Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Biological Characteristics of Scuticociliate, Philasterides dicentrarchi Isolated from Cultured Olive Flounder, Paralichthys olivaceus

양식 넙치에서 분리된 스쿠티카섬모충 Philasterides dicentrarchi의 생물학적 특성

  • 진창남 (목포지방해양수산청 진도해양수산사무소) ;
  • 강현실 (제주대학교 해양과학부) ;
  • 이창훈 (국립수산과학원 제주수산연구소) ;
  • 이영돈 (제주대학교 해양과학부) ;
  • 이제희 (제주대학교 해양과학부) ;
  • 허문수 (제주대학교 해양과학부)
  • Published : 2007.05.25
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Abstract

Biological characteristics of Philasteridies dicentrarchi (Ciliophora:Scuticociliate) isolated from the cultured olive flounder Paralichthys olivaceus was determined out by culture, growth conditions, and in vitro relief effect of chemical compounds. The scuticociliate has an active propagation ability by utilizing organic matters obtained from cell strain, bacteria, assorted feed, brain tissue and rotifer tissue. The ciliate achieved population growth activity under the conditions of $5{\sim}45\;ppt$ in salinity and pH 6-9. The ciliate had survived and propagated under the water temperature ranging $10{\sim}30^{\circ}C$, but active growth was observed in the temperature ranges of $10{\sim}25^{\circ}C$. Therapeutic trials were performed with formalin and hydrogen peroxide. The extermination time of the parasites with formalin was in 30 minutes both at 300 and 400 ppm, 60 minutes at 200 ppm, 90 minutes at 100 ppm, and 120 minutes at 50 ppm, respectively. In hydrogen peroxide treatments extermination time was 60 minutes in 300 ppm, 90 minutes in 200 ppm, and 150 minutes at 150 ppm and 100 ppm concentrations.

양식넙치에서 분리된 스쿠티카섬모충 P. dicentrarchi에 대한 생물학적 특성을 규명하기 위해 배양, 생육환경, 화합물의 in vitro 구제실험 등을 실시하였다. P. dicentrarchi 배양실험 결과 세포, 세균, 넙치치어 배합사료, 뇌 조직과 로티퍼 조직 등의 유기물을 먹이로 활발히 증식하였다. 생육환경 조건은 염분농도 $5{\sim}45\;ppt,\;pH\;6{\sim}9$ 범위에서 증식이 이루어졌으며, 온도는 $10{\sim}30^{\circ}C$ 범위에서 생존 및 증식이 가능하나 $10{\sim}25^{\circ}C$ 범위에서 활발한 증식이 이루어졌다. P. dicentrarchi의 화합물 구제실험 결과 포르말린 300, 400 ppm에 30분, 200 ppm에 60분, 100 ppm에 90분, 50 ppm에서 120분 만에 구제효과를 보였다. 과산화수소 약욕에 따른 구제효과는 300 ppm에서 60분, 200 ppm에서 90분 만에 구제효과를 보였으며, 150 ppm 처리구와 100 ppm 처리구에서는 150분 만에 각각 운동성을 완전히 상실하여 구제효과를 보였다.

Keywords

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