Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Bioassay of Environment-friendly Insecticides for Management of Mosquito, Culex pipieos molestus

지하집모기 (Culex pipiens molestus)의 방제를 위한 환경친화적 살충제의 생물검정

  • Choi, Su-Yeon (Dept. Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Oh, Se-Chan (Dept. Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Cho, Min-Su (Dept. Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Paek, Seung-Kyoung (Dept. Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Jin-Su (Dept. Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Da-A (Dept. Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Gil, Mi-Ra (Dept. Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Youn, Young-Nam (Dept. Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Yu, Yong-Man (Dept. Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
  • 최수연 (충남대학교 농업생명과학대학 농생물학과) ;
  • 오세찬 (충남대학교 농업생명과학대학 농생물학과) ;
  • 조민수 (충남대학교 농업생명과학대학 농생물학과) ;
  • 백승경 (충남대학교 농업생명과학대학 농생물학과) ;
  • 김진수 (충남대학교 농업생명과학대학 농생물학과) ;
  • 김다아 (충남대학교 농업생명과학대학 농생물학과) ;
  • 길미라 (충남대학교 농업생명과학대학 농생물학과) ;
  • 윤영남 (충남대학교 농업생명과학대학 농생물학과) ;
  • 유용만 (충남대학교 농업생명과학대학 농생물학과)
  • Published : 2007.08.30
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Abstract

Bioassay of mosquito, Culex pipiens molestus, larva was investigated by several environment-friendly insecticides. These insecticides were Novaluron as chitin synthesis inhibitor, Metho-xyfenozide as ecdysone agonist, Pyriproxyfen as juvenile hormone mimic, and Spiromesifen as lipid biosynthesis inhibitor. The 50% lethal concentration $(LC_{50})$ of these insecticides were 0.00039, 0.07193, 0.65006 and 0.04839 ppm, respectively. Novaluron has lower concentration than any other insecticide. To determine the treatment time against larval stages, insecticides were applied to different larval stages of C. pipiens molestus. Mortality ratios of mosquito larva treated with Novaluron were 100.0, 84.5, 71.0 and 48.5% on 2, 4, 7 and 10 days after hatching from eggs, respectively. Otherwise, with the other insecticides, mortality ratios were under 80% with 2 days old larva. When exposure periods were tested to 3 or 4 days old larva against 4 insecticides, at least 3 hours were needed to 100% control effect against Novaluron, and over 12 hours with other insecticides.

몇 가지 환경 친화적 살충제를 사용하여 지하집모기 유충에 대한 생물활성을 조사하였다. 본 실험에 사용한 약제로는 키틴합성저해제인 Novaluron, 탈피촉진제인 Methoxyfenozide, 탈피억제제인 Pyriproxyfen 그리고 지질생합성저해제인 Spiromesifen을 사용하였다. 지하집모기의 유충에 대한 약제별 반수치사농도는 각각 0.00039, 0.07193, 0.65006 그리고 0.04839 ppm으로 키틴합성저해제인 Novaluron 가 가장 낮은 농도를 보였다. 모기 유충방제에 필요한 효과적인 약제 처리시기를 결정하기 위하여 모기 유충의 령기에 따른 약제 감수성 실험을 실시한 결과, Novaluron 은 부화 후 2 일차, 4 일차, 7 일차 및 10 일차에서 각각 100%, 84.5%, 71% 그리고 48.5% 의 방제가로 IGR 약제의 특정이 나타났다. 또한, Methoxyfenozide, Pyriproxyfen 그리고 Spiromesifen은 부화 후 2 일차에서 80% 이하의 살충효과로 Novaluron 과 비교하여 낮은 방제가를 나타냈었다. 한편 모기유충에 대한 효과적인 약제 노출시간을 알아본 결과 Novaluron은 100% 살충효과를 나타내는데 3시간이 필요하였고, 나머지 약제들은 12 시간 이상의 노출시간에서도 100% 방제효과를 나타내지 못하였다.

Keywords

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