Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cloning and Characterization of Squalene Synthase (SQS) Gene from Ganoderma lucidum

  • Zhao, Ming-Wen (College of Life Sciences, Nanjing Agricultural University, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture) ;
  • Liang, Wan-Qi (Agro-Biotech Research Center, Agricultural Academy of Shanghai) ;
  • Zhang, Da-Bing (Agro-Biotech Research Center, Agricultural Academy of Shanghai) ;
  • Wang, Nan (Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Agriculture Genetics and Breeding of Shanghai) ;
  • Wang, Chen-Guang (Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Agriculture Genetics and Breeding of Shanghai) ;
  • Pan, Ying-Jie (College of Life Sciences, Nanjing Agricultural University, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture)
  • Published : 2007.07.31
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Abstract

This report provides the complete nucleotide sequences of the full-length cDNA encoding squalene synthase (SQS) and its genomic DNA sequence from a triterpene-producing fungus, Ganoderma lucidum. The cDNA of the squalene synthase (SQS) (GenBank Accession Number: DQ494674) was found to contain an open reading frame (ORF) of 1,404 bp encoding a 468-amino-acid polypeptide, whereas the SQS genomic DNA sequence (GenBank Accession Number: DQ494675) consisted of 1,984 bp and contained four exons and three introns. Only one gene copy was present in the G. lucidum genome. The deduced amino acid sequence of Ganoderma lucidum squalene synthase (GI-SQS) exhibited a high homology with other fungal squalene synthase genes and contained six conserved domains. A phylogenetic analysis revealed that G. lucidum SQS belonged to the fungi SQS group, and was more closely related to the SQS of U. maydis than to those of other fungi. A gene expression analysis showed that the expression level was relatively low in mycelia incubated for 12 days, increased after 14 to 20 days of incubation, and reached a relatively high level in the mushroom primordia. Functional complementation of GI-SQS in a SQS-deficient strain of Saccharomyces cerevisiae confirmed that the cloned cDNA encoded a squalene synthase.

Keywords

References

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