Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
권호 표지

The Production of Methyl Trans-cinnamate in the Submerged Cultures of Tricholoma matsutake Mycelia

  • Park, Youngki (Div. Special Purpose Tree, Korea Forest Research Institute) ;
  • Lee, Wi Young (Div. Biotechnology, Korea Forest Reseach Institute) ;
  • Ahn, Jin Kwon (Div. Biotechnology, Korea Forest Reseach Institute)
  • Received : 2007.05.25
  • Accepted : 2007.06.29
  • Published : 2007.09.25
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Abstract

Methyl trans-cinnamate is a significant flavor compound of Tricholoma matsutake. Attempts were made to produce this compound by culturing the mycelium using submerged culture. No methyl trans-cinnamate could be detected when the mycelium was cultured in the basal liquid medium. However, the addition of Pinus desiflora extracts to the medium, methyl trans-cinnamate was largely produced. To find out compounds or fractions inducing methyl trans-cinnamate, dichloromethane fraction obtained from the wood extracts of P. densiflora was subjected to column chromatography. Three sub-fractions were obtained from the $CH_2CI_2$ fraction. Submerged cultured mycelium treated with sub-fraction 1 has the highest content of methyl trans-cinnamate. Maximum methyl trans-cinnamate $(470.2{\mu}g/g)$ was obtained when the first sub-fraction of dichloromethane fraction of the P. densiflora wood extracts was added to the medium. This indicates that wood extracts of P. densiflora contains inducer of the methyl trans-cinnamate production in the T. matsutake submerged culture.

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References

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