Evaluation on Efficacies of Sodium Hypochlorite and Benzalkonium Chloride against Listeria monocytogenes and Salmonella spp.

Listeria monocytogenes와 Salmonella spp.에 대한 Sodium Hypochlorite 및 Benzalkonium Chloride의 유효성 평가

  • Kim, Hyung-Il (Center for Food Evaluation, Korea Food and Drug Administration) ;
  • Yoon, Hae-Jung (Center for Food Evaluation, Korea Food and Drug Administration) ;
  • Choi, Hyun-Cheol (Center for Food Evaluation, Korea Food and Drug Administration) ;
  • Jeon, Dae-Hoon (Center for Food Evaluation, Korea Food and Drug Administration) ;
  • Eom, Mi-Ok (Center for Food Evaluation, Korea Food and Drug Administration) ;
  • Sung, Jun-Hyun (Center for Food Evaluation, Korea Food and Drug Administration) ;
  • Park, Na-Young (Center for Food Evaluation, Korea Food and Drug Administration) ;
  • Won, Sun-Ah (Center for Food Evaluation, Korea Food and Drug Administration) ;
  • Kim, Nam-Hee (Center for Food Evaluation, Korea Food and Drug Administration) ;
  • Sung, Deok-Wha (Center for Food Evaluation, Korea Food and Drug Administration) ;
  • Kwak, Hyo-Sun (Center for Food Evaluation, Korea Food and Drug Administration) ;
  • Kwon, Ki-Sung (Center for Food Evaluation, Korea Food and Drug Administration) ;
  • Lee, Young-Ja (Center for Food Evaluation, Korea Food and Drug Administration)
  • 김형일 (식품의약품안전청 식품본부) ;
  • 윤혜정 (식품의약품안전청 식품본부) ;
  • 최현철 (식품의약품안전청 식품본부) ;
  • 전대훈 (식품의약품안전청 식품본부) ;
  • 엄미옥 (식품의약품안전청 식품본부) ;
  • 성준현 (식품의약품안전청 식품본부) ;
  • 박나영 (식품의약품안전청 식품본부) ;
  • 원선아 (식품의약품안전청 식품본부) ;
  • 김남희 (식품의약품안전청 식품본부) ;
  • 성덕화 (식품의약품안전청 식품본부) ;
  • 곽효선 (식품의약품안전청 식품본부) ;
  • 권기성 (식품의약품안전청 식품본부) ;
  • 이영자 (식품의약품안전청 식품본부)
  • Published : 2007.06.30

Abstract

Disinfectants/sanitizers, showing the bactericidal activity of $5log_{10}$ reduction against E. coli ATCC 10536 and Staphylococcus aureus ATCC 6538 used far assessing disinfectants/sanitizers efficacies, were examined whether showing similar efficacies against Salmonella spp. and Listeria monocytogenes, the major food poisoning bacteria, isolated from foods in current Korean market. The bactericidal efficacies on sodium hypochlorite and benzalkonium chloride were assessed by quantitative suspension tests in both 'clean' and 'dirty' conditions, respectively. Test organisms were consisted of E. coli ATCC 10536, Staphylococcus aureus ATCC 6538, Salmonella typhimurium ATCC 13311, Salmonella Enteritidis ATCC 13076, Listeria monocytogenes ATCC 19111, 10 kinds of Salmonella spp. from foods, and 11 kinds of Listeria monocytogenes from foods. More than $5log_{10}$ reduction in viable count for all strains was only achieved with benzalkonium chloride at the tolerance exemption concentration under dirty conditions. However, all strains were achieved more than $5log_{10}$ reduction under clean conditions.

살균소독력 평가균주로 사용되는 E. coli ATCC 10536과 Staphylococcus aureus ATCC 6538균에 대하여 5 logCFU/ml 감소의 살균소독력을 보이는 살균소독제가 국내에서 식중독을 일으키고 있는 식중독 원인균 중 Salmonella spp.와 Listeria monocytogenes에 대하여도 같은 살균소독력을 나타내는지 비교평가하였다. 청정조건 및 오염조건에서 현탁액시험법을 사용하여 차아염소산나트륨과 염화벤잘코늄의 살균소독력을 평가하였다. 시험균주로는 E. coli ATCC 10536, Staphylococcus aureus ATCC 6538, Salmonella typhimurium ATCC 13311, Salmonella Enteritidis ATCC 13076, Listeria monocytogenes ATCC 19111, Salmonella spp. 10균주 및 Listeria monocytogenes 11균주를 사용하였다. 오염조건에서는 염화벤잘코늄만이 살균소독제의 유효성분 사용범위 농도 내에서 5 log 감소 이상의 살균소독력을 보였으나, 청정조건에서는 실험대상 균주 전부 5 log감소 이상의 살균소독력을 보였다.

Keywords

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