• Journal of Microbiology and Biotechnology
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• v.6 no.6
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• pp.456-460
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• 1996

Recombinant Pseudomonas lipase was used to study protein aggregation and adsorption upon in vitro refolding. Protein adsorption as well as aggregation was responsible for major side reactions upon in vitro refolding as a function of protein concentration. The optimal range of protein concentration was determined by the relative contribution of protein aggregation and adsorption. Above the optimal range, the yield of active lipase inversely correlated with protein aggregation, showing a competition between folding and aggregation. However, adsorption of protein rather than protein aggregation is thought to contribute as a major side reaction of the refolding process at sub-optimal concentrations at which the formation of aggregates should be more reduced. Protein aggregation was influenced by the amount of guanidine hydrochloride in the refolding solvent. The refolding temperature was a critical factor determining the extent of protein aggregation. The refolding yield was also affected by the dilution fold and dilution mode, which suggests that the refolding process might kinetically compete with the rate of mixing.

• Journal of KIISE:Databases
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• v.35 no.5
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• pp.391-399
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• 2008

RSS is the XML-based format for the syndication of web contents, and users aggregate RSS feeds with RSS feed aggregators. In order to effectively aggregate RSS feeds, an RSS aggregation policy is necessary. In this paper, we first propose an aggregation policy to minimize the number of postings being missed within an aggregation. Second, we analyze and compare our aggregation policy with existing aggregation policies. Our analysis reveals that our aggregation policy can reduce approximately 23% of the aggregation missing in comparison with the other aggregation policies while it increases only 6% of the aggregation delay.

• The KIPS Transactions:PartB
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• v.8B no.6
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• pp.669-674
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• 2001

In order to reflect the aggregation situation in the aggregation process, aggregation based on situation assessment (ASA) method was proposed in [1]. It consists of the situation assessment model (SAM) and the ASA algorithm. In the SAM, the value of parameter, p, is transformed into the nearest integer value [1]. The integer-typed output of SAM is used as input for an aggregation. The integer-typed output of SAM indicates the current degree of aggregation situation. The ASA algorithm produces at most finite several aggregation results between min and max. In the sequel, the ASA method can not properly handle the applications with the more sophisticated aggregation results between min and max. In order to solve this problem, we propose two improved ASA (I-ASA) methods. In these I-ASA methods, we allow the value of parameter of SAM to be a real number, and suggest two improved ASA algorithms to make continuous aggregation results between min and max. These I-ASA methods can handle both a precise aggregation and an approximate aggregation. Therefore, when compared to the ASA method [1], the proposed I-ASA methods have advantages in that they can handle the applications with the more sophisticated aggregation results and can be used in the more general applications for aggregations.

• Korea-Australia Rheology Journal
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• v.19 no.2
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• pp.61-66
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• 2007

The aggregation characteristics of red blood cells (RBCs) are known as important factors in the microvascular flow system, and increased RBC aggregation has been observed in various pathological diseases, such as thrombosis and myocardial infarction. This paper describes a simple microfluidic device for measuring the RBC aggregation by integrating a microfluidic slit rheometry and laser-backscattering technique. While a decreasing-pressure mechanism was applied to the microfluidic rheometry, a syllectogram (the light intensity versus time) showed an initial increase and a peak caused by the high shear stress-induced disaggregation, immediately followed by a decrease in the light intensity due to RBC aggregation. The critical shear stress (CST) corresponding to the peak intensity was examined as a new index of the RBC aggregation characteristics. The CST of RBCs increased with increasing aggregation-dominating protein (fibrinogen) in the blood plasma. The essential feature of this design was the combination of the rheometric-optic characterization of RBC aggregation with a microfluidic chip, which may potentially allow cell aggregation measurements to be easily carried out in a clinical setting.

• Journal of Korean Institute of Industrial Engineers
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• v.40 no.5
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• pp.520-525
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• 2014

When the PCF (Point Coordinated Function) MAC protocol is combined with the frame aggregation method to enhance the MAC performance in IEEE 802.11 wireless LANs, the formulae for the optimal frame aggregation level for best PCF MAC performance were derived in our previous study. We extend the formulae for the PCF protocol to derive the optimal frame aggregation level for the connectivity-based multipolling MAC protocol in IEEE 802.11 wireless LANs. By simulations, we compare the performances of IEEE 802.11 wireless LANs with the optimal and random frame aggregation levels. Compared with the random frame aggregation level, the optimal frame aggregation level significantly improves the performance of IEEE 802.11 wireless LANs.

• YAKHAK HOEJI
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• v.43 no.6
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• pp.802-808
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• 1999

Vibrio vulnificus cytolysin has been incriminated as one of the important virulence determinants in V. vulnificus infection. In the present study, the effects of Vibrio vulnificus cytolysin on platelets were examined. Vibrio vulnificus cytolysin induced platelet aggregation and increased intracellular calcium concentration ($[Ca^{2+}]_i$) of rat platelets. These effects were abolished in $Ca^{2+}-free$ buffer (2 mM EGTA). Cytolysin also potentiated ADP-and collagen-induced platelet aggregation. Lanthanum (2 mM) inhibited cytolysin-diduced platelet aggregation. However, another $Ca^{2+}$ channel blockers, verapamil ($20{\;}{\mu}M$) or mefenamic acid ($20{\;}{\mu}M$) did not block cytolysin-induced platelet aggregation. Osmotic protectants, sucrose (50 mM) and raffinose (50 nM) suppressed platelet aggregation by 35.9% and 63.4%, respectively. V. vulnificus cytolysin increased membrane conductances of platelet membranes. These results suggest that cytolysin-induced platelet aggregation is mediated via lanthanum sensitive-calcium influx which resulted from the pore formation by V. vulnificus cytolysin.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.13 no.6
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• pp.1175-1180
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• 2009

Frame Aggregation is a promissing technology for improving MAC throughput in IEEE 802.11n. In IEEE 802.11n, two frame aggregation schemes, Aggregate MSDU (A-MSDU) and Aggregation MPDU (A-MPDU), are defined. In this paper, we analyze the performance the two-level frame aggregation scheme where A-MSDU and A-MPDU are combined. We develop the analytical model for the two-level frame aggregation scheme and present numerical results on the effect of bit error rate, aggregation size, and the number of nodes.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.3 no.2
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• pp.209-218
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• 2009

In a wireless sensor network(WSN) each sensor node deals with numerous sensing data elements. For the sake of energy efficiency and network lifetime, sensing data must be handled effectively. A technique used for this is data aggregation. Sending/receiving data involves numerous steps such as MAC layer control packet handshakes and route path setup, and these steps consume energy. Because these steps are involved in all data communication, the total cost increases are related to the counts of data sent/received. Therefore, many studies have proposed sending combined data, which is known as data aggregation. Very effective methods to aggregate sensing data have been suggested, but there is no means of deciding how long the sensor node should wait for aggregation. This is a very important issue, because the wait time affects the total communication cost and data reliability. There are two types of data aggregation; the data counting method and the time waiting method. However, each has weaknesses in terms of the delay. A hybrid method can be adopted to alleviate these problems. But, it cannot provide an optimal point of aggregation. In this paper, we suggest a stochastic-based data aggregation scheme, which provides the cost(in terms of communication and delay) optimal aggregation point. We present numerical analysis and results.

• Journal of Korean Society of Water and Wastewater
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• v.27 no.5
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• pp.571-579
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• 2013

The aggregation behaviors of silver nanoparticles (AgNPs) and titanium dioxide ($TiO_2$) nanoparticles were investigated. Time-resolved dynamic light scattering (DLS) was used to study the initial aggregation of AgNPs and $TiO_2$ over a range of mono (NaCl) and divalent ($CaCl_2$) electrolyte concentrations. The effects of pH, initial concentration of NPs and natural organic matters (NOM) on the aggregation of NPs were also investigated. The aggregation of both nanoparticles showed classical Derjaguin-Landau-Verwey-Overbeek (DLVO) type behavior. Divalent electrolyte was more efficient in destabilize the AgNPs and $TiO_2$ than monovalent electrolyte. The effect of pH on the aggregation of AgNPs was not significant. But the aggregation rate of $TiO_2$ was much higher with increasing pH. Higher NPs concentration leads to faster aggregation. Natural organic matter (NOM) was found to substantially hinder the aggregation of both AgNPs and $TiO_2$. This study found that the aggregation behavior of AgNPs and $TiO_2$ are closely associated with environmental factors such as ionic strength, pH, initial concentration of NPs and NOM.

• YAKHAK HOEJI
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• v.46 no.6
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• pp.441-447
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• 2002

It has been well known that vanadium shows various physiological and pharmacological properties such as an insulin-mimetic effect. In view of the reported toxic effects there is the problems that the safety margin is narrow because of its strong toxicity, Vanadate was tested for its ability to cause blood aggregation. Although vanadate or $H_2O$$_2$ alone had little effect on platelet aggregation, treatment of vanadate and $H_2O$$_2$ together induced platelet aggregation indicated that it was occurred by pervandate or hydroxyl radical produced from the reaction of vanadate and $H_2O$$_2$. It was dependent on extracellular $Ca^{2+}$ion. Platelet aggregation caused by vanadate and $H_2O$$_2$ was inhibited by ascorbic acid, tocopherol, catalase, mannitol, and Tiron. In contrast to vanadate, vanadium yeast prepared by uptaking vanadate in yeast cells did not induce platelet aggregation in the presence of $H_2O$$_2$.>.