/j.1365-2672.1998.00482.x
  4. Bustin, S. A. 2000. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J. Mol. Endocrinol. 25: 169-193 https://doi.org/10.1677/jme.0.0250169
  5. Cooper, R. L. and R. J. Kavlock. 2001. Determining indicators of exposure and effects for endocrine disrupting chemicals (EDCs): An introduction. Human Ecol. Risk Assess. 7: 971-978 https://doi.org/10.1080/20018091094790
  6. Demple, B. 1991. Regulation of bacterial oxidative stress genes. Annu. Rev. Gen. 25: 315-337 https://doi.org/10.1146/annurev.ge.25.120191.001531
  7. Dhar, A. K., M. M. Roux, and K. R. Klimpel. 2002. Quantitative assay for measuring the Taura syndrome virus (TSV) and yellow head virus (YHV) load in shrimp by realtime RT-PCR using SYBR Green chemistry. J. Virol. Methods 104: 69-82 https://doi.org/10.1016/S0166-0934(02)00042-3
  8. Gu, M. B., J. Min, and R. A. LaRossa. 2000. Bacterial bioluminescent emission from recombinant Escherichia coli harboring a recA::luxCDABE fusion. J. Biochem. Biophys. Methods 45: 45-56 https://doi.org/10.1016/S0165-022X(00)00100-7
  9. Gu, M. B., J. Min, and E. J. Kim. 2002. Toxicity monitoring and classification of endocrine disrupting chemicals (EDCs) using recombinant bioluminescent bacteria. Chemosphere 46: 289-294 https://doi.org/10.1016/S0045-6535(01)00081-9
  10. Jung, C. Y., J. Y. Lee, and J. Kim. 2005. DNA repair activity of human rpS3 is operative to genotoxic damage in bacteria. J. Microbiol. Biotechnol. 15: 484-490
  11. Jung, S. J., H. J. Kim, and H. Y. Kim. 2005. Quantitative detection of Salmonella typhimurium contamination in milk, using real-time PCR. J. Microbiol. Biotechnol. 15: 1353- 1358
  12. Kang, Y.-S., Y. J. Kim, C. O. Jeon, and W. Park. 2006. Characterization of naphthalene-degrading pseudomonas species isolated from pollutant-contaminated sites: Oxidative stress during their growth on naphthalene. J. Microbiol. Biotechnol. 16: 1819-1825
  13. Kim, B. C. and M. B. Gu. 2006. Expression analysis of stress-specific responsive genes in two-stage continuous cultures of Escherichia coli using cDNA microarray and real-time RT-PCR analysis. Enzyme Microb. Technol. 39: 440-446 https://doi.org/10.1016/j.enzmictec.2005.11.032
  14. Kim, J.-H., H.-S. Song, D.-H. Kim, and H.-Y. Kim. 2006. Quantification of genetically modified canola GT73 using TaqMan real-time PCR. J. Microbiol. Biotechnol. 16: 1778- 1783
  15. Kim, Y. S., J. H. Min, H. N. Hong, J. H. Park, K. S. Park, and M. B. Gu. 2007. Gene expression analysis and classification of mode of toxicity of polycyclic aromatic hydrocarbons (PAHs) in Escherichia coli. Chemosphere 66: 1243-1248 https://doi.org/10.1016/j.chemosphere.2006.07.040
  16. Lee, H. J. and M. B. Gu. 2003. Effects of benzo[a]pyrene on genes related to the cell cycle and cytochrome P450 of Saccharomyces cerevisiae. J. Microbiol. Biotechnol. 13: 624-627
  17. Lee, H. J., J. Villaume, D. C. Cullen, B. C. Kim, and M. B. Gu. 2003. Monitoring and classification of PAH toxicity using an immobilized bioluminescent bacteria. Biosens. Bioelectron. 18: 571-577 https://doi.org/10.1016/S0956-5663(03)00039-3
  18. Liu, W. and D. A. Saint. 2002. A new quantitative method of real time reverse transcription polymerase chain reaction assay based on simulation of polymerase chain reaction kinetics. Anal. Biochem. 302: 52-59 https://doi.org/10.1006/abio.2001.5530
  19. Livak, K. J. and T. D. Schmittgen. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25: 402-408 https://doi.org/10.1006/meth.2001.1262
  20. Mager, W. H. and A. J. J. Kruijff. 1995. Stress-induced transcriptional activation. Microbiol. Rev. 59: 506-531
  21. Min, J. and M. B. Gu. 2004. Adaptive responses of Escherichia coli for oxidative and protein damage using bioluminescence reporters. J. Microbiol. Biotechnol. 14: 466-469
  22. Mohamed, A. A., R. Alary, P. Joudrier, and M. Gautier. 2005. Comparative expression of five Lea genes during wheat seed development and in response to abiotic stresses by real-time quantitative RT-PCR. Biochim. Biophys. Acta 1730: 56-65 https://doi.org/10.1016/j.bbaexp.2005.05.011
  23. Peters, I. R., C. R. Helps, E. J. Hall, and M. J. Day. 2004. Real-time RT-PCR: Considerations for efficient and sensitive assay design. J. Immunol. Methods 286: 203-217 https://doi.org/10.1016/j.jim.2004.01.003
  24. Pomposiello, P. J. and B. Demple. 2001. Redox-operated genetic switches: The SoxR and OxyR transcriptional factors. Trends Biotechnol. 19: 109-114 https://doi.org/10.1016/S0167-7799(00)01542-0