Journal of Periodontal and Implant Science

  • 제37권1호
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  • Pages.53-64
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  • 2007
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  • 2093-2278(pISSN)
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  • 2093-2286(eISSN)
대한치주과학회 (Korean Academy of Periodontology)
권호 표지

미니돼지에서 Acellular dermal matrix에 배양된 치주인대섬유모세포을 이용한 자가치아이식술: 치주인대로써의 잠재력에 대한 조직학적 평가

Autotransplantation using the acellular dermal matrix seeded by periodontal ligament fibroblasts in minipig: histological evaluation as potential periodontal ligament substitutes

  • 유상준 (조선대학교 치과대학 치주과학 교실) ;
  • 김병욱 (조선대학교 치과대학 치주과학 교실, 구강생물학 연구소) ;
  • 박주철 (조선대학교 치과대학 구강조직학 교실, 구강생물학연구소) ;
  • 장현선 (조선대학교 치과대학 치주과학 교실, 구강생물학 연구소)
  • Yu, Sang-Joun (Department of Periodontology, College of Dentistry, Chosun University) ;
  • Kim, Byung-Ock (Department of Periodontology, College of Dentistry, Chosun University, Oral Biology Research Institute) ;
  • Park, Joo-Cheol (Department of Oral Histology, College of Dentistry, Chosun University, Oral Biology Research Institute) ;
  • Jang, Hyun-Seon (Department of Periodontology, College of Dentistry, Chosun University, Oral Biology Research Institute)
  • 발행 : 2007.03.30
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초록

The aim of this study was to examine the possibility of periodontal ligament regeneration when autotransplantation was used by the periodontal ligament fibroblasts cultured on the acellular dermal matrix in teeth without a periodontal ligament. One minipig was used in this study. The mandibular and maxillary permanent incisors were ex-tracted for the culture of the periodontal ligament cells. The roots of the unextracted teeth were classified into a positive control group, in which the normal periodontal ligament was preserved. The roots of the extracted teeth were divided into the following two groups: The negative control group, in which the periodontal ligament had been removed and the acellular dermal matrix was not applied; and an experimental group, in which the periodontal ligament had been removed and periodontal ligament fibroblast cultured on an acellular dermal matrix was applied. The prepared teeth were transplanted, and completely submerged using physical barrier membranes. The animal was sacrificed 4 weeks after the autotransplant. The transplanted teeth were examined histologically. In this study, the periodontal ligament was normal in the positive control group, and ankylosis was discovered on the denuded root surface in the negative control group. Periodontal ligament-like connective tissue was found adjacent to the denuded root and the new cementum-like layer of hard tissue was formed in the experimental group. These results suggest that the periodontal ligament fibroblasts cultured on the acellular dermal matrix may play a role in regenerating the periodontal ligament-like tissue with new cememtum-like tissue formation.

키워드

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