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뽕잎 추출물을 이용한 동충하초(Paecilomyces japonica) 균사체 배양액의 멜라닌 합성 억제 효과

nhibition of Melanin Synthesis by Mycelial Culture Broth of Paecilomyces japonica in the Mulberry

  • 박상상 (진주산업대학교 미생물공학과) ;
  • 류영배 (경상대학교 응용생명과학부) ;
  • 이영훈 (진주산업대학교 미생물공학과) ;
  • 조용운 (진주산업대학교 미생물공학과) ;
  • 조수정 (진주산업대학교 미생물공학과) ;
  • 최영주 (신라대학교 식품영양학과) ;
  • 박기훈 (경상대학교 응용생명과학부) ;
  • 갈상완 (진주산업대학교 미생물공학과)
  • Park, Sang-Sang (Department of Microbiological Engineering, JinJu National University) ;
  • Ryu, Young-Bae (Department fo Applied Life Science, Gyeongsang National University) ;
  • Lee, Young-Hoon (Department of Microbiological Engineering, JinJu National University) ;
  • Cho, Yong-Un (Department of Microbiological Engineering, JinJu National University) ;
  • Cho, Soo-Jeong (Department of Microbiological Engineering, JinJu National University) ;
  • Choi, Young-Ju (Department of Food Nutrition, Silla University Busan) ;
  • Park, Ki-Hoon (Department fo Applied Life Science, Gyeongsang National University) ;
  • Gal, Sang-Wan (Department of Microbiological Engineering, JinJu National University)
  • 발행 : 2007.06.25

초록

본 연구는 뽕잎 추출물을 이용한 동충하초 균사체 배양액의 in vivo, in vitro멜라닌 생성 억제 효과를 조사한 것이다. 뽕잎 추출물을 이용한 동충하초 균사체 배양액은 10~50%의 농도 의존적으로 시험관 내에서 멜라닌 합성관련 효소활성의 저해를 나타냈다. 뽕잎 추출물 자체는 mouse melanoma cell (B16BL6)에 대한 세포독성을 보였으며 IC$_{50}$%값이 약 7% 농도로 나타난 반면, 뽕잎 추출물을 이용한 동충하초 균사체배양액은 50% 농도에서도 어떠한 세포독성도 관찰되지 않음을 확인할 수 있었다. 또한 B16BL6 mouse melanoma cell의 세포 내 멜라닌 생합성 억제에 대해서도 높은 활성을 나타내었다. 따라서, 뽕잎 추출물을 이용한 동충하초 균사체 배양액은 세포에 대한 독성이 전혀 없으며, 멜라닌 생합성 저해 작용이 우수하여 피부 미백용 화장료 조성물로서의 이용 가능성이 확인 되었다.

This study was carried out to investigate the whitening effect of mycelial culture broth of P. japonica in the mulberry leaf extract. The culture broth inhibited melanin synthesis in vitro in the concentration dependent manner of 10 to 50%. The mulberry leaf extract itself has a cytotoxicity to the mouse melanoma cell, B16BL6 with the value of IC$_{50}$ of 7% concentration. But the culture broth of P. japonica in the mulberry leaf extract did not show any cytotoxicity up to 50%. Therefore, we concluded the mycelial culture broth of P. japonica in the extract of mulberry leaf can be used as a whitening cosmetic resource.

키워드

참고문헌

  1. Asano, N., K. Oseki, E. Tomioka, H. Kizu and K. Matsui. 1994. N-containing sugar from the Morus alba abd their glycosidase inhibitory activities. Carbohydrate Research 259, 243-255 https://doi.org/10.1016/0008-6215(94)84060-1
  2. Bao, Z. D., Z. G. Wu and F. Zheng. 1994. Amelioration of aminoglyvoside nephrotoxicity by Cordyceps sinensis in old patient. Chin. J. Integr. Med. 14, 271-273
  3. Bell, A. A. and M. H. Weeler. 1986. Biosynthesis and function of fungal melanin. Amm. Rev. Phytophathol. 240, 411-451
  4. Dietschy, J. M. and J. D. Wilson. 1970. Regulation of cholesterol metabolism. N Engl J Med. 282(21), 1179-83 https://doi.org/10.1056/NEJM197005212822105
  5. Dooley. T. P., R. C. Gadwood, K. Kilgore and L. M. Thomasco. 1994. Development of an in vitro primary screen for skin depigmentation and antimelanoma agents. Skin Pharmacol. 7(4), 188-200 https://doi.org/10.1159/000211294
  6. Gordon, P. R., C. P. Mansur and B. A. Gilchrest. 1989. Regulation of human melanocyte growth, dendricity, and melanization by keratinocyte derived factors. J. Invest. Dermatol. 92, 566-572
  7. Han D. S., N. H. Soung and Y. U. Kim, 1999. The prosent condition of Dong-Chong-Ha-Cho, issue and research plan. Korean Society of Food Science and Trchnology 32(4), 67-71
  8. Hearing, V. J. and T. M. Ekel. 1976. Mammalian tyrosinase. Biochem. J. 157, 549-557 https://doi.org/10.1042/bj1570549
  9. Katai, K. 1942. Trace components in mulberry leaves. J. Chem. Soc. Jph. 18, 379-383
  10. Khaw, K. T. and C. E. Barret. 1987. Dietary fiber and reduced ischemic heart disease mortality in men and women. Am J. Epideilol. 126, 1093-1095 https://doi.org/10.1093/oxfordjournals.aje.a114748
  11. Kim, S. K., Y. C. Kim and S. Y. Kim. 1999. Antihyperlipidemic effects of mulberry leaves in adult females. Soonchunhyang J. Nat. Sci. 5, 167-171
  12. Kim, S. Y., W. C. Lee, H, B, Kim, A. J. Kim and S. K. Kim. 1998. Antihyperlipidemic effect of methanol extracts from mulberry leaves in cholesterol-induced hyperlipidemia rats. J. Korean Soc. Food Sci. Nutr. 27, 1217-1222
  13. Kimura, M., F. Chen, N. Nakashima, I. Kimura, N. Asano and S. Koya. 1995. Antihyperglycemic effect of N-containing sugar derived form mulberry leaves in STZ-induced diabetic mice. J. Trad Med. 12, 214-216
  14. Kondo, Y. 1957 Trace constituents of mulberry leaves. Nippon Sanshigaku Zasshi. 26, 349-353
  15. Lee, W. C., A. J. Kim and S. Y. Kim. 2003. The study on the functional materials and effects of mulberry leaf. Food Science and Industry 36, 2-14
  16. Lee, Y. H., U. S. Choi, K. H. Park Y. J. Choi and S. W. Gal. 2006. Anti-wrinkle effect of mycelial culture broth of Paecilomyces japonica in the mixture of cucumber and grape extracts. Korean J. Life Science, 16(3), 516-521 https://doi.org/10.5352/JLS.2006.16.3.516
  17. Mahley, R. W, K. H. Weisgraber and T. L. Inneraritym. 1974. Characterization of the plasma lipoprotein associated with atherogenic and nonantherogenic hyperlipoproteinemia. Circ. Rev. 35, 722-723 https://doi.org/10.1161/01.RES.35.5.722
  18. Nita, A. and A. R. Young. 2005. Melanogenesis: a photoprotective response to DNA damage? Mutation Research 571, 121-132 https://doi.org/10.1016/j.mrfmmm.2004.11.016
  19. Shim. J. Y., Y. S. Lee, S. S. Lim, K. H. Shin, J. E. Hyun, S. Y. Kim and E. B. Lee. 2000. Pharmacological Activities of Paecilomyces japonica, A New Type Cordyceps sp. Korean Journal of Pharmacognosy. 31(2), 163-167
  20. Tajima S., A. Ura-Ishiko and A. Hayashi. 1996. Melanogenesis, biosynthetic phenotype of fibronectin and collagen and migrating activity in cloned B16 mouse melanoma cells. J. Dermatological Science 12(1), 24-30 https://doi.org/10.1016/0923-1811(95)00455-6

피인용 문헌

  1. Antioxidant Activities of Extracts from Fermented Mulberry (Cudrania tricuspidata) Fruit. and Inhibitory Actions on Elastase and Tyrosinase vol.18, pp.2, 2011, https://doi.org/10.11002/kjfp.2011.18.2.236
  2. Ingredients Analysis and Biological Activity of Fermented Angelica gigas Nakai by Mold vol.20, pp.9, 2010, https://doi.org/10.5352/JLS.2010.20.9.1385
  3. Melanogenesis effect ofCordyceps militarisculture broth on the melanin formation of B16F0 melanoma cells 2011, https://doi.org/10.3109/08923973.2011.611810
  4. Effects of Dietary Supplementation of Mulberry Leaves and Silkworm Excreta Extracts on Weight Performance, Blood Characteristics and Cecal Microflorae of Chicks vol.50, pp.2, 2012, https://doi.org/10.7852/jses.2012.50.2.150
  5. Antioxidative Activity and Chemical Characteristics of Cordycepin-enriched Cordyceps militaris JLM0636 Powder vol.23, pp.2, 2013, https://doi.org/10.5352/JLS.2013.23.2.249
  6. Biological Activity and Chemical Characteristics of Fermented Acanthopanax senticosus by Mold vol.22, pp.12, 2012, https://doi.org/10.5352/JLS.2012.22.12.1704