• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2002.05a
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• pp.188-188
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• 2002

• Korean Journal of Environmental Biology
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• v.32 no.2
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• pp.112-117
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• 2014

Predation efficiency and preference of the hydrophilid water beetle, Hydrochara affinis (Coleoptera: Hydrophilidae), larvae on two mosquito species, Culex pipiens molestus and Ochlerotatus togoi, were tested under laboratory conditions. Experiments were conducted in a transparent cylindrical plastic container (diameter, 100 mm; height, 40 mm; water level, 15 mm) for 24 hours (16 h, light; 8 h, dark) at $25^{\circ}C$. The predation efficiency of H. affinis on mosquito larvae was investigated under various prey conditions (1, 2, 4, 8, 16, 32, 64, and 128 larvae) with five replicates. Further, in order to investigate the prey preference on two mosquito species, each 32 larvae of C. pipiens molestus and O. togoi were provided simultaneously for the first instar larva of H. affinis with 5 replicates. As a result, the predation curves of H. affinis on two mosquito species were logarithmic; and the number of consumed prey increased as the number of predator instars were increased. As predicted from the predation curves, the estimated maximum number of C. pipiens molestus and O. togoi consumed by a third instar larva of H. affinis per a day was 926 and 304 larvae, respectively. As predicted from the Holling's disk equation, the differences in consumed prey number between predator instars and prey species were caused by handling time rather than attack rate. Handling time decreased rapidly as the predator larvae grew, and that of O. togoi was twice longer than that of C. pipiens molestus.

• Korean journal of applied entomology
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• v.46 no.2
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• pp.261-267
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• 2007

Bioassay of mosquito, Culex pipiens molestus, larva was investigated by several environment-friendly insecticides. These insecticides were Novaluron as chitin synthesis inhibitor, Metho-xyfenozide as ecdysone agonist, Pyriproxyfen as juvenile hormone mimic, and Spiromesifen as lipid biosynthesis inhibitor. The 50% lethal concentration $(LC_{50})$ of these insecticides were 0.00039, 0.07193, 0.65006 and 0.04839 ppm, respectively. Novaluron has lower concentration than any other insecticide. To determine the treatment time against larval stages, insecticides were applied to different larval stages of C. pipiens molestus. Mortality ratios of mosquito larva treated with Novaluron were 100.0, 84.5, 71.0 and 48.5% on 2, 4, 7 and 10 days after hatching from eggs, respectively. Otherwise, with the other insecticides, mortality ratios were under 80% with 2 days old larva. When exposure periods were tested to 3 or 4 days old larva against 4 insecticides, at least 3 hours were needed to 100% control effect against Novaluron, and over 12 hours with other insecticides.

• Korean journal of applied entomology
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• v.47 no.4
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• pp.457-465
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• 2008

Eight Bacillus thuringiensis strains activated against mosquito larva were compared their characterization. Spherical-shaped parasporal inclusion of B. thuringiensis subsp. tohokuensis CAB167 was observed by phase-contrast microscopy and scanning electron microscopy. $LC_{50}$ values of B. thuringiensis subsp. tohokuensis CAB167 against Culex pipiens molestus, Culex pipiens pallens, and Aedes aegyti were 173, 190 and 580 ng/ml, respectively. B. thuringiensis subsp. tohokuensis CAB167 had a parasporal inclusion containing 4 major protein components, for example, 135, 80, 49 and 28-kDa by SDS-PAGE. Otherwise, after trypsin digestion of parasporal inclusion, SDS-PAGE was showed new protease-resistant peptides at 72 and 63-kDa. Activated toxins of isolated CAB167 were different from other reference strains on a serological by immuno-diffusion test.