Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Assessment of Lipopolysaccharide-binding Activity of Bifidobacterium and Its Relationship with Cell Surface Hydrophobicity, Autoaggregation, and Inhibition of Interleukin-8 Production

  • Park, Myeong-Soo (Research Institute, BIFIDO Co., Ltd.) ;
  • Kim, Min-Jeong (Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University) ;
  • Ji, Geun-Eog (Research Institute, BIFIDO Co., Ltd.)
  • Published : 2007.07.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to screen probiotic bifidobacteria for their ability to bind and neutralize lipopolysaccharides (LPS) from Escherichia coli and to verify the relationship between LPS-binding ability, cell surface hydrophobicity (CSH), and inhibition of LPS-induced interleukin-8 (IL-8) secretion by HT-29 cells of the various bifidobacterial strains. Ninety bifidobacteria isolates from human feces were assessed for their ability to bind fluorescein isothiocyanate (FITC)-labeled LPS from E. coli. Isolates showing 30-60% binding were designated LPS-high binding (LPS-H) and those with less than 15% binding were designated LPS-low binding (LPS-L). The CSH, autoaggregation (AA), and inhibition of LPS-induced IL-8 release from HT-29 cells of the LPS-H and LPS-L groups were evaluated. Five bifidobacteria strains showed high levels of LPS binding, CSH, AA, and inhibition of IL-8 release. However, statistically significant correlations between LPS binding, CSH, AA, and reduction of IL-8 release were not found. Although we could isolate bifidobacteria with high LPS-binding ability, CSH, AA, and inhibition of IL-8 release, each characteristic should be considered as strain dependent. Bifidobacteria with high LPS binding and inhibition of IL-8 release may be good agents for preventing inflammation by neutralizing Gram-negative endotoxins and improving intestinal health.

Keywords

References

  1. Alander, M., R. Korpela, M. Saxelin, T. Vilpponen-Salmela, T. Matilla-Sandholm, and A. Wright. 1997. Recovery of Lactobacillus rhamnosus GG from human colonic biopsies. Lett. Appl. Microbiol. 24: 361-364 https://doi.org/10.1046/j.1472-765X.1997.00140.x
  2. Bibiloni, R., R. N. Fedorak, G W. Tannock, K. L. Madsen, P. Gionchetti, M. Campieri, C. De Simone, and R. B. Sartor. 2005. VSL#3 probiotic mixture induces remission in patients with active ulcerative colitis. Am. J. Gastroenterol. 100: 1539-1546 https://doi.org/10.1111/j.1572-0241.2005.41794.x
  3. Boris, S., J. E. Suarez, and C. Barbes. 1997. Characterization of the aggregation promoting factor from Lactobacillus gasseri, a vaginal isolate. J. Appl. Microbiol. 83: 413-420 https://doi.org/10.1046/j.1365-2672.1997.00250.x
  4. Cario, E. and D. K. Podolsky. 2000. Differential alteration in intestinal epithelial cell expression of toll-like receptor 3 (TLR3) and TLR4 in inflammatory bowel disease. Infect. Immun. 68: 7010-7017 https://doi.org/10.1128/IAI.68.12.7010-7017.2000
  5. De Dooy, J. J., L. M. Mahieu, and H. P. Van Bever. 2001. The role of inflammation in the development of chronic lung disease in neonates. Eur. J. Ped. 160: 457-463 https://doi.org/10.1007/s004310100785
  6. De Man, J. C., M. Rogasa, and M. E. Sharpe. 1960. A medium for the cultivation of lactobacilli. J. Appl. Bacteriol. 23: 130-135 https://doi.org/10.1111/j.1365-2672.1960.tb00188.x
  7. Del Re, B., A. Busetto, G. Vignola, B. Sgorbati, and D. Palenzona. 1998. Autoaggregation and adhesion ability in a Bifidobacterium suis strain. Lett. Appl. Microbiol. 27: 307-310 https://doi.org/10.1046/j.1472-765X.1998.00422.x
  8. Del Re, B., B. Sgorbati, M. Miglioli, and D. Palenzona. 2000. Adhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacterium longum. Lett. Appl. Microbiol. 32: 438-442
  9. Enrica, C., G. Simone, M. Diego, T. Isabella, and P. Carlo. 2005. Condition affecting cell surface properties of human intestinal bifidobacteria. Antonie Van Leeuwenhoek 88: 207-219 https://doi.org/10.1007/s10482-005-6501-3
  10. Freter, M. 1992. Factors affecting the microecology of the gut, pp. 111-145. In R. Fuller (ed.), Probiotics. The Scientific Basis. Chapman and Hall, Glasgow
  11. Furrie, E., S. Macfarlane, A. Kennedy, J. H. Cummings, S. V. Walsh, D. A. O'Neil, and G. T. Macfarlane. 2005. Synbiotic therapy (Bifidobacterium longum/Synergy 1) initiates resolution of inflammation in patients with active ulcerative colitis: A randomised controlled pilot trial. Gut 54: 242-249 https://doi.org/10.1136/gut.2004.044834
  12. Gionchetti, P., F. Rizzello, A. Venturi, P. Brigidi, D. Matteuzzi, G. Bazzocchi, G. Poggioli, M. Miglioli, and M. Campieri. 2000. Oral bacteriotherapy as maintenance treatment in patients with chronic pouchitis: A double-blind, placebocontrolled trial. Gastroenterology 119: 305-309 https://doi.org/10.1053/gast.2000.9370
  13. Guarner, F. and J. R. Malagelada. 2003. Gut flora in health and disease. Lancet 361: 512-519 https://doi.org/10.1016/S0140-6736(03)12489-0
  14. Kim, H. Y. and G. E. Ji. 2006. Effect of viability and integrity of Bifidobacterium on suppression of allergy in mice. J. Microbiol. Biotechnol. 16: 1010-1016
  15. Kim, I. H., M. S. Park, and G. E. Ji. 2003. Characterization of adhesion of Bifidobacterium sp. BGN4 to human enterocyte-like Caco-2 cells. J. Microbiol. Biotechnol. 13: 276-281
  16. Kim, N. J. and G. E. Ji. 2006. Modulatory activity of Bifidobacterium sp. BGN4 cell fractions on immune cells. J. Microbiol. Biotechnol. 16: 584-589
  17. Kok, R. G., A. De Waal, F. Schute, G. W. Welling, G. Weeken, and K. J. Hellingwere. 1996. Specific detection and analysis of a probiotic Bifidobacterium strain in infant feces. Appl. Environ. Microbiol. 62: 3668-3672
  18. Lim, N. K., J. C. Yoo, T. R. Park, and J. S. So. 1998. The relationship between cell surface hydrophobicity (CSH) and stress tolerance in Bifidobacterium spp. Food Sci. Biotechnol. 7: 66-70
  19. Madsen, K., A. Cornish, P. Soper, C. McKaigney, H. Jijon, C. Yachimec, J. Doyle, L. Jewell, and C. De Simone. 2001. Probiotic bacteria enhance murine and human intestinal epithelial barrier function. Gastroenterology 121: 580-59l https://doi.org/10.1053/gast.2001.27224
  20. McCarthy, J., L. O'Mahony, L. O'Callaghan, B. Sheil, E. E. Vaughan, N. Fitzsimons, J. Fitzgibbon, G. C. O'Sullivan, B. Kiely, J. K. Collins, and F Shanahan. 2003. Double blind, placebo controlled trial of two probiotic strains in interleukin 10 knockout mice and mechanistic link with cytokine balance. Gut 52: 975-980 https://doi.org/10.1136/gut.52.7.975
  21. Meijssen, M. A., S. L. Brandwein, H. C. Reinecker, A. K. Bhan, and D. K. Podolsky. 1998. Alteration of gene expression by intestinal epithelial cells precedes colitis in interleukin-2-deficient mice. Am. J. Physiol. 274: 472-479 https://doi.org/10.1152/ajpcell.1998.274.2.C472
  22. Ortega-Cava, C. F, S. Ishihara, M. A. Rumi, K. Kawashima, N. Ishimura, H. Kazumori, J. Udagawa, Y. Kadowaki, and Y. Kinoshita. 2003. Strategic compartmentalization of toll-like receptor 4 in the mouse gut. J. Immunol. 170: 3977-3985 https://doi.org/10.4049/jimmunol.170.8.3977
  23. Pedersen, K. and G. W. Tannock. 1989. Colonization of the porcine gastrointestinal tract by lactobacilli. Appl. Environ. Microbiol. 55: 279-283
  24. Perez, P. E, Y. Minnaard, E. A. Disalvo, and G. L. De Antoni. 1998. Surface properties of bifidobacterial strains of human origin. Appl. Environ. Microbiol. 64: 21-26
  25. Riedel, C. U., F. Foata, D. Philippe, O. Adolfsson, B. J. Eikmanns, and S. Blum. 2006. Anti-inflammatory effects of bifidobacteria by inhibition of LPS-induced NF-$\kappa$B activation. World J. Gastroenterol. 12: 3728-3735
  26. Scardovi, V. 1986. Genus Bifidobacteria, pp. 1418-1434. In P. H. A. Sneah, N. S. Mair, M. E. Sharpe, and J. G. Holt (eds.), Bergey's Manual of Systematic Bacteriology, vol. 2, Williams and Wilkins, Baltimore
  27. Schiffrin, E. and G. Kociubinski. 2002. Endotoxin binding lactic acid bacteria and bifidobacteria. In Patent Publication Number WO 02/062360 AI
  28. Sellon, R. K., S. Tonkonogy, M. Dieleman, L. A. Schultz, W. Grenther, E. Balish, D. M. Rennick, and R. B. Sartor. 1998. Resident enteric bacteria are necessary for development of spontaneous colitis and immune system activation in interleukin-10-deficient mice. Infect. Immun. 66: 5224-5231
  29. Vinderola, C. G, M. Medici, and G Pedigon. 2004. Relationship between interaction sites in the gut, hydrophobicity, mucosal immunomodulating capacities and cell wall protein profiles in indigenous and exogenous bacteria. J. Appl. Microbiol. 96: 230-243 https://doi.org/10.1046/j.1365-2672.2004.02158.x
  30. Wadstrom, T. K., M. S. Anderson, L. Axelsson, S. Lindgren, and B. Gullman. 1987. Surface properties of lactobacilli isolate from the small intestine of pigs. J. Appl. Bacteriol. 62: 513-520 https://doi.org/10.1111/j.1365-2672.1987.tb02683.x