한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제24권2호
  • /
  • Pages.94-98
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  • 2007
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

돼지의 신장에서 Antioxidant Vitamins에 의한 허혈 및 재관류 손상의 감소에 관한 연구

Attenuation of Renal Ischemia-Reperfusion Injury by Antioxidant Vitamins in Pigs

  • 김명진 (충남대학교 수의과대학) ;
  • 이수진 (충남대학교 수의과대학) ;
  • 박창식 (충남대학교 동물자원학부, 형질전환복제돼지연구센터) ;
  • 손화영 (충남대학교 수의과대학) ;
  • 전무형 (충남대학교 수의과대학) ;
  • 정성목 (충남대학교 수의과대학) ;
  • 김명철 (충남대학교 수의과대학)
  • Kim, Myung-Jin (College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Soo-Jin (College of Veterinary Medicine, Chungnam National University) ;
  • Park, Chang-Sik (Division of Animal Science & Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Son, Hwa-Young (College of Veterinary Medicine, Chungnam National University) ;
  • Jun, Moo-Hyung (College of Veterinary Medicine, Chungnam National University) ;
  • Jeong, Seong-Mok (College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Myung-Cheol (College of Veterinary Medicine, Chungnam National University)
  • 발행 : 2007.06.30
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초록

돼지에서 신장의 허혈-재관류 손상의 감소에 대한 비타민 C와 E의 영향을 알아보기 위하여 본 연구를 실시하였다. 돼지 10두를 2개의 군으로 구분하여, 60분 동안 체온과 같은 허혈을 한쪽 신장에 유발하고 반대쪽 신장은 절제하였다. 처치군은 수술 2일전 비타민 C와 E를 이틀 동안 전처치하고, 그 뒤에 수술 중 비타민 C와 heparin이 첨가된 생리식염수를 관주-흡인하였다. 대조군은 heparin이 첨가된 생리식염수의 관주-흡인만을 하였다. Blood urea nitrogen, creatinine 및 antioxidant superoxide dismutase (SOD)를 측정하기 위하여 수술 전, 수술 후 1, 3, 7, 14일에 혈액 샘플을 채취하였다. 수술 후 14일에 안락사를 시키고, 병리조직검사를 위하여 신장을 적출하였다. BUN은 대조군과 처치군 사이에 수술 후 1일, 3일 또는 7일에 유의성이 있는 차이가 인정되었다. (p<0.05). Creatinine은 대조군과 처치군 사이에 수술 후 3일에 유의성이 있는 차이가 인정되었다. (p<0.05). 혈장에서의 항산화 효소의 활성은 대조군과 처치군 사이에서 수술 후 14일에 유의성이 있는 차이가 인정되었다. (p<0.05). 병리조직 검사 결과에서 처치군이 대조군 보다 더 적은 신장 세뇨관에서의 손상의 정도를 보였다. 비타민 C와 E는 돼지에서 신장의 허혈-재관류 손상을 감소시켰다.

This study was to investigate the effects of ascorbic acid and alpha-tocopherol on the attenuation of renal ischemia-reperfusion (IR) injury in pigs. Ten pigs were subjected to 60 minutes of warm unilateral renal ischemia followed by removal of contralateral kidney and then divided into two groups. Treatment group was performed ascorbic acid and alpha-tocopherol pretreatment 2 days before operation and ascorbic acid with heparin-saline solution irrigation-aspiration. Otherwise, control group used only irrigation-aspiration of heparin-saline solution. Blood samples were collected from these pigs for measurement of serum blood urea nitrogen (BUN) and creatinine values, antioxidant superoxide dismutase (SOD) at pre, day 1, day 3, day 7 and day 14. The kidneys were taken for histopathologic evaluation after euthanasia on postoperative day 14. The levels of BUN were significantly increased in the control group on day 1, day 3 and day 7 (P<0.05). And the level of creatinine was significantly increased in the control group on day 3 (p<0.05). Activity of antioxidant enzymes in plasma revealed significant difference (p<0.05) between control and treatment group at day 14. In histopathologic findings, treatment group was showed less damage than that of control group on the basis of renal tubular damage. It was concluded that ascorbic acid and alpha-tocopherol attenuated renal I/R injury in the pigs.

키워드

참고문헌

  1. Baud L, Ardaillou R. Involvement of reactive oxygen species in kidney damage. Br Med Bull 1993; 49: 621-629 https://doi.org/10.1093/oxfordjournals.bmb.a072635
  2. Bildik A, Kargin F, Seyrek K, Pasa S, Ozensoy S. Oxidative stress and non-enzymatic antioxidative status in dogs with visceral Leishmaniasis. Res Vet Sci 2004; 77: 63-66 https://doi.org/10.1016/j.rvsc.2004.01.005
  3. Block G, Henson DE, Levine M. Vitamin C: a new look. Ann Intern Med 1991; 114: 909-910 https://doi.org/10.7326/0003-4819-114-10-909
  4. Boaz M, Smetana S, Weinstein T, Matas Z, Gafter U, Laina A, Knecht A, Weissgarten Y, Brunner D, Fainaru M, Green MS. Secondary prevention with antioxidants of cardiovascular disease in endstage renal disease (SPACE): randomized placebo-controlled trial. Lancet 2000; 356: 1213-1218
  5. Brezis M, Rosen S, Silva P, Epstein FH. Selective vulnerability of the medullary thick ascending limb to anoxia in the isolated perfused rat kidney. J Clin Invest 1984; 73: 182-190 https://doi.org/10.1172/JCI111189
  6. Bryan CF, Luger AM, Martinez J, Muruve N, Nelson PW, Pierce GE, Ross G, Shield CF 3rd, Warady BA, Aeder MI, Helling TS. Cold ischemia time: an independent predictor of increased HLA class I antibody production after rejection of a primary cadaveric renal allograft. Transplantation 2001; 71: 875-879 https://doi.org/10.1097/00007890-200104150-00009
  7. Bulkley GB. Free radical-mediated reperfusion injury: a selective review. Br J Cancer Suppl 1987; 8: 66-73
  8. Chade AR, Rodriguez-Porcel M, Herrmann J, Zhu X, Grande JP, Napoli C, Lerman A, Lerman LO. Antioxidant Intervention Blunts Renal Injury in Experimental Renovascular Disease. J Am Soc Nephrol 2004; 15: 958-966 https://doi.org/10.1097/01.ASN.0000117774.83396.E9
  9. Clarkson PM, Thompson HS. Antioxidants: what role do they play in physical activity and health? Am J Clin Nutr 2000; 72: 637S-646S https://doi.org/10.1093/ajcn/72.2.637S
  10. Frei B, England L, Ames BN. Ascorbate is an outstanding antioxidant in human blood plasma. Proc Natl Acad Sci USA 1989; 86: 6377-6381 https://doi.org/10.1073/pnas.86.16.6377
  11. Giovannini L, Migliori M, Longoni BM, Das OK, Bertelli AA, Panichi V, Filippi C, Bertelli A. Resveratrol, a polyphenol found in wine, reduces ischemia reperfusion injury in rat kidneys. J Cardiovasc Pharmacol 2001; 37: 262-270 https://doi.org/10.1097/00005344-200103000-00004
  12. Gurel A, Armutcu F, Sahin S, Sogut S, Ozyurt H, Gulec M, Kut!u N 0, Akyol O. Protective role of alpha-tocopherol and caffeic acid phenethyl ester on ischemia-reperfusion injury via nitric oxide and myeloperoxidase in rat kidneys. Clin Chim Acta 2004; 339: 33-41 https://doi.org/10.1016/j.cccn.2003.09.013
  13. Jefayri MK, Grace PA, Mathie RT. Attenuation of reperfusion injury by renal ischaemic preconditioning: the role of nitric oxide. BJU Int 2000; 85: 1007-1013
  14. Kosieradzki M, Ametani M, Southard JH, Mangino MJ. Is ischemic preconditioning of the kidney clinically relevant? Surgery 2003; 133: 81-90
  15. Lee CM, Carter JT, Randall HB, Hiose R, Stock PG, Melzer JS, Dafoe DC, Freise CE, Alfrey E1. The effect of age and prolonged cold ischemia times on the national allocation of cadaveric renal allografts. J Surg Res 2000; 91: 83-88 https://doi.org/10.1006/jsre.2000.5921
  16. Melanie LB, Robert GF, James ES, Dominic PG, Jeff Sc. Effects of antioxidant supplementation on blood cyclosporine A and glomerular filtration rate in renal transplant recipients. Nephrol Dial Transplant 2005; 20: 1970-1975 https://doi.org/10.1093/ndt/gfh875
  17. Salahudeen AK, Wang C, Kanji VK. Comparative study of the effect of 21-aminosteroid and vitamin E on models of acute renal injury. Free Radic Bioi Med 1996; 21: 691-697 https://doi.org/10.1016/0891-5849(96)00126-8
  18. Sekhon CS, Sekhon BK, Singh I, Orak JK, Singh AK. Attenuation of renal ischemia!reperfusion injury by a triple drug combination therapy. J Nephrol 2003;16: 63-74
  19. Shanley PF, Rosen MD, Brezis M, Silva P, Epstein FH, Rosen S. Topography of focal proximal tubular necrosis after ischemia with reflow in the rat kidney. Am J Pathol 1986; 22: 462-468
  20. Takada M, Nadeau KC, Shaw GO, Marqueette KA, Tilney NL. The cytokine-adhesion molecule cascade in ischemia! reperfusion injury of the rat kidney. Inhibition by soluble P-selectin ligand. J Clin Invest 1987; 99: 2682-2690 https://doi.org/10.1172/JCI119457
  21. Uysal F, Girgin FK, Tuzun S, Aldemir S, Sozmen EY. Effect of vitamin E on antioxidant enzymes and nitric oxide in ischemia-reperfused kidney injury. Biochem Mol Biol lnt 1998; 44: 1255-1263
  22. Vedder NB, Winn RK, Rice CL, Chi EY, Arfos K. Inhibition of leukocyte adherence by anti-CD 18 monoclonal antibody attenuates reperfusion injury in the rabbit ear. Proc Natl Acad Sci USA 1990; 87: 2643-2646 https://doi.org/10.1073/pnas.87.7.2643
  23. Yokozawa T, Fujii H, Kosuna K, Nonaka G. Effects of buckwheat in a renal ischemia-reperfusion model. Biosci Biotechnol Biochem 2001; 65: 396-400 https://doi.org/10.1271/bbb.65.396