한국식품영양학회지 (The Korean Journal of Food And Nutrition)

  • 제20권2호
  • /
  • Pages.240-244
  • /
  • 2007
  • /
  • 1225-4339(pISSN)
  • /
  • 2287-4992(eISSN)
한국식품영양학회 (The Korean Society of Food and Nutrition)
권호 표지

B16 마우스 흑색 종양 세포에서 빈랑(Areca catechu) 추출물이 티로시나아제 유전자에 미치는 효과

The Effects of Areca catechu Extracts on Tyrosinase Gene Expression in B16 Mouse Melanoma Cells

  • 발행 : 2007.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

In this study, we found that the methanolic extract of Areca catechu repressed expression of the tyrosinase gene in B16 mouse melanoma cells containing a tyrosinase promoter. Extract concentrations of 100 ${\mu}$g/ml and 500 ${\mu}$g/ml exhibited tyrosinase gene expression rates of aproximately 62% and 48%, respectively, compared to the control. The fraction layers consisting of ethyl acetate, butyl alcohol, and water showed repressive effects on the tyrosinase gene. In particular, the butyl alcohol fraction highly repressed at 100 ${\mu}$g/ml and 500 ${\mu}$g/ml. In the MTT assay, the methanolic extracts exhibited very low cytotoxicities at 1 ${\mu}$g/ml, 10 ${\mu}$g/ml, and 100 ${\mu}$g/ml.

키워드

참고문헌

  1. Lee, KS, Kim, SH, Kim, SS, Park, SS, Chun, JY and Shin YS. Antimicrobial activity of herbs related with treatments of intestinal diseases against intestinal pathogens. Kor. J. Food Nutr. 11:31-35. 1998
  2. Lee, KS, Kim, SH, Chun, SH, Park, SS, Park, JS and Shin, YS. Antimicrobial activity of Areca catachu L. extract of against intestinal pathogens. Kor. J. Food Nutr. 11:36-40. 1998
  3. 학교법인고려중앙학원. 암세포에 대한 다중약제내성조절용 빈랑 추출물 및 그 제조방법. 특허공보(등록번호: 2002-0009123). 2002
  4. 안동대학교 산학협력단. 빈랑자 추출물을 함유하는 트롬빈 저해 혈전증 예방 및 치료용 조성물. 특허공보(등록번호: 10-0674602). 2007
  5. Park, JH, Shin, YG, Shin, UK, Baek, SK, Lee, SK, Chung, MH and Park, Yl. Tyrosinase inhibition activity of some herbal drugs. Yakhak Hoeji 41:518-523. 1997
  6. Bae, JS. Dyeing properties of cotton and wool fabrics with betel palm tree. Kor. Home Economics Association. 42:63-72. 2004
  7. Aroca, P, Urabe, K, Kobayashi, K, Taskamoto, K and Hearing, VJ. Melanin biosynthesis patterns of following hormonal stimulation. J. Biol. Chem. 268:25650-25655. 1993
  8. Paval, S. Dynamics of melanogenesis intermediates. J. Invest. Dermatol. 100:162S-165S. 1993 https://doi.org/10.1111/1523-1747.ep12465095
  9. Jimenez-Cervantes, C, Solano, F, Kobayashi, T, Urabe, K, Hearing, VJ, Lozano, J and Garcia-Barron, C. A new enzymatic function in the melanogenic pathway. J. Biol. Chem. 269:17993-18001. 1994
  10. Choi, BW, Lee, BH, Kang, KJ, Lee, ES and Lee, NH. Screening of the tyrosinase inhibitors from marine algae and medicinal plants. Kor. J. Pharm. 29:237-242. 1998
  11. Baurin, N, Amoult, E, Scior, T, Do, QT and Bernard, P. Preliminary screening of some tropical plants for anti-tyrosinase activity. J. Ethnopharmacol. 82:155-158. 2002 https://doi.org/10.1016/S0378-8741(02)00174-5
  12. Tomohiro, Y, Hiroyuki, N, Yasuo, K and Masako, M. The inhibitory effect of glabridin from licorice extracts on melanogenesis and inflammation. Pigment Cell Res. 11:355-361. 1998 https://doi.org/10.1111/j.1600-0749.1998.tb00494.x
  13. Isao, Kubo and Ikuyo, Kinst-Hori. 2-Hydroxy-4-methoxybenzaldehyde a potent tyrosinase inhibitor from Afiican medicinal plants. Planta Medica. 65:19-22. 1999 https://doi.org/10.1055/s-1999-13955
  14. Lee, SH, Choi, SY, Kim, HC, Hwang, JS, Lee, BG, Gao, JJ and Kim, SY. Mulberroside F isolated from the leaves of Morus alba inhibits melanin biosynthesis. Biol. Pharm. Bull. 25:1045-1048. 2002 https://doi.org/10.1248/bpb.25.1045
  15. Lee, KT, Lee, KS, Jeong, JH, Jo, BK, Heo, MY and Kim, HP. Inhibitory effects of Ramulus mori extracts on melanogenesis. J. Cosmet. Sci. 54: 133-142. 2003
  16. Chin, JE, Sun, HS, Lee, KJ, Choi, TJ, Ko, YS, Sohn, HJ, Kim, JJ, Jeon, BH and Lee, BH. Inhibitory effects of some medicinal plant extracts on the tyrosinase promoter activity on B16 mouse melanoma cells. International J. Oriental Medicine. 1:6-13. 2000
  17. Chin, JE and Cho, NC. Effect of Houttuynia cordata extracts on tyrosinase gene expression. J. Kor. Soc. Food Sci. Nutr. 34:1284-1288. 2005 https://doi.org/10.3746/jkfn.2005.34.8.1284
  18. Chin, JE and Kim, KC. Effect of chestnut bark extracts on tyrosinase gene expression. Kor. J. Sanitation 20:10-16. 2005
  19. Cho, NC, Yoon, YH, Lee, HJ, Shon, HJ, Kim, YK, Choi, KH, Ra, MS, Cho, YK, Lee, BH and Chin, JE. Effect of onion(Allium cepa L.) extract on tyrosinase gene expression. Kor. J. Food & Nutr. 14:228-232. 2001
  20. Lee, HB, Bai, S and Chin, JE. Inhibitory effect of Lithospermum erythrorhizon extracts on melanin biosynthesis. J. Kor. Soc. Food Sci. Nutr. 34:1325-1329. 2005 https://doi.org/10.3746/jkfn.2005.34.9.1325
  21. Mosmann, TJ. Rapid colormetric assay for cellular growth and survival application to proiferation and cytotoxicity assay. Immunol. Methods 63:55-63. 1983