Development of Extruded Pellet for Growth of Flounder (Paralichthys olivaceus) in Commercial Scale Feeding Trials

넙치 육성용 배합사료 개발을 위한 현장 사육 실험

  • Seo, Joo-Young (Faculty of Marine Bioscience and Technology, Kangnung National University) ;
  • Choi, Jin (Faculty of Marine Bioscience and Technology, Kangnung National University) ;
  • Lee, Jong-Ha (Eastsea Mariculture Research Institute, National Fisheries Research and Development Institute) ;
  • Lee, Sang-Min (Faculty of Marine Bioscience and Technology, Kangnung National University)
  • 서주영 (강릉대학교 해양생명공학부) ;
  • 최진 (강릉대학교 해양생명공학부) ;
  • 이종하 (국립수산과학원 동해특성화연구센터) ;
  • 이상민 (강릉대학교 해양생명공학부)
  • Published : 2007.05.25

Abstract

Two feeding experiments were conducted to evaluate the effects of the experimental extruded pellet (EP), commercial EP and raw fish-based moist pellet (MP) on growth of growing flounder (Paralichthys olivaceus). In experiment 1, two replicate groups of the fish (average body weight of $115{\pm}3.2\;g$) were fed one of five experimental diets (EP1-EP5) and MP in circular concrete tanks (4.6 m $\phi$, 0.6 m depth) for 78 days. In experiment 2, in order to evaluate EP in the commercial scale ($8\;m\;{\times}\;8\;m$), flounder (average body weight of $137{\pm}11.4\;g$) were fed one of five EPs (EP1-EP5) used in experiment 1, commercial EP (EP6) and two MEPs (MEP4 and MEP6) as moist pellet types which were made from EP4 and EP6, respectively, by being sprayed with a mixture of water and additives for 80 days. In experiment 1, weight gain of fish fed the all EPs was not significantly different from that of MP. Feed efficiency of fish fed the EP4 was highest, and protein efficiency ratio of fish fed the EP4 and EP5 was significantly higher than that of fish fed the MP (P<0.05). Significant differences were observed in the contents of moisture and crude lipid in the muscle, and moisture in the liver of fish (P<0.05). In experiment 2, weight gain of fish fed the EP4 and MEP4 were highest and feed efficiency showed high tendency in EP4, MEP4 and MEP6. Fish fed the MEP6 showed higher weight gain and feed efficiency compared to those of fish fed the EP6, but there was no difference in those of fish fed EP4 and MEP4. Based on the results of this study, dietary formulations used in EPs could be most recommendable applied in the practical extruded pellet feeds for flounder grown from 114 g to 350 g.

육성기 넙치 사육용으로 부상배합사료 및 생사료의 사육효과를 비교하기 위하여 2회에 걸친 사육실험을 실시하였다. 실험 1에서는 평균체중 115 g의 넙치를 5종류의 실험EP ($EP1{\sim}EP5$v와 생사료(MP)로 78일간 사육 실험하였으며, 실험 2는 양식현장에서 평균체중 137 g의 넙치를 실험 1에 사용한 5종류의 EP ($EP1{\sim}EP5$), 시판사료(EP6) 및 EP4와 EP6에 물과 각종 영양소를 흡착시킨 2종류 흡착-습사료(MEP4 및 MEP6)로 80일간 사육 실험하였다. 사육실험 결과, 실험 1에서 증중율은 모든 EP 실험구가 MP 실험구와 유의한 차이가 없었으며, EP4는 MP에 비하여 더 높은 값을 보였다. 사료효율은 EP4 실험구가 다른 실험구보다 유의적으로 높은 결과를 보였다(P<0.05). 등근육의 간과 수분함량 및 간의 지질함량은 실험구간에 유의한 차이를 보였다. 실험 2에서는 생존율은 $89{\sim}99%$의 범위로 양호한 결과를 보였다. 증중량은 EP4와 MEP4 실험구가 가장 높은 값을 보였으며, 그 다음으로 EP3과 MEP6 실험구가 양호한 결과를 보였다. 사료효율은 모든 실험구에서 108% 이상으로 양호하였으며, EP4, MEP4 및 MEP6에서 높은 값을 보였다. 이상의 결과들로부터, $114{\sim}350\;g$인 넙치에게 생사료를 기초로 제조된 MP를 공급하는 것 보다 영양소 균형이 잘 갖춰진 EP를 공급하여도 좋을 것으로 보이며, EP 공급시 물과 다른 영양소의 흡착과정은 불필요할 것으로 판단된다. 또한, MP와 상업용 EP 실험구에 비해 양호한 성장 결과를 보인 EP4는 MP 대체 실용사료로서 사용될 수 있을 것으로 기대된다.

Keywords

References

  1. AOAC. 1990. Official Methods of Analysis. 15th edition. Association of Official Analytical Chemists. Arlington, Virginia. 1298 pp
  2. Cho, S. H., S.-M. Lee and J. H. Lee. 2005. Effects of the extruded pellets and raw fish-based moist pellet on growth and body composition of flounder Paralichthys olivaceus L. for 10 months. J. Aquacult., 18, 60-65
  3. Duncan, D. B., 1955. Multiple-range and multiple F tests. Biometrics, 11, 1-42 https://doi.org/10.2307/3001478
  4. Jang, H.-S., K.-D. Kim and S.-M. Lee, 2005. Effect of various commercial fish meals as dietary protein sources on growth and body composition of juvenile flounder, Paralichthys olivaceus. J. Aquacult., 18, 267-271
  5. Kikuchi, K., 1999. Use of defatted soybean meal as a substitute for fish meal in diets of Japanese flounder (Paralichthys olivaceus). Aquaculture, 179, 3-11 https://doi.org/10.1016/S0044-8486(99)00147-7
  6. Kim, G.-U., H.-S. Jang, J.-Y. Seo and S.-M. Lee, 2005. Effect of feeding frequency of extruded pellet on growth and body composition of juvenile flounder, Paralichthys olivaceus during the winter season. J. Aquacult., 18, 31-36
  7. Kim, K.-D. and S.-M. Lee, 2004. Requirement of dietary n-3 highly unsaturated fatty acids for juvenile flounder (Paralichthys olivaceus). Aquaculture, 229, 315-323 https://doi.org/10.1016/S0044-8486(03)00356-9
  8. Kim, K. W., X. J. Wang, S. M. Choi, G. J. Park and S. C. Bai. 2004, Evaluation of optimum dietary protein-to-energy ratio in juvenile olive flounder, Paralichthys olivaceus. Aquacult. Res., 35, 250-255 https://doi.org/10.1111/j.1365-2109.2004.01003.x
  9. Kim, K.-W., Y. J. Kang, H. Y. Lee, K.-D. Kim, S.-M. Choi, S. C. Bai and H.-S. Park, 2006. Commercial scale evaluation of practical extruded pellet feed for the olive flounder (paralichthys olivaceus). J. Kor. Fish. Soc., 39, 100-105 https://doi.org/10.5657/kfas.2006.39.2.100
  10. Kim, S. M., S.-M. Lee and B.-D. Yoon, 2003. Effect of fermented food garbage in diet on growth and body composition of juvenile flounder (Paralichthys olivaceus). J. Fish. Sci. Tech., 6, 45-50 https://doi.org/10.5657/fas.2003.6.2.045
  11. Kim, Y.-U., 2005. Policy of artificial feed supply for marine fish culture in Korea. International Symposium on the Present Status of Nutrition Research and the Future of Aquaculture Feed in Korea. NFRDI, Busan, Korea, 12 August, 2005. pp. 11-16
  12. Lee, S.-M., C. H. Seo and Y. S. Cho. 1999. Growth of the juvenile olive flounder (Paralichthys olivaceus) fed the diets at different feeding frequencies. J. Kor. Fish. Soc., 32, 18-21
  13. Lee, S.-M., C. S. Park and I. C. Bang. 2002. Dietary protein requirement of young Japanese flounder Paralichthys olivaceus fed isocaloric diets. Fish. Sci., 68, 158-164 https://doi.org/10.1046/j.1444-2906.2002.00402.x
  14. Lee, S.-M., K.-D. Kim and S. P. Lall, 2003. Utilization of glucose, maltose, dextrin and cellulose by juvenile flounder (Paralichthys olivaceus). Aquaculture, 221, 427-438 https://doi.org/10.1016/S0044-8486(03)00061-9
  15. Lee, S.-M., S. H. Cho and K. D. Kim. 2000. Effects of dietary protein and energy levels on growth and body composition of juvenile flounder Paralichthys olivaceus. J. World Aquacult. Soc., 31, 306-315
  16. Lee, S.-M., J.-Y. Seo, Y.-W. Lee, K.-D. Kim, J. H. Lee and H.-S. Jang, 2005. Evaluation of experimental extruded pellet, commercial pellet and raw fish-based moist pellet for growing flounder, Paralichthys olivaceus. J. Aquacult., 18, 287-297
  17. Pham, M. A., K.-J. Lee, S.-J. Lim, B.-J. Lee, S.-S. Kim, Y.-J. Park and S.-M. Lee. 2005. Fish meal replacement by cottonseed and soybean meal in diets for early juvenile olive flounder, Paralichthys olivaceus. J. Aquacult., 18, 215-221
  18. Seo, J.-Y., J. H. Lee, G.-U. Kim and S.-M. Lee. 2005. Effect of extruded and moist pellets at different feeding rate on growth and body composition of juvenile flounder, Paralichthys olivaceus. J. Aquacult., 18, 26-30
  19. SPSS Inc. 1997. SPSS Base 10.0 for Window, SPSS Inc., 444 N. Michigan Avenue, Chicago, IL, USA