Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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Characterization of β-glucosidase from Brown Rot Fungus, Laetiporus sulphureus

  • Lee, Jae-Won (Dept. of Forest Science, College of Agriculture & Life Sciences, Seoul National University) ;
  • Park, Jun-Yeong (Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Gwak, Ki-Seob (Dept. of Forest Science, College of Agriculture & Life Sciences, Seoul National University) ;
  • Koo, Bon-Wook (Dept. of Forest Science, College of Agriculture & Life Sciences, Seoul National University) ;
  • Choi, In-Gyu (Dept. of Forest Science, College of Agriculture & Life Sciences, Seoul National University)
  • Received : 2007.06.27
  • Accepted : 2007.08.06
  • Published : 2007.09.25
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Abstract

$\beta$-Glucosidase from Laetiporus sulphureus among the enzymes related to lignocellulosic biomass degradation to sugars for using alternative bioethanol production was characterized. The highest activity of $\beta$-glucosidase was obtained on cellobiose at shaking culture. For the characterization and purification of $\beta$-glucosidase culture solution was concentrated and then purified by FPLC using ion exchange and size exclusion column. According to the results of SDS-PAGE, native PAGE and microfluidic system of purified enzyme, protein band was observed at about 132 kDa. Optimal pH and temperature of purified $\beta$-glucosi-dase were 5.0 and $60^{\circ}C$, respectively. In the kinetic properties of $\beta$-glucosidase on various substrates such as sophorose, gentiobiose and cellobiose, $K_m$ was 0.81, 1.07 and 1.70 mM, respectively.

Keywords

References

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