식물 혼합(고들빼기, 돌미나리, 메밀, 톳, 생강) 추출물이 마우스 면역 세포 활성에 미치는 영향

Effects of a Plant Water Extract Mixture(Ixeris sonchifolia Hance, Oenanthe javanica, Fagopyrum esculentum Moench, Hizikia fusiforme, Zingiber officinale Roscoe) on Mouse Immune Cell Activation

  • 김정희 (숙명여자대학교 생활과학대학 식품영양학과) ;
  • 김현숙 (숙명여자대학교 생활과학대학 식품영양학과) ;
  • 류혜숙 (상지대학교 이공대학 식품영양학과)
  • Kim, Jung-Hee (Major in Food & Nutrition, Sookmyung Women's University) ;
  • Kim, Hyun-Sook (Major in Food & Nutrition, Sookmyung Women's University) ;
  • Ryu, Hye-Sook (Department of Food and Nutrition, Sangji University)
  • 발행 : 2007.03.31

초록

In vitro를 통한 혼합시료의 물 추출물과 에탄올 추출물을 시료로 사용하였고, 마우스 비장세포 증식능 및 활성 복강 대식세포에서 분비하는 사이토카인(IL-1 ${\beta}$, IL-6, TNF-${\alpha}$)의 분비능을 측정하였다. 그 결과 혼합시료의 물 추출물과 에탄올 추출물 모두 5${\sim}$250 ${\mu}$g/ml의 농도로 첨가했을 때 세포증식을 촉진하는 효과가 있는 것으로 나타났고, 물 추출물인 경우 고농도인 1,000 ${\mu}$g/ml에서, 에탄올 추출물인 경우는 500${\sim}$1,000 ${\mu}$g/ml 이상의 고농도 첨가시 세포증식이 억제되는 효과를 보였다. 복강 대식세포의 사이토카인 분비량을 측정한 결과, 혼합 물 추출물과 에탄올 추출물 모두에서 대조군보다 높은 분비량을 보였다. IL-1${\beta}$ 생성량 검색 결과, 물 추출물 투여군 10 ${\mu}$g/ml 농도 첨가에서 유의적(p<0.05)으로 높은 분비능을 보여주었고, 에탄올 추출물을 10 ${\mu}$g/ml와 100 ${\mu}$g/ml 첨가시에도 대조군보다 높은 분비량을 보였다. IL-6의 결과에서도, 물 추출물 10 ${\mu}$g/ml을 첨가했을 때 유의적(p<0.05)으로 높은 분비량을 보였고, TNF-${\alpha}$의 경우, 물 추출물의 10 ${\mu}$g/ml와 100 ${\mu}$g/ml의 농도에서 대조군에 비해 높은 TNF-${\alpha}$ 분비능을 보였다. 이상의 결과에 따르면, 혼합 물 추출물이 비장세포 증식능과 복강 대식세포에 의한 사이토카인 분비능을 상승시킴으로서 면역 기관의 주요 기능을 증진시키는 것으로 사료된다.

Ixeris sonchifolia Hance(Godulbaegi), Oenanthe javanica(Dolminari), Fagopyrum esculentum Moench(Buckwheat), Hizikia fusiforme(Seaweed Fusiforme) and Zingiber officinale Roscoe(Ginger) have all been used as one of the traditional remedies as well as food source. There are few studies However, on their immunomodulating effects have been reported. We previously reported that ex vivo supplementation of each of the Ish, Oj, Fem, Hf and Zor water extracts enhanced the splenocytes proliferation compared to the control group. In this study, the combined immunomodulative effects of a plant water extract mixture containing these five food sources(Ish+Oj+Fem+Hf+Zor) was compared to the individual effect of each. The production of cytokine(IL-1${\beta}$, IL-6, and TNF-${\alpha}$), secreted by macrophages stimulated with LPS or without, were detected via ELISA assay using a cytokine kit. After 48hrs of incubation with mitogen(ConA or LPS) stimulation, the mouse splenocyte proliferation in the experimental group had significantly increased at two different concentrations compared to the control group. The results of this study may suggest that the supplementing with a plant water extract mixture could regulate immune function by increasing splenocyte proliferation as well as enhance immune function by regulating the cytokine production capacity activated macrophages in mice.

키워드

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