Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Antitumor Activity and Antioxidant Role of Ichnocarpus frutescens Against Ehrlich Ascites Carcinoma in Swiss Albino Mice

  • Dash, Deepak K (College of Pharmaceutical Sciences) ;
  • Nayak, Siva S (College of Pharmaceutical Sciences) ;
  • Samanta, Soma (Division of Pharmaceutical Chemistry, Department of Pharmaceutical Technology, Jadavpur University) ;
  • Ghosh, Tirtha (Institute of Pharmacy and Technology) ;
  • Jha, Tarun (Division of Pharmaceutical Chemistry, Department of Pharmaceutical Technology, Jadavpur University) ;
  • Maiti, Bhim C (Division of Pharmaceutical Chemistry, Department of Pharmaceutical Technology, Jadavpur University) ;
  • Maity, Tapan K (Division of Pharmaceutical Chemistry, Department of Pharmaceutical Technology, Jadavpur University)
  • Published : 2007.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

The plant Ichnocarpus frutescens (Linn) R.Br. (Family-Apocynaceae) has been indicated for the treatment of various diseases, one amongst it is cancer. The purpose of this study was to investigate experimentally the possible antitumor activity and antioxidant role of Ichnocarpus frutescens in the mice transplanted with Ehrlich ascites carcinoma (EAC). The chloroform and methanol extract of whole plant of Ichnocarpus frutescens (CEIF and MEIF) were administered intraperitoneally at the dose of 150 mg/kg and 300 mg/kg, body weight per day for 7 days after 24 h of tumor inoculation in mice. Treatment with CEIF at the dose of 150 mg/kg and 300 mg/kg remarkably decreased the tumor volume, packed cell volume, viable cell count and increased the nonviable cell count of EAC tumor bearing mice when compared to e effect of MEIF at 150 mg/kg and 300 mg/kg. Further the EAC mice treated with CEIF and MEIF showed significant decrease in the level of lipid peroxidation and significant increase in the level of antioxidant enzymes such as glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT), however the decreasing and increasing capacity of CEIF was less in both doses as compared to MEIF. Based on these results, it can be concluded that the chloroform and methanol extact of Ichnocarpus frutescens exhibit significant antitumor and antioxidant activity in EAC bearing mice.

Keywords

References

  1. Abdullaev, F.I., Rivera, L.R., Roitenburd, B.V., and Espinosa, A.J., Pattern of childhood cancer mortality in Mexico. Arch. Med Res., 31, 526-531, (2000) https://doi.org/10.1016/S0188-4409(00)00094-1
  2. Aebi, H., Catalase, In Bcrgmeyer, H.U (ed.), Methods of Enzymatic Analysis, Vol. II, Academic Press, New York, 1974, pp. 673-684
  3. Asolkar, L.V., Kakkar, K.K., and Chakre, O.J., Second suppliment to Glossary of Indian Medicinal Plants with active principles (1965-81), National institute of science communication, Dr. K. S. Krishnan Marg, New Delhi, India, 1992, pp. 366
  4. Beutler, E., and Kelly, B.M., The effect of sodium nitrate on red cell glutathione. Experientia. 18, 96-97 (1963)
  5. Blanco, E.J.C., Cuendet, M., Park, E.J., Su, B.N., Riverocruz, J.F., Farnsworth, N.R., Pezzuto, J.M., and Kinghorn, A.D., Potential cancer chemopreventive agents from Arbutus unedo. Nat. Prod. Res. 20, 327-334 (2006) https://doi.org/10.1080/14786410500161205
  6. Campo, G.M., Squadrito, F.S., Ceccarelli, N., Calo, A., Avenoso, S., Campo, G., Squadrito, D., Aitavilla. Reduction of carbon tertrachloride-induced rat liver injury by IRFI 042, a novel duel vitamin E-like antioxidant. Free. Radical. Res. 34, 379-393 (2001) https://doi.org/10.1080/10715760100300321
  7. Camporti, M., Biology of Disease. Lipid peroxidation and cellular damage in toxic liver injury. Lab. Invest. 53, 599-623 (1985)
  8. Chatterjee, A. and Pakrashi, SC., The Treatise on Indian Medicinal Plants, Vol. IV, Publications and information Directorate, New Delhi, India, 1995, pp. 110-112
  9. Cragg, G.M., Newman, D.J., and Snader, K.M., Natural products in drug discovery and development. J. Nat. Prod. 60, 52-60 (1997) https://doi.org/10.1021/np9604893
  10. DeFeudis, F.V., Papadopoulos, V., and Drieu, K., Ginkgo biloba extracts and cancer: a research area in its infancy. Fundam. Clin. Pharmacol. 17, 405-17 (2003) https://doi.org/10.1046/j.1472-8206.2003.00156.x
  11. DeWys, W.D., Pathophysiology of cancer cachexia: current understanding and areas for future research. Cancer. Res. 42, 721s-726s (1982)
  12. Farnsworth, N.R., Akerele, O., Bingel, A.S., Soejarto, D.D., and Guo, Z., Medicinal plants in therapy. World Health Organization Bulletin, 63, 965-981 (1985)
  13. Gerzon, K., Anticancer Agents Based on Natural Product Models, In Cassady, J.M., and Douros, J.D. (eds.), Academic Press, New York, 1980, pp. 271-317
  14. Gayen, S., Debnath, B., Samanta, S., Ghosh, B., Basu, A., Jha, T., 1, 5, N, N'- Disubstituted -2- (Substituted Benzenesulphonyl)- Glutamamide analogues as anticancer agents - 3: Synthesis, biological screening and QSAR study. Int. Electro. J. Mol. Des. 4, 556-578 (2005)
  15. Ghosh, M.N., Fundamentals of Experimental Pharmacology, Second edition, Scientific Book Agency, Calcutta, India, 1984, pp. 177-211
  16. Jardine, I., Anticancer Agents Based on Natural Product Models, In Cassady J.M., and Douros, J.D. (eds.), Academic Press, New York, 1980, pp. 319-351
  17. Kakkar, P., Dos, B., Viswanathan, P.N., Maehly. A.C., and Chance, B., In Glick, D. (ed.), Methods of biomedical analysis, Vol. I, Interscience, New York, 1954, p.357
  18. Khan, M.S.Y., Javed, K., and Khan, M.H., Chemical constituents of the leaves of Ichnocarpus frutescens R. Br. J. Ind. Chem. Soc. 72, 65-66 (1995)
  19. Kim, J., and Park, E.J., Cytotoxic anticancer candidates from natural resources. Curr. Med. Chem. Antican. Agent, 2, 485-537 (2002) https://doi.org/10.2174/1568011023353949
  20. Kinghorn, A.D. and Balandrin, M.F., Human Medical Agents from Plants, American Chemical Society Symposium Series 534, American Chemical Society, Washington, DC, 1993, pp. 80-95
  21. Kirtikar, K.R. and Basu, B.D., Indian Medicinal Plants, Vol.II, Lalit Mohan Basu Publications, Allahabad, India, 1998, pp. 1590-1592
  22. Lakshmi, D.K.M., Rao, E.V., and Rao, D.V., Triterpenoid constituents of Ichnocarpus frutescens. Indian Drugs 22, 552-553, (1985)
  23. Mascarenbas, M., Structure-activity characterization, a quick method to screen mushrooms for the presence of antitumor glucans. Mushroom Res. 3, 77-80 (1994)
  24. Nadkarni, K.M., The Indian Materia Medica, Vol. I, Bombay Popular Prakashan, Bombay, India, 1976, p. 674
  25. Ohkawa, H., Onishi, N., and Yagi, K., Assay for lipid peroxidation in animal tissue by thiobarbituric acid reaction. Anal. Biochem. 95, 351-58 (1979) https://doi.org/10.1016/0003-2697(79)90738-3
  26. Plaa, G.L. and Wistshi, H., Chemicals, drugs and lipid peroxidation. Annu. Rev. Pharmacol. Taxicol. 16, 125-41 (1976) https://doi.org/10.1146/annurev.pa.16.040176.001013
  27. Prasad, S.B. and Giri, A., Antitumor effect of cisplatin against murine ascites Dalton's lymphoma. Ind. J. Expt. Biol. 32, 155-162 (1994)
  28. Rajeshwar, Y., Gupta, M., and Mazumder, U.K., Antitumor activity and in vivo antioxidant status of Mucuna pruriens (Fabaceae) seeds against Ehrlich ascites carcinoma in swiss albino mice. I.J.P.T., 4, 46-53 (2005)
  29. Richardson, M.A., Sanders T., Palmer, J.L., Greisinger, A., and Singletary, S.E., Complementary Alternative medicine use in a comrehensive cancer center and the implications for oncology. J. Clin. Oneal., 18, 2505-2514 (2000) https://doi.org/10.1200/JCO.2000.18.13.2505
  30. Rushmore, T.H. and Picket, C.B., Glutathione-S-transferase, structure, regulation, and therapeutic implication. J. Biol. Chem. 268, 11475-11478 (1993)
  31. Sinclair, A.J., Barnett, A.H., and Lunie, J., Free radicals and antioxidant systems in health and disease. Brit. J. Hospl. Med. 43, 334-344 (1990)
  32. Singh, R.P. and Singh R.P., Flavonoids of the flowers of Ichnocarpus frutescens. J. Indian. Chem. Soc. 64, 715-716 (1987)
  33. Suffness, M. and Pezzuto, J.M., In: Methods in Plant Biochemistry, Vol. VI, Academic Press, New York, p. 71, 1991
  34. Sun, Y., Oberley, L.W., Elwell, J.H., and Sierra, R.E., Antioxidant enzyme activities in normal and transformed mice liver cell. Int. J. Cancer. 44, 1028-1033 (1989) https://doi.org/10.1002/ijc.2910440615
  35. Takeoka, G.R. and Dao, L.T., Antioxidant constituent of almond [Prunus dulcis (Mill.) D.A. Webb.] hulls. J. Agric. Food. Chem. 51, 496-501 (2003) https://doi.org/10.1021/jf020660i
  36. Wall, M.E. and Wani M.C., Human Medicinal Agents from Plants, In Kinghorn, A.D., and Balandrin, M.F. (eds.), American Chemical Society Symposium Series 534, 1993, pp. 149-169
  37. Yagi, K., Lipid peroxide and human disease. Chem. Physiol. Lip. 45, 37-51 (1987)