Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Artificial Induction of Lactation in 5 Month Old Transgenic Female Calf

생후 5개월된 형질전환 암송아지에서의 인공 비유

  • Kim, Young-Hun (College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Chang-Woo (College of Veterinary Medicine, Seoul National University) ;
  • Han, Yong-Mahn (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
  • Lee, Kyung-Kwang (Center of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology) ;
  • Cho, Jong-Ki (College of Veterinary Medicine, Chungnam National University) ;
  • Shin, Sang-Tae (College of Veterinary Medicine, Chungnam National University)
  • 김영훈 (충남대학교 수의과대학) ;
  • 이창우 (서울대학교 수의과대학) ;
  • 한용만 (한국과학기술원 생명과학과) ;
  • 이경광 (한국생명공학연구원 동물발생공학연구실) ;
  • 조종기 (충남대학교 수의과대학) ;
  • 신상태 (충남대학교 수의과대학)
  • Published : 2007.06.30
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Abstract

The purpose of this study was premature induction of lactation in the transgenic calf to confirm the expression of human lactoferrin gene in the milk as early as possible. We performed the induction of lactation in 6 normal 1-month old calves and 3 normal and 1 transgenic 5-month old calves. In order to induction of lactation, 1-month old calf was injected with estradiol benzoate (0.2 mg/kg), $17-{\beta}$ estradiol cypionate (0.1 mg/kg) and progesterone (0.25-0.5 mg/kg) in every other day by 21 days. After 10 days cessation of administration, dexamethasone (0.028 mg/kg) was injected intramuscularly, however, lactation was failed. In the 5-month old calves, estradiol benzoate (0.2 mg/kg) and progesterone (0.5 mg/kg) were injected individually every other day during 21 days. After 10 days cessation of administration, dexamethasone (0.1 mg/kg) was injected intramuscularly and milk was lactated in the next day. Lactation was successfully induced in the 5-month old normal and transgenic calves and we confirmed the lactoferrin gene in the milk of transgenic calf. In conclusion, confirmation of human lactoferrin gene expressed in the milk of the transgenic calf was possible in 20 months earlier than in normal condition.

Keywords

References

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